HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis
Abstract Objective To study the effects of human herpes virus 6 (HHV‐6) on the hippocampal volume in patients with mesial temporal sclerosis (MTS). Background HHV‐6 may play an etiologic role in MTS. Previous studies found a possible association with febrile status epilepticus. Several investigators...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-09-01
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| Series: | Annals of Clinical and Translational Neurology |
| Online Access: | https://doi.org/10.1002/acn3.51152 |
| _version_ | 1818848610073182208 |
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| author | Elizabeth O. Akinsoji Emily Leibovitch B. Jeanne Billioux Osorio Lopes Abath Neto Abhik Ray‐Chaudhury Sara K. Inati Kareem Zaghloul John Heiss Steven Jacobson William H. Theodore |
| author_facet | Elizabeth O. Akinsoji Emily Leibovitch B. Jeanne Billioux Osorio Lopes Abath Neto Abhik Ray‐Chaudhury Sara K. Inati Kareem Zaghloul John Heiss Steven Jacobson William H. Theodore |
| author_sort | Elizabeth O. Akinsoji |
| collection | DOAJ |
| description | Abstract Objective To study the effects of human herpes virus 6 (HHV‐6) on the hippocampal volume in patients with mesial temporal sclerosis (MTS). Background HHV‐6 may play an etiologic role in MTS. Previous studies found a possible association with febrile status epilepticus. Several investigators have reported a higher prevalence of HHV‐6 in MTS resections compared to other epilepsy etiologies. Design/Methods We used FreeSurfer to segment cortical structures and obtain whole hippocampal and subfield volumes in 41 patients with intractable epilepsy. In addition, an investigator blinded to other data traced hippocampi manually on each slice. The main study outcome measure was the asymmetry index (AI) between hippocampal volumes ipsilateral and contralateral to seizure foci compared between HHV‐6 positive and negative patients. Viral DNA was isolated from fresh brain tissue obtained at temporal lobectomy. For 25 patients, viral detection was performed using quantitative real‐time PCR specific for HHV‐6A and HHV‐6B. For 16 patients, viral DNA detection was performed using digital droplet PCR specific for HHV‐6A and HHV‐6B. Results Twenty‐two patients were positive (14 of 25 tested with real‐time PCR, and 8 of 16 with digital droplet PCR), and 19 negatives for HHV‐6. HHV‐6 negative patients had significantly greater AI and lower total hippocampal volume ipsilateral to seizure foci than HHV‐6 positive patients. Epilepsy duration and age of onset did not affect results. Interpretation Our data suggest multiple potential etiologies for MTS. HHV‐6 may have a less severe effect on the hippocampus than other etiologies. |
| first_indexed | 2024-12-19T06:20:04Z |
| format | Article |
| id | doaj.art-4cf510b6f0854f2e93926ce9c48216c4 |
| institution | Directory Open Access Journal |
| issn | 2328-9503 |
| language | English |
| last_indexed | 2024-12-19T06:20:04Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Annals of Clinical and Translational Neurology |
| spelling | doaj.art-4cf510b6f0854f2e93926ce9c48216c42022-12-21T20:32:45ZengWileyAnnals of Clinical and Translational Neurology2328-95032020-09-01791674168010.1002/acn3.51152HHV‐6 and hippocampal volume in patients with mesial temporal sclerosisElizabeth O. Akinsoji0Emily Leibovitch1B. Jeanne Billioux2Osorio Lopes Abath Neto3Abhik Ray‐Chaudhury4Sara K. Inati5Kareem Zaghloul6John Heiss7Steven Jacobson8William H. Theodore9Clinical Epilepsy Section National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandNeuroimmunology Branch National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandNeuroimmunology Branch National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandNational Institute of Neurological Disorders and Stroke National Cancer Institute NIH Bethesda MarylandSurgical Neurology Branch National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandEEG Laboratory National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandSurgical Neurology Branch National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandSurgical Neurology Branch National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandNeuroimmunology Branch National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandClinical Epilepsy Section National Institute of Neurological Disorders and Stroke NIH Bethesda MarylandAbstract Objective To study the effects of human herpes virus 6 (HHV‐6) on the hippocampal volume in patients with mesial temporal sclerosis (MTS). Background HHV‐6 may play an etiologic role in MTS. Previous studies found a possible association with febrile status epilepticus. Several investigators have reported a higher prevalence of HHV‐6 in MTS resections compared to other epilepsy etiologies. Design/Methods We used FreeSurfer to segment cortical structures and obtain whole hippocampal and subfield volumes in 41 patients with intractable epilepsy. In addition, an investigator blinded to other data traced hippocampi manually on each slice. The main study outcome measure was the asymmetry index (AI) between hippocampal volumes ipsilateral and contralateral to seizure foci compared between HHV‐6 positive and negative patients. Viral DNA was isolated from fresh brain tissue obtained at temporal lobectomy. For 25 patients, viral detection was performed using quantitative real‐time PCR specific for HHV‐6A and HHV‐6B. For 16 patients, viral DNA detection was performed using digital droplet PCR specific for HHV‐6A and HHV‐6B. Results Twenty‐two patients were positive (14 of 25 tested with real‐time PCR, and 8 of 16 with digital droplet PCR), and 19 negatives for HHV‐6. HHV‐6 negative patients had significantly greater AI and lower total hippocampal volume ipsilateral to seizure foci than HHV‐6 positive patients. Epilepsy duration and age of onset did not affect results. Interpretation Our data suggest multiple potential etiologies for MTS. HHV‐6 may have a less severe effect on the hippocampus than other etiologies.https://doi.org/10.1002/acn3.51152 |
| spellingShingle | Elizabeth O. Akinsoji Emily Leibovitch B. Jeanne Billioux Osorio Lopes Abath Neto Abhik Ray‐Chaudhury Sara K. Inati Kareem Zaghloul John Heiss Steven Jacobson William H. Theodore HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis Annals of Clinical and Translational Neurology |
| title | HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis |
| title_full | HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis |
| title_fullStr | HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis |
| title_full_unstemmed | HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis |
| title_short | HHV‐6 and hippocampal volume in patients with mesial temporal sclerosis |
| title_sort | hhv 6 and hippocampal volume in patients with mesial temporal sclerosis |
| url | https://doi.org/10.1002/acn3.51152 |
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