Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756
Effects of physical and chemical treatment on nutrient mobility, their utilization for menaquinone-7 (MK-7) biosynthesis; growth of microbial cells has been investigated in the present research. Bacillus subtilis MTCC 2756 fermented medium was supplied with 1-naphthol and hypoxanthine resulted in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2015-06-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/37-3/37-3-6.pdf |
| _version_ | 1811219692145082368 |
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| author | Alka Puri Murteza Iqubal Rasheeduz Zafar Bibhu Prasad Panda |
| author_facet | Alka Puri Murteza Iqubal Rasheeduz Zafar Bibhu Prasad Panda |
| author_sort | Alka Puri |
| collection | DOAJ |
| description | Effects of physical and chemical treatment on nutrient mobility, their utilization for menaquinone-7 (MK-7) biosynthesis;
growth of microbial cells has been investigated in the present research. Bacillus subtilis MTCC 2756 fermented medium was
supplied with 1-naphthol and hypoxanthine resulted in a significant increase in MK-7 production. Ultrasonication, electric
shock, heat shock, and tween 80 were used for inducer uptake by Bacillus subtilis and menaquinone-7 production. Induction
of Bacillus subtilis (at 16 hours of fermentation) using 1-naphthol (2 mg/ml), along with tween 80 (0.1%) was found to increase
the MK-7 production by 3 fold i.e. 14.4 µg/ml as compared to the untreated fermentation medium. The ultrasonicated (ultrasonic
power 33 W, treatment time 4 min and frequency 36 KHz) microbial cells yielded higher biomass and 2.5 fold increase
in the MK-7 production i.e.10.3 µg/ml than control. 1-naphthol along with physical or chemical treatment is required for
maximum MK-7 production by Bacillus subtilis. |
| first_indexed | 2024-04-12T07:30:13Z |
| format | Article |
| id | doaj.art-4cf6f977c41947e69edbcec094cf80f7 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-12T07:30:13Z |
| publishDate | 2015-06-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-4cf6f977c41947e69edbcec094cf80f72022-12-22T03:42:05ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952015-06-01373283289Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756Alka Puri0Murteza Iqubal1Rasheeduz Zafar2Bibhu Prasad Panda3Microbial and Pharmaceutical Biotechnology Laboratory, Centre of Advance Research in Pharmaceutical Sciences, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, IndiaMicrobial and Pharmaceutical Biotechnology Laboratory, Centre of Advance Research in Pharmaceutical Sciences, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, IndiaMicrobial and Pharmaceutical Biotechnology Laboratory, Centre of Advance Research in Pharmaceutical Sciences, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, IndiaMicrobial and Pharmaceutical Biotechnology Laboratory, Centre of Advance Research in Pharmaceutical Sciences, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, IndiaEffects of physical and chemical treatment on nutrient mobility, their utilization for menaquinone-7 (MK-7) biosynthesis; growth of microbial cells has been investigated in the present research. Bacillus subtilis MTCC 2756 fermented medium was supplied with 1-naphthol and hypoxanthine resulted in a significant increase in MK-7 production. Ultrasonication, electric shock, heat shock, and tween 80 were used for inducer uptake by Bacillus subtilis and menaquinone-7 production. Induction of Bacillus subtilis (at 16 hours of fermentation) using 1-naphthol (2 mg/ml), along with tween 80 (0.1%) was found to increase the MK-7 production by 3 fold i.e. 14.4 µg/ml as compared to the untreated fermentation medium. The ultrasonicated (ultrasonic power 33 W, treatment time 4 min and frequency 36 KHz) microbial cells yielded higher biomass and 2.5 fold increase in the MK-7 production i.e.10.3 µg/ml than control. 1-naphthol along with physical or chemical treatment is required for maximum MK-7 production by Bacillus subtilis.http://rdo.psu.ac.th/sjstweb/journal/37-3/37-3-6.pdfmenaquinone-71-naphtholhypoxanthineultrasonicationtween 80 |
| spellingShingle | Alka Puri Murteza Iqubal Rasheeduz Zafar Bibhu Prasad Panda Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756 Songklanakarin Journal of Science and Technology (SJST) menaquinone-7 1-naphthol hypoxanthine ultrasonication tween 80 |
| title | Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756 |
| title_full | Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756 |
| title_fullStr | Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756 |
| title_full_unstemmed | Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756 |
| title_short | Influence of physical, chemical and inducer treatments on menaquinone-7 biosynthesis by Bacillus subtilis MTCC 2756 |
| title_sort | influence of physical chemical and inducer treatments on menaquinone 7 biosynthesis by bacillus subtilis mtcc 2756 |
| topic | menaquinone-7 1-naphthol hypoxanthine ultrasonication tween 80 |
| url | http://rdo.psu.ac.th/sjstweb/journal/37-3/37-3-6.pdf |
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