Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue
A high-throughput positive-selection approach was taken to generate a dataset of Shigatoxigenic Escherichia coli (STEC) O157:H7 genes enriched in adherence to plant tissue. The approach generates a differential dataset based on BAC clones enriched in the output, after adherence, compared to the inoc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340920306636 |
| _version_ | 1818292135198195712 |
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| author | Ashleigh Holmes Leighton Pritchard Peter Hedley Jenny Morris Sean P. McAteer David L. Gally Nicola J. Holden |
| author_facet | Ashleigh Holmes Leighton Pritchard Peter Hedley Jenny Morris Sean P. McAteer David L. Gally Nicola J. Holden |
| author_sort | Ashleigh Holmes |
| collection | DOAJ |
| description | A high-throughput positive-selection approach was taken to generate a dataset of Shigatoxigenic Escherichia coli (STEC) O157:H7 genes enriched in adherence to plant tissue. The approach generates a differential dataset based on BAC clones enriched in the output, after adherence, compared to the inoculum used as the input. A BAC clone library derived from STEC isolate ‘Sakai’ was used since this isolate is associated with a very large-scale outbreak of human disease from consumption of contaminated fresh produce; white radish sprouts. Spinach was used for the screen since it is associated with STEC outbreaks, and the roots provide a suitable site for bacterial colonisation. Four successive of rounds of Sakai BAC clone selection and amplification were applied for spinach root adherence, in parallel to a non-plant control. Genomic DNA was obtained from a total of 7.17 × 108 cfu/ml of bacteria from the plant treatment and 1.13 × 109 cfu/ml of bacteria from the no-plant control. Relative gene abundance of the output compared to the input pools was obtained using an established E. coli DNA microarray chip for STEC. The dataset enables screening for genes enriched under the treatment condition and informs on genes that may play a role in plant-microbe interactions. |
| first_indexed | 2024-12-13T02:55:09Z |
| format | Article |
| id | doaj.art-4cf6465c729c4f78abd5dd1bf24b2f77 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-13T02:55:09Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-4cf6465c729c4f78abd5dd1bf24b2f772022-12-22T00:01:59ZengElsevierData in Brief2352-34092020-08-0131105769Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissueAshleigh Holmes0Leighton Pritchard1Peter Hedley2Jenny Morris3Sean P. McAteer4David L. Gally5Nicola J. Holden6Cellular and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, United KingdomCellular and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, United Kingdom; Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, United KingdomCellular and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, United KingdomCellular and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, United KingdomThe Roslin Institute Division of Infection and Immunity, University of Edinburgh, R(D)SVS, The Roslin Institute Building, Easter Bush EH25 9RG, United KingdomThe Roslin Institute Division of Infection and Immunity, University of Edinburgh, R(D)SVS, The Roslin Institute Building, Easter Bush EH25 9RG, United KingdomCellular and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, United Kingdom; Corresponding author.A high-throughput positive-selection approach was taken to generate a dataset of Shigatoxigenic Escherichia coli (STEC) O157:H7 genes enriched in adherence to plant tissue. The approach generates a differential dataset based on BAC clones enriched in the output, after adherence, compared to the inoculum used as the input. A BAC clone library derived from STEC isolate ‘Sakai’ was used since this isolate is associated with a very large-scale outbreak of human disease from consumption of contaminated fresh produce; white radish sprouts. Spinach was used for the screen since it is associated with STEC outbreaks, and the roots provide a suitable site for bacterial colonisation. Four successive of rounds of Sakai BAC clone selection and amplification were applied for spinach root adherence, in parallel to a non-plant control. Genomic DNA was obtained from a total of 7.17 × 108 cfu/ml of bacteria from the plant treatment and 1.13 × 109 cfu/ml of bacteria from the no-plant control. Relative gene abundance of the output compared to the input pools was obtained using an established E. coli DNA microarray chip for STEC. The dataset enables screening for genes enriched under the treatment condition and informs on genes that may play a role in plant-microbe interactions.http://www.sciencedirect.com/science/article/pii/S2352340920306636MicroarrayBacterial artificial chromosomeGene enrichmentHigh-throughput screeningBacteria-host interactions |
| spellingShingle | Ashleigh Holmes Leighton Pritchard Peter Hedley Jenny Morris Sean P. McAteer David L. Gally Nicola J. Holden Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue Data in Brief Microarray Bacterial artificial chromosome Gene enrichment High-throughput screening Bacteria-host interactions |
| title | Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue |
| title_full | Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue |
| title_fullStr | Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue |
| title_full_unstemmed | Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue |
| title_short | Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue |
| title_sort | dataset of escherichia coli o157 h7 genes enriched in adherence to spinach root tissue |
| topic | Microarray Bacterial artificial chromosome Gene enrichment High-throughput screening Bacteria-host interactions |
| url | http://www.sciencedirect.com/science/article/pii/S2352340920306636 |
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