GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma
One of the most prevalent malignant primary brain tumors is primary glioma. Although glutathione peroxidase 8 (GPX8) is intimately associated with carcinogenesis, its function in primary gliomas has not yet been thoroughly understood. Here, we leveraged Chinese Glioma Genome Atlas (CGGA), The Cancer...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/8025055 |
| _version_ | 1798032823857184768 |
|---|---|
| author | Zhao-shou Yang Qin Yang Xiao-xiao Sun Kui Xiong Xiao-ting Zhu Yi-chen Wang Qian-yao Ren Guo-hui Wu Shi-min Wang Xu-qin Cao Xiao-rong Yang Wen-gong Jiang |
| author_facet | Zhao-shou Yang Qin Yang Xiao-xiao Sun Kui Xiong Xiao-ting Zhu Yi-chen Wang Qian-yao Ren Guo-hui Wu Shi-min Wang Xu-qin Cao Xiao-rong Yang Wen-gong Jiang |
| author_sort | Zhao-shou Yang |
| collection | DOAJ |
| description | One of the most prevalent malignant primary brain tumors is primary glioma. Although glutathione peroxidase 8 (GPX8) is intimately associated with carcinogenesis, its function in primary gliomas has not yet been thoroughly understood. Here, we leveraged Chinese Glioma Genome Atlas (CGGA), The Cancer Genome Atlas (TCGA), and Genotype-Tissue Expression (GTEx) database to investigate the association between GPX8 and overall survival (OS) of patients with primary gliomas, and our results showed that GPX8 expression was negatively correlated with OS. Moreover, the expression of GPX8 is significantly lower in normal tissue when compared to glioma tissue. According to results of univariate and multivariate analysis from CGGA using R studio, GPX8 is a valuable primary glioma prognostic indicator. Interestingly, high GPX8 expression is correlated positively with the hedgehog and kras signaling pathways and negatively with G2 checkpoint, apoptosis, reactive oxygen species (ROS) pathway, and interferon gamma pathway, which could be beneficial for the proliferation of glioma cells. Furthermore, GPX8 knockdown caused G1 cell cycle arrest, increased cell death, and reduced colony formation in U87MG and U118MG cells. In conclusion, GPX8 is a promising therapeutic target and meaningful prognostic biomarker of primary glioma. |
| first_indexed | 2024-04-11T20:20:01Z |
| format | Article |
| id | doaj.art-4ce6775b50184a1d882d97eac2a67576 |
| institution | Directory Open Access Journal |
| issn | 2314-7156 |
| language | English |
| last_indexed | 2024-04-11T20:20:01Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj.art-4ce6775b50184a1d882d97eac2a675762022-12-22T04:04:50ZengHindawi LimitedJournal of Immunology Research2314-71562022-01-01202210.1155/2022/8025055GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary GliomaZhao-shou Yang0Qin Yang1Xiao-xiao Sun2Kui Xiong3Xiao-ting Zhu4Yi-chen Wang5Qian-yao Ren6Guo-hui Wu7Shi-min Wang8Xu-qin Cao9Xiao-rong Yang10Wen-gong Jiang11The First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityZhongshan School of MedicineThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityZhongshan School of MedicineDepartment of Ultrasound and ElectrocardiogramThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityThe First Affiliated Hospital/School of Clinical Medicine of Guangdong Pharmaceutical UniversityOne of the most prevalent malignant primary brain tumors is primary glioma. Although glutathione peroxidase 8 (GPX8) is intimately associated with carcinogenesis, its function in primary gliomas has not yet been thoroughly understood. Here, we leveraged Chinese Glioma Genome Atlas (CGGA), The Cancer Genome Atlas (TCGA), and Genotype-Tissue Expression (GTEx) database to investigate the association between GPX8 and overall survival (OS) of patients with primary gliomas, and our results showed that GPX8 expression was negatively correlated with OS. Moreover, the expression of GPX8 is significantly lower in normal tissue when compared to glioma tissue. According to results of univariate and multivariate analysis from CGGA using R studio, GPX8 is a valuable primary glioma prognostic indicator. Interestingly, high GPX8 expression is correlated positively with the hedgehog and kras signaling pathways and negatively with G2 checkpoint, apoptosis, reactive oxygen species (ROS) pathway, and interferon gamma pathway, which could be beneficial for the proliferation of glioma cells. Furthermore, GPX8 knockdown caused G1 cell cycle arrest, increased cell death, and reduced colony formation in U87MG and U118MG cells. In conclusion, GPX8 is a promising therapeutic target and meaningful prognostic biomarker of primary glioma.http://dx.doi.org/10.1155/2022/8025055 |
| spellingShingle | Zhao-shou Yang Qin Yang Xiao-xiao Sun Kui Xiong Xiao-ting Zhu Yi-chen Wang Qian-yao Ren Guo-hui Wu Shi-min Wang Xu-qin Cao Xiao-rong Yang Wen-gong Jiang GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma Journal of Immunology Research |
| title | GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma |
| title_full | GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma |
| title_fullStr | GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma |
| title_full_unstemmed | GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma |
| title_short | GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma |
| title_sort | gpx8 as a novel prognostic factor and potential therapeutic target in primary glioma |
| url | http://dx.doi.org/10.1155/2022/8025055 |
| work_keys_str_mv | AT zhaoshouyang gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT qinyang gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT xiaoxiaosun gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT kuixiong gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT xiaotingzhu gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT yichenwang gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT qianyaoren gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT guohuiwu gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT shiminwang gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT xuqincao gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT xiaorongyang gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma AT wengongjiang gpx8asanovelprognosticfactorandpotentialtherapeutictargetinprimaryglioma |