First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms
Bromus catharticus Vahl. has been used as a valuable forage crop, but it has also been noted as a weed of winter crops and an invader in several countries. In Argentina, a putative glyphosate-resistant population of B. catharticus was identified as a consequence of the lack of effective control with...
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| Format: | Article |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.617945/full |
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| author | Marcos Yanniccari Marcos Yanniccari José G. Vázquez-García María E. Gómez-Lobato María E. Gómez-Lobato Antonia M. Rojano-Delgado Pedro L. da C. A. Alves Rafael De Prado |
| author_facet | Marcos Yanniccari Marcos Yanniccari José G. Vázquez-García María E. Gómez-Lobato María E. Gómez-Lobato Antonia M. Rojano-Delgado Pedro L. da C. A. Alves Rafael De Prado |
| author_sort | Marcos Yanniccari |
| collection | DOAJ |
| description | Bromus catharticus Vahl. has been used as a valuable forage crop, but it has also been noted as a weed of winter crops and an invader in several countries. In Argentina, a putative glyphosate-resistant population of B. catharticus was identified as a consequence of the lack of effective control with glyphosate in the pre-sowing of wheat. Plant survival and shikimate accumulation analysis demonstrated a lower glyphosate-sensitivity of this population in comparison to a susceptible B. catharticus population. The resistant population was 4-fold more resistant to glyphosate than its susceptible counterpart. There was no evidence of target-site mechanisms of glyphosate resistance or an enhanced capacity to metabolize glyphosate in the resistant population. However, the resistant plants showed a lower foliar retention of glyphosate (138.34 μl solution g−1 dry weight vs. 390.79 μl solution g−1 dry weight), a reduced absorption of 14C-glyphosate (54.18 vs. 73.56%) and lower translocation of 14C-glyphosate from the labeled leaf (27.70 vs. 62.36%). As a result, susceptible plants accumulated a 4.1-fold higher concentration of 14C-glyphosate in the roots compared to resistant plants. The current work describes the first worldwide case of glyphosate resistance in B. catharticus. A reduced foliar retention of herbicide, a differential rate of glyphosate entry into leaves and an altered glyphosate translocation pattern would be the most likely mechanisms of glyphosate exclusion. |
| first_indexed | 2024-12-16T07:20:35Z |
| format | Article |
| id | doaj.art-4ce532378dc04b98b659498f625f978b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T07:20:35Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-4ce532378dc04b98b659498f625f978b2022-12-21T22:39:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-02-011210.3389/fpls.2021.617945617945First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance MechanismsMarcos Yanniccari0Marcos Yanniccari1José G. Vázquez-García2María E. Gómez-Lobato3María E. Gómez-Lobato4Antonia M. Rojano-Delgado5Pedro L. da C. A. Alves6Rafael De Prado7National Scientific and Technical Research Council, Buenos Aires, ArgentinaChacra Experimental Integrada Barrow, MDA-INTA, Tres Arroyos, ArgentinaDepartment of Agricultural Chemistry and Edaphology, University of Cordoba, Cordoba, SpainNational Scientific and Technical Research Council, Buenos Aires, ArgentinaPlant Physiology Institute (INFIVE), National University of La Plata, La Plata, ArgentinaDepartment of Agricultural Chemistry and Edaphology, University of Cordoba, Cordoba, SpainSchool of Agricultural and Veterinarian Sciences, Sâo Paulo State University (Unesp), Jaboticabal, BrazilDepartment of Agricultural Chemistry and Edaphology, University of Cordoba, Cordoba, SpainBromus catharticus Vahl. has been used as a valuable forage crop, but it has also been noted as a weed of winter crops and an invader in several countries. In Argentina, a putative glyphosate-resistant population of B. catharticus was identified as a consequence of the lack of effective control with glyphosate in the pre-sowing of wheat. Plant survival and shikimate accumulation analysis demonstrated a lower glyphosate-sensitivity of this population in comparison to a susceptible B. catharticus population. The resistant population was 4-fold more resistant to glyphosate than its susceptible counterpart. There was no evidence of target-site mechanisms of glyphosate resistance or an enhanced capacity to metabolize glyphosate in the resistant population. However, the resistant plants showed a lower foliar retention of glyphosate (138.34 μl solution g−1 dry weight vs. 390.79 μl solution g−1 dry weight), a reduced absorption of 14C-glyphosate (54.18 vs. 73.56%) and lower translocation of 14C-glyphosate from the labeled leaf (27.70 vs. 62.36%). As a result, susceptible plants accumulated a 4.1-fold higher concentration of 14C-glyphosate in the roots compared to resistant plants. The current work describes the first worldwide case of glyphosate resistance in B. catharticus. A reduced foliar retention of herbicide, a differential rate of glyphosate entry into leaves and an altered glyphosate translocation pattern would be the most likely mechanisms of glyphosate exclusion.https://www.frontiersin.org/articles/10.3389/fpls.2021.617945/fullBromeEPSPS geneshikimateglyphosate absorptionglyphosate translocation |
| spellingShingle | Marcos Yanniccari Marcos Yanniccari José G. Vázquez-García María E. Gómez-Lobato María E. Gómez-Lobato Antonia M. Rojano-Delgado Pedro L. da C. A. Alves Rafael De Prado First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms Frontiers in Plant Science Brome EPSPS gene shikimate glyphosate absorption glyphosate translocation |
| title | First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms |
| title_full | First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms |
| title_fullStr | First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms |
| title_full_unstemmed | First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms |
| title_short | First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms |
| title_sort | first case of glyphosate resistance in bromus catharticus vahl examination of endowing resistance mechanisms |
| topic | Brome EPSPS gene shikimate glyphosate absorption glyphosate translocation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.617945/full |
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