Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS
In the current work, different studies were carried out on the lipase enzyme produced by the halotolerant Oceanobacillus iheyensis strain QCS, with an expectation to be an important candidate in the industrial applications. Lipase of strain QCS was halo-alkali-thermo-detergent-solvent stable. Maxi...
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| Format: | Article |
| Language: | English |
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The National Information and Documentation Centre (NIDOC) , Egypt
2020-08-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_107542_d6cbb961df5b2a1056d24d361b3b2e40.pdf |
| _version_ | 1797204315786444800 |
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| author | Noura Sh. A. Hagaggi |
| author_facet | Noura Sh. A. Hagaggi |
| author_sort | Noura Sh. A. Hagaggi |
| collection | DOAJ |
| description | In the current work, different studies were carried out on the lipase enzyme produced by the halotolerant
Oceanobacillus iheyensis strain QCS, with an expectation to be an important candidate in the industrial
applications. Lipase of strain QCS was halo-alkali-thermo-detergent-solvent stable. Maximum production of
lipase was obtained after 72 h of incubation, at 40oC and pH 8 and 9 in a medium containing 25 % (w/v) NaCl and
1% (v/v) olive oil as a lipid substrate. This lipase was partially purified, highest lipase activity was obtained in
80% ammonium sulfate saturation, and in fraction eight of the Sephadex G-200 gel filtration chromatography.
Lipase displayed wide spectrum of activity within a broad range of conditions including salinity, temperature and
pH, it was optimally active at 25% (w/v) NaCl, 40°C and pH 8 and 9, respectively. The effect of many metal ions,
detergents and organic solvents on the activity of lipase was evaluated. Interestingly, lipase was able to retain the
majority of its activity in the presence of Ni2+, Mg2+, Oxi and Fairy detergents, ethyl acetate, dimethyl formamide
and toluene, respectively. Overall, as the lipase from O. iheyensis strain QCS has a number of interesting
properties especially its stability at extreme conditions; it could be used as a potential promising candidate for
detergents industry, and as a biocatalyst in low water enzymatic processes. |
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| format | Article |
| id | doaj.art-4cf0ce3a47fe4364a3aa219855fec8c7 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:33:17Z |
| publishDate | 2020-08-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-4cf0ce3a47fe4364a3aa219855fec8c72024-04-16T18:17:38ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942020-08-014490792010.21608/nrmj.2020.107542Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCSNoura Sh. A. Hagaggi0Botany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptIn the current work, different studies were carried out on the lipase enzyme produced by the halotolerant Oceanobacillus iheyensis strain QCS, with an expectation to be an important candidate in the industrial applications. Lipase of strain QCS was halo-alkali-thermo-detergent-solvent stable. Maximum production of lipase was obtained after 72 h of incubation, at 40oC and pH 8 and 9 in a medium containing 25 % (w/v) NaCl and 1% (v/v) olive oil as a lipid substrate. This lipase was partially purified, highest lipase activity was obtained in 80% ammonium sulfate saturation, and in fraction eight of the Sephadex G-200 gel filtration chromatography. Lipase displayed wide spectrum of activity within a broad range of conditions including salinity, temperature and pH, it was optimally active at 25% (w/v) NaCl, 40°C and pH 8 and 9, respectively. The effect of many metal ions, detergents and organic solvents on the activity of lipase was evaluated. Interestingly, lipase was able to retain the majority of its activity in the presence of Ni2+, Mg2+, Oxi and Fairy detergents, ethyl acetate, dimethyl formamide and toluene, respectively. Overall, as the lipase from O. iheyensis strain QCS has a number of interesting properties especially its stability at extreme conditions; it could be used as a potential promising candidate for detergents industry, and as a biocatalyst in low water enzymatic processes.https://nrmj.journals.ekb.eg/article_107542_d6cbb961df5b2a1056d24d361b3b2e40.pdflipaseoceanobacillus iheyensisextreme conditionshalotolerant |
| spellingShingle | Noura Sh. A. Hagaggi Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS Novel Research in Microbiology Journal lipase oceanobacillus iheyensis extreme conditions halotolerant |
| title | Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS |
| title_full | Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS |
| title_fullStr | Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS |
| title_full_unstemmed | Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS |
| title_short | Studies on the extremo-lipase produced by the halotolerant Oceanobacillus iheyensis strain QCS |
| title_sort | studies on the extremo lipase produced by the halotolerant oceanobacillus iheyensis strain qcs |
| topic | lipase oceanobacillus iheyensis extreme conditions halotolerant |
| url | https://nrmj.journals.ekb.eg/article_107542_d6cbb961df5b2a1056d24d361b3b2e40.pdf |
| work_keys_str_mv | AT nourashahagaggi studiesontheextremolipaseproducedbythehalotolerantoceanobacillusiheyensisstrainqcs |