Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer
To examine the proteomic background of lymph node metastasis (LNM) in gastric cancer, we performed protein expression profiling of paired non-tumor, primary tumor, and LNM tissues. Using a label-free proteomic approach, we generated protein expression profiles of 3894 unique proteins and identified...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | EuPA Open Proteomics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212968514000208 |
| _version_ | 1818619272215134208 |
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| author | Hiroshi Ichikawa Tatsuo Kanda Shin-ichi Kosugi Yasuyuki Kawachi Toshifumi Wakai Tadashi Kondo |
| author_facet | Hiroshi Ichikawa Tatsuo Kanda Shin-ichi Kosugi Yasuyuki Kawachi Toshifumi Wakai Tadashi Kondo |
| author_sort | Hiroshi Ichikawa |
| collection | DOAJ |
| description | To examine the proteomic background of lymph node metastasis (LNM) in gastric cancer, we performed protein expression profiling of paired non-tumor, primary tumor, and LNM tissues. Using a label-free proteomic approach, we generated protein expression profiles of 3894 unique proteins and identified 109 differentially expressed proteins. Functional pathway analysis of the differentially expressed proteins showed that members of the beta-3 integrin (ITGB3) pathway were significantly enriched. Aberrations of ITGB3 were reported in various malignancies; however, ITGB3 in LNM tissues has not been examined to date. Different level of ITGB3 expression was confirmed in 20 gastric cancer cases by Western blotting. We analyzed the mRNA levels of the differentially expressed proteins by using a public mRNA expression database; 38.8% of the proteins examined, including those involved in oxidation and reduction, showed correlation between protein and mRNA levels. Proteins without such correlation included factors related to cell adhesion. Our study suggests a novel role for the integrin pathway in the development of LNM in gastric cancer and indicated possible benefits of observational transcriptomic analysis for proteomic studies. |
| first_indexed | 2024-12-16T17:34:51Z |
| format | Article |
| id | doaj.art-4ce6b8cd6ba640d289bfa78ebe9948fa |
| institution | Directory Open Access Journal |
| issn | 2212-9685 |
| language | English |
| last_indexed | 2024-12-16T17:34:51Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EuPA Open Proteomics |
| spelling | doaj.art-4ce6b8cd6ba640d289bfa78ebe9948fa2022-12-21T22:22:49ZengElsevierEuPA Open Proteomics2212-96852014-06-013C18319410.1016/j.euprot.2014.03.001Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancerHiroshi Ichikawa0Tatsuo Kanda1Shin-ichi Kosugi2Yasuyuki Kawachi3Toshifumi Wakai4Tadashi Kondo5Division of Pharmacoproteomics, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanTsubame Rosai Hospital, 633, Sado, Tsubame-shi, Niigata 959-1228, JapanDivision of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1-757, Asahimachidori, Chuo-ku, Niigata-shi, Niigata 951-8510, JapanNagaoka Chuo General Hospital, 2041, Kawasakimachi, Nagaoka-shi, Niigata 940-8653, JapanDivision of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1-757, Asahimachidori, Chuo-ku, Niigata-shi, Niigata 951-8510, JapanDivision of Pharmacoproteomics, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, JapanTo examine the proteomic background of lymph node metastasis (LNM) in gastric cancer, we performed protein expression profiling of paired non-tumor, primary tumor, and LNM tissues. Using a label-free proteomic approach, we generated protein expression profiles of 3894 unique proteins and identified 109 differentially expressed proteins. Functional pathway analysis of the differentially expressed proteins showed that members of the beta-3 integrin (ITGB3) pathway were significantly enriched. Aberrations of ITGB3 were reported in various malignancies; however, ITGB3 in LNM tissues has not been examined to date. Different level of ITGB3 expression was confirmed in 20 gastric cancer cases by Western blotting. We analyzed the mRNA levels of the differentially expressed proteins by using a public mRNA expression database; 38.8% of the proteins examined, including those involved in oxidation and reduction, showed correlation between protein and mRNA levels. Proteins without such correlation included factors related to cell adhesion. Our study suggests a novel role for the integrin pathway in the development of LNM in gastric cancer and indicated possible benefits of observational transcriptomic analysis for proteomic studies.http://www.sciencedirect.com/science/article/pii/S2212968514000208Gastric cancerLymph node metastasisLabel-free proteomicsBeta-3 integrinTranscriptome |
| spellingShingle | Hiroshi Ichikawa Tatsuo Kanda Shin-ichi Kosugi Yasuyuki Kawachi Toshifumi Wakai Tadashi Kondo Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer EuPA Open Proteomics Gastric cancer Lymph node metastasis Label-free proteomics Beta-3 integrin Transcriptome |
| title | Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer |
| title_full | Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer |
| title_fullStr | Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer |
| title_full_unstemmed | Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer |
| title_short | Proteomic and meta-transcriptomic study on lymph node metastasis in gastric cancer |
| title_sort | proteomic and meta transcriptomic study on lymph node metastasis in gastric cancer |
| topic | Gastric cancer Lymph node metastasis Label-free proteomics Beta-3 integrin Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2212968514000208 |
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