Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species
Purpose: Fusarium keratitis is a severe infection of the anterior eye, frequently leading to keratoplasty or surgical removal of the affected eye. A major risk factor for infection is the use of contact lenses. Inadequate hygiene precautions and mold-growth permissive storage fluids are important ri...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | International Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1438422124000067 |
| _version_ | 1797256408642617344 |
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| author | Benedikt Schrenker Anna Zimmermann Thorsten Koch Grit Walther Ronny Martin Daniel Kampik Oliver Kurzai Johanna Theuersbacher |
| author_facet | Benedikt Schrenker Anna Zimmermann Thorsten Koch Grit Walther Ronny Martin Daniel Kampik Oliver Kurzai Johanna Theuersbacher |
| author_sort | Benedikt Schrenker |
| collection | DOAJ |
| description | Purpose: Fusarium keratitis is a severe infection of the anterior eye, frequently leading to keratoplasty or surgical removal of the affected eye. A major risk factor for infection is the use of contact lenses. Inadequate hygiene precautions and mold-growth permissive storage fluids are important risk factors for fungal keratitis. The aim of this study was to comparatively analyze contact lens storage fluids disinfection efficacy against Fusarium species. Methods: Eleven commercially available storage fluids were tested. The storage fluids were classified according to their active ingredients myristamidopropyldimethylamine (Aldox), polyhexanide and hydrogen peroxide. Efficacy was tested against isolates belonging to the Fusarium solani and Fusarium oxysporum species complexes as the most common agents of mould keratitis. Tests were carried out based on DIN EN ISO 14729. Results: All Aldox and hydrogen peroxide (H2O2) based fluids were effective against Fusarium spp., while the majority of polyhexanide based storage fluids showed only limited or no antifungal effects. Efficacy of polyhexanide could be restored by the addition of the pH-regulating agent tromethamine - an additive component in one commercially available product. Conclusions: In summary, the use of Aldox- or hydrogen peroxide-based storage fluids may reduce the risk of Fusarium keratitis, while polyhexanide-based agents largely lack efficacy against Fusarium. |
| first_indexed | 2024-03-08T10:30:22Z |
| format | Article |
| id | doaj.art-4ceb52f3444e4916b1e424728ef9a415 |
| institution | Directory Open Access Journal |
| issn | 1438-4221 |
| language | English |
| last_indexed | 2024-04-24T22:21:16Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Medical Microbiology |
| spelling | doaj.art-4ceb52f3444e4916b1e424728ef9a4152024-03-20T06:08:45ZengElsevierInternational Journal of Medical Microbiology1438-42212024-03-01314151602Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium speciesBenedikt Schrenker0Anna Zimmermann1Thorsten Koch2Grit Walther3Ronny Martin4Daniel Kampik5Oliver Kurzai6Johanna Theuersbacher7Institute for Hygiene and Microbiology, University of Würzburg, Germany; Department of Ophthalmology, University Hospital Würzburg, GermanyInstitute for Hygiene and Microbiology, University of Würzburg, GermanyInstitute for Hygiene and Microbiology, University of Würzburg, GermanyNational Reference Center for Invasive Fungal Infections NRZMyk, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Jena, GermanyInstitute for Hygiene and Microbiology, University of Würzburg, GermanyDepartment of Ophthalmology, University Hospital Würzburg, GermanyInstitute for Hygiene and Microbiology, University of Würzburg, Germany; National Reference Center for Invasive Fungal Infections NRZMyk, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Jena, Germany; Correspondence to: Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Straße 2, E1, Würzburg 97080, Germany.Department of Ophthalmology, University Hospital Würzburg, GermanyPurpose: Fusarium keratitis is a severe infection of the anterior eye, frequently leading to keratoplasty or surgical removal of the affected eye. A major risk factor for infection is the use of contact lenses. Inadequate hygiene precautions and mold-growth permissive storage fluids are important risk factors for fungal keratitis. The aim of this study was to comparatively analyze contact lens storage fluids disinfection efficacy against Fusarium species. Methods: Eleven commercially available storage fluids were tested. The storage fluids were classified according to their active ingredients myristamidopropyldimethylamine (Aldox), polyhexanide and hydrogen peroxide. Efficacy was tested against isolates belonging to the Fusarium solani and Fusarium oxysporum species complexes as the most common agents of mould keratitis. Tests were carried out based on DIN EN ISO 14729. Results: All Aldox and hydrogen peroxide (H2O2) based fluids were effective against Fusarium spp., while the majority of polyhexanide based storage fluids showed only limited or no antifungal effects. Efficacy of polyhexanide could be restored by the addition of the pH-regulating agent tromethamine - an additive component in one commercially available product. Conclusions: In summary, the use of Aldox- or hydrogen peroxide-based storage fluids may reduce the risk of Fusarium keratitis, while polyhexanide-based agents largely lack efficacy against Fusarium.http://www.sciencedirect.com/science/article/pii/S1438422124000067PolyhexanideContact lens storage fluidFusariumDIN EN ISO 14729Keratitis |
| spellingShingle | Benedikt Schrenker Anna Zimmermann Thorsten Koch Grit Walther Ronny Martin Daniel Kampik Oliver Kurzai Johanna Theuersbacher Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species International Journal of Medical Microbiology Polyhexanide Contact lens storage fluid Fusarium DIN EN ISO 14729 Keratitis |
| title | Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species |
| title_full | Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species |
| title_fullStr | Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species |
| title_full_unstemmed | Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species |
| title_short | Polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against Fusarium species |
| title_sort | polyhexanide based contact lens storage fluids frequently exhibit insufficient antifungal activity against fusarium species |
| topic | Polyhexanide Contact lens storage fluid Fusarium DIN EN ISO 14729 Keratitis |
| url | http://www.sciencedirect.com/science/article/pii/S1438422124000067 |
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