Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae)
Abstract Background Standardized larval rearing units for mosquito production are essential for the establishment of a mass-rearing facility. Two larval rearing units, developed respectively by the Guangzhou Wolbaki Biotech Co. Ltd. (Wolbaki) and Insect Pest Control Laboratory, Joint FAO/IAEA Divisi...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2511-z |
| _version_ | 1819069735389626368 |
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| author | Dongjing Zhang Meichun Zhang Yu Wu Jeremie R. L. Gilles Hanano Yamada Zhongdao Wu Zhiyong Xi Xiaoying Zheng |
| author_facet | Dongjing Zhang Meichun Zhang Yu Wu Jeremie R. L. Gilles Hanano Yamada Zhongdao Wu Zhiyong Xi Xiaoying Zheng |
| author_sort | Dongjing Zhang |
| collection | DOAJ |
| description | Abstract Background Standardized larval rearing units for mosquito production are essential for the establishment of a mass-rearing facility. Two larval rearing units, developed respectively by the Guangzhou Wolbaki Biotech Co. Ltd. (Wolbaki) and Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture (FAO/IAEA-IPCL), are tested to assess their potential uses to mass-rear the larval stages of Aedes albopictus in support of the establishment of a medium-scale mosquito facility for the application of mosquito genetic control strategies. Methods The triple Wolbachia-infected Ae. albopictus strain (HC strain) was used in this study. The effects of larval densities of two larval rearing trays (corresponding to 2.4, 3.0 and 3.6 larvae/cm2) and tray size/position (top, middle and bottom layers) on the pupae production and larval survival were assessed when trays were stacked within the larval rearing units. The male pupae production, female pupae contamination after sex separation, and male mating competitiveness were also studied by using both larval rearing units in their entirety. Results The optimal larval rearing density for Wolbaki-tray (Wol-tray) was 6,600 larvae (equal to 3.0 larvae/cm2) and 18,000 larvae (3.6 larvae/cm2) for the FAO/IAEA-IPCL tray (IAEA-tray). No significant difference in pupae production was observed when trays were stacked within top, middle or bottom layers for both units. At thirty-four hours after the first pupation, the average male pupae production was (0.89 × 105) for the Wol-unit and (3.16 × 105) for the IAEA-unit. No significant difference was observed in female pupae contamination between these two units. The HC males showed equal male mating competitiveness to wild type males for mating with wild type females in large cages, regardless of whether they were reared in the Wol-unit or IAEA-unit. Conclusions The current study has indicated that both the Wol-unit and IAEA-unit are suitable for larvae mass-rearing for Ae. albopictus. However, the IAEA-unit, with higher male production and less space required compared to the Wol-unit, is recommended to be used in support of the establishment of a medium-sized mosquito facility. |
| first_indexed | 2024-12-21T16:54:46Z |
| format | Article |
| id | doaj.art-4cf8cf754e57472ca96ccddb87e437ba |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-21T16:54:46Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-4cf8cf754e57472ca96ccddb87e437ba2022-12-21T18:56:47ZengBMCParasites & Vectors1756-33052017-11-011011910.1186/s13071-017-2511-zEstablishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae)Dongjing Zhang0Meichun Zhang1Yu Wu2Jeremie R. L. Gilles3Hanano Yamada4Zhongdao Wu5Zhiyong Xi6Xiaoying Zheng7Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen UniversitySun Yat-sen University - Michigan State University Joint Center of Vector Control for Tropical Diseases, Zhongshan School of MedicineDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-sen UniversityInsect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureInsect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-sen UniversitySun Yat-sen University - Michigan State University Joint Center of Vector Control for Tropical Diseases, Zhongshan School of MedicineDepartment of Parasitology, Zhongshan School of Medicine, Sun Yat-sen UniversityAbstract Background Standardized larval rearing units for mosquito production are essential for the establishment of a mass-rearing facility. Two larval rearing units, developed respectively by the Guangzhou Wolbaki Biotech Co. Ltd. (Wolbaki) and Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture (FAO/IAEA-IPCL), are tested to assess their potential uses to mass-rear the larval stages of Aedes albopictus in support of the establishment of a medium-scale mosquito facility for the application of mosquito genetic control strategies. Methods The triple Wolbachia-infected Ae. albopictus strain (HC strain) was used in this study. The effects of larval densities of two larval rearing trays (corresponding to 2.4, 3.0 and 3.6 larvae/cm2) and tray size/position (top, middle and bottom layers) on the pupae production and larval survival were assessed when trays were stacked within the larval rearing units. The male pupae production, female pupae contamination after sex separation, and male mating competitiveness were also studied by using both larval rearing units in their entirety. Results The optimal larval rearing density for Wolbaki-tray (Wol-tray) was 6,600 larvae (equal to 3.0 larvae/cm2) and 18,000 larvae (3.6 larvae/cm2) for the FAO/IAEA-IPCL tray (IAEA-tray). No significant difference in pupae production was observed when trays were stacked within top, middle or bottom layers for both units. At thirty-four hours after the first pupation, the average male pupae production was (0.89 × 105) for the Wol-unit and (3.16 × 105) for the IAEA-unit. No significant difference was observed in female pupae contamination between these two units. The HC males showed equal male mating competitiveness to wild type males for mating with wild type females in large cages, regardless of whether they were reared in the Wol-unit or IAEA-unit. Conclusions The current study has indicated that both the Wol-unit and IAEA-unit are suitable for larvae mass-rearing for Ae. albopictus. However, the IAEA-unit, with higher male production and less space required compared to the Wol-unit, is recommended to be used in support of the establishment of a medium-sized mosquito facility.http://link.springer.com/article/10.1186/s13071-017-2511-zMosquito facilityLarval rearing unitsMass-rearingAedes albopictus |
| spellingShingle | Dongjing Zhang Meichun Zhang Yu Wu Jeremie R. L. Gilles Hanano Yamada Zhongdao Wu Zhiyong Xi Xiaoying Zheng Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae) Parasites & Vectors Mosquito facility Larval rearing units Mass-rearing Aedes albopictus |
| title | Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae) |
| title_full | Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae) |
| title_fullStr | Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae) |
| title_full_unstemmed | Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae) |
| title_short | Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae) |
| title_sort | establishment of a medium scale mosquito facility optimization of the larval mass rearing unit for aedes albopictus diptera culicidae |
| topic | Mosquito facility Larval rearing units Mass-rearing Aedes albopictus |
| url | http://link.springer.com/article/10.1186/s13071-017-2511-z |
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