Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
Abstract Objective This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. Results We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma sp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3455-x |
| _version_ | 1819123941396971520 |
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| author | Ira M. Sigar Justin H. Schripsema Kathleen A. Kelly Ashlesh K. Murthy Srikanth Manam Kyle H. Ramsey |
| author_facet | Ira M. Sigar Justin H. Schripsema Kathleen A. Kelly Ashlesh K. Murthy Srikanth Manam Kyle H. Ramsey |
| author_sort | Ira M. Sigar |
| collection | DOAJ |
| description | Abstract Objective This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. Results We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay. |
| first_indexed | 2024-12-22T07:16:21Z |
| format | Article |
| id | doaj.art-4ce781beb0554778999f6debfeb5245b |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-22T07:16:21Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-4ce781beb0554778999f6debfeb5245b2022-12-21T18:34:23ZengBMCBMC Research Notes1756-05002018-06-011111510.1186/s13104-018-3455-xElimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolationIra M. Sigar0Justin H. Schripsema1Kathleen A. Kelly2Ashlesh K. Murthy3Srikanth Manam4Kyle H. Ramsey5Microbiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern UniversityMicrobiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern UniversityPathology and Laboratory Medicine, David Geffen School of Medicine, at UCLACollege of Veterinary Medicine, Midwestern UniversityCollege of Veterinary Medicine, Midwestern UniversityMicrobiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern UniversityAbstract Objective This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. Results We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.http://link.springer.com/article/10.1186/s13104-018-3455-xChlamydiaMycoplasmaCell cultureContaminationPlaque assay |
| spellingShingle | Ira M. Sigar Justin H. Schripsema Kathleen A. Kelly Ashlesh K. Murthy Srikanth Manam Kyle H. Ramsey Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation BMC Research Notes Chlamydia Mycoplasma Cell culture Contamination Plaque assay |
| title | Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation |
| title_full | Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation |
| title_fullStr | Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation |
| title_full_unstemmed | Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation |
| title_short | Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation |
| title_sort | elimination of mycoplasma contamination in chlamydia stocks as a result of in vivo passage or plaque isolation |
| topic | Chlamydia Mycoplasma Cell culture Contamination Plaque assay |
| url | http://link.springer.com/article/10.1186/s13104-018-3455-x |
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