Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway
Background and aim: Triple-negative breast cancer (TNBC) is a highly invasive type of breast cancer with a poor prognosis. Currently, there are no effective management strategies for TNBC. Earlier, our lab reported the percolation of Spatholobus suberectus for the treatment of breast cancer. Lipid m...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411023000998 |
| _version_ | 1797643592677720064 |
|---|---|
| author | Xiaohui Zeng Guowei Gong Kumar Ganesan Yi Wen Qingqing Liu Juncheng Zhuo Jianming Wu Jianping Chen |
| author_facet | Xiaohui Zeng Guowei Gong Kumar Ganesan Yi Wen Qingqing Liu Juncheng Zhuo Jianming Wu Jianping Chen |
| author_sort | Xiaohui Zeng |
| collection | DOAJ |
| description | Background and aim: Triple-negative breast cancer (TNBC) is a highly invasive type of breast cancer with a poor prognosis. Currently, there are no effective management strategies for TNBC. Earlier, our lab reported the percolation of Spatholobus suberectus for the treatment of breast cancer. Lipid metabolic reprogramming is a hallmark of cancer. However, the anti-TNBC efficiency of S. suberectus extract and its causal mechanism for preventing lipogenesis have not been fully recognized. Hence, the present study aimed to investigate the inhibitory role of S. suberectus extract on lipogenesis and tumorigenesis in TNBC in vitro and in vivo by activating AMPK-ACC and K-Ras-ERK signaling pathways using lipidomic and metabolomic techniques. Experimental procedure: Dried stems of S. suberectus extract inhibited lipogenesis and tumorigenesis and promoted fatty acid oxidation as demonstrated by the identification of the metabolites and fatty acid markers using proteomic and metabolomic analysis, qPCR, and Western blot. Results and conclusion: The results indicated that S. suberectus extract promotes fatty acid oxidation and suppresses lipogenic metabolites and biomarkers, thereby preventing tumorigenesis via the AMPK-ACC and K-Ras-ERK signaling pathways. On the basis of this preclinical evidence, we suggest that this study represents a milestone and complements Chinese medicine. Further studies remain underway in our laboratory to elucidate the active principles of S. suberectus extract. This study suggests that S. suberectus extract could be a promising therapy for TNBC. |
| first_indexed | 2024-03-11T14:17:07Z |
| format | Article |
| id | doaj.art-4cf57f7c649c4bc69a7216da20125bb6 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-03-11T14:17:07Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-4cf57f7c649c4bc69a7216da20125bb62023-11-01T04:47:20ZengElsevierJournal of Traditional and Complementary Medicine2225-41102023-11-01136623638Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathwayXiaohui Zeng0Guowei Gong1Kumar Ganesan2Yi Wen3Qingqing Liu4Juncheng Zhuo5Jianming Wu6Jianping Chen7School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine, Guangzhou, Guangdong, 510095, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, China; Zhongshan People's Hospital, 106, Zhongshan 2nd Road, Guangdong Province, 510080, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, China; Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, 518000, ChinaGuangdong Second Traditional Chinese Medicine Hospital, Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine, Guangzhou, Guangdong, 510095, ChinaSchool of Pharmacy, Southwest Medical University, Luzhou, 646000, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, China; Shenzhen Institute of Research and Innovation, The University of Hong Kong, Shenzhen, 518000, China; Corresponding author. School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Sassoon Road, Pokfulam, Hong Kong, China.Background and aim: Triple-negative breast cancer (TNBC) is a highly invasive type of breast cancer with a poor prognosis. Currently, there are no effective management strategies for TNBC. Earlier, our lab reported the percolation of Spatholobus suberectus for the treatment of breast cancer. Lipid metabolic reprogramming is a hallmark of cancer. However, the anti-TNBC efficiency of S. suberectus extract and its causal mechanism for preventing lipogenesis have not been fully recognized. Hence, the present study aimed to investigate the inhibitory role of S. suberectus extract on lipogenesis and tumorigenesis in TNBC in vitro and in vivo by activating AMPK-ACC and K-Ras-ERK signaling pathways using lipidomic and metabolomic techniques. Experimental procedure: Dried stems of S. suberectus extract inhibited lipogenesis and tumorigenesis and promoted fatty acid oxidation as demonstrated by the identification of the metabolites and fatty acid markers using proteomic and metabolomic analysis, qPCR, and Western blot. Results and conclusion: The results indicated that S. suberectus extract promotes fatty acid oxidation and suppresses lipogenic metabolites and biomarkers, thereby preventing tumorigenesis via the AMPK-ACC and K-Ras-ERK signaling pathways. On the basis of this preclinical evidence, we suggest that this study represents a milestone and complements Chinese medicine. Further studies remain underway in our laboratory to elucidate the active principles of S. suberectus extract. This study suggests that S. suberectus extract could be a promising therapy for TNBC.http://www.sciencedirect.com/science/article/pii/S2225411023000998Spatholobi suberectusLipogenesisTNBCTherapyNatural productProteomic and metabolomic analysis |
| spellingShingle | Xiaohui Zeng Guowei Gong Kumar Ganesan Yi Wen Qingqing Liu Juncheng Zhuo Jianming Wu Jianping Chen Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway Journal of Traditional and Complementary Medicine Spatholobi suberectus Lipogenesis TNBC Therapy Natural product Proteomic and metabolomic analysis |
| title | Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway |
| title_full | Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway |
| title_fullStr | Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway |
| title_full_unstemmed | Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway |
| title_short | Spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple-negative breast cancer via activation of AMPK-ACC and K-Ras-ERK signaling pathway |
| title_sort | spatholobus suberectus inhibits lipogenesis and tumorigenesis in triple negative breast cancer via activation of ampk acc and k ras erk signaling pathway |
| topic | Spatholobi suberectus Lipogenesis TNBC Therapy Natural product Proteomic and metabolomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2225411023000998 |
| work_keys_str_mv | AT xiaohuizeng spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT guoweigong spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT kumarganesan spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT yiwen spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT qingqingliu spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT junchengzhuo spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT jianmingwu spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway AT jianpingchen spatholobussuberectusinhibitslipogenesisandtumorigenesisintriplenegativebreastcancerviaactivationofampkaccandkraserksignalingpathway |