VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to assess the distribution of key SNARE proteins in glutamatergic and GABAergic synapses of the adult rat cerebellar cortex using light microscopy immunohistochemical techniques. Analysis was made of co-loca...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-11-01
|
| Series: | BMC Neuroscience |
| Online Access: | http://www.biomedcentral.com/1471-2202/12/118 |
| _version_ | 1818578812073410560 |
|---|---|
| author | Benagiano Vincenzo Lorusso Loredana Flace Paolo Girolamo Francesco Rizzi Anna Bosco Lorenzo Cagiano Raffaele Nico Beatrice Ribatti Domenico Ambrosi Glauco |
| author_facet | Benagiano Vincenzo Lorusso Loredana Flace Paolo Girolamo Francesco Rizzi Anna Bosco Lorenzo Cagiano Raffaele Nico Beatrice Ribatti Domenico Ambrosi Glauco |
| author_sort | Benagiano Vincenzo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The aim of this study was to assess the distribution of key SNARE proteins in glutamatergic and GABAergic synapses of the adult rat cerebellar cortex using light microscopy immunohistochemical techniques. Analysis was made of co-localizations of vGluT-1 and vGluT-2, vesicular transporters of glutamate and markers of glutamatergic synapses, or GAD, the GABA synthetic enzyme and marker of GABAergic synapses, with VAMP-2, SNAP-25A/B and syntaxin-1.</p> <p>Results</p> <p>The examined SNARE proteins were found to be diffusely expressed in glutamatergic synapses, whereas they were rarely observed in GABAergic synapses. However, among glutamatergic synapses, subpopulations which did not contain VAMP-2, SNAP-25A/B and syntaxin-1 were detected. They included virtually all the synapses established by terminals of climbing fibres (immunoreactive for vGluT-2) and some synapses established by terminals of parallel and mossy fibres (immunoreactive for vGluT-1, and for vGluT-1 and 2, respectively). The only GABA synapses expressing the SNARE proteins studied were the synapses established by axon terminals of basket neurons.</p> <p>Conclusion</p> <p>The present study supplies a detailed morphological description of VAMP-2, SNAP-25A/B and syntaxin-1 in the different types of glutamatergic and GABAergic synapses of the rat cerebellar cortex. The examined SNARE proteins characterize most of glutamatergic synapses and only one type of GABAergic synapses. In the subpopulations of glutamatergic and GABAergic synapses lacking the SNARE protein isoforms examined, alternative mechanisms for regulating trafficking of synaptic vesicles may be hypothesized, possibly mediated by different isoforms or homologous proteins.</p> |
| first_indexed | 2024-12-16T06:51:45Z |
| format | Article |
| id | doaj.art-4cfcf73440d24a699b367691618f89be |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-16T06:51:45Z |
| publishDate | 2011-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-4cfcf73440d24a699b367691618f89be2022-12-21T22:40:23ZengBMCBMC Neuroscience1471-22022011-11-0112111810.1186/1471-2202-12-118VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortexBenagiano VincenzoLorusso LoredanaFlace PaoloGirolamo FrancescoRizzi AnnaBosco LorenzoCagiano RaffaeleNico BeatriceRibatti DomenicoAmbrosi Glauco<p>Abstract</p> <p>Background</p> <p>The aim of this study was to assess the distribution of key SNARE proteins in glutamatergic and GABAergic synapses of the adult rat cerebellar cortex using light microscopy immunohistochemical techniques. Analysis was made of co-localizations of vGluT-1 and vGluT-2, vesicular transporters of glutamate and markers of glutamatergic synapses, or GAD, the GABA synthetic enzyme and marker of GABAergic synapses, with VAMP-2, SNAP-25A/B and syntaxin-1.</p> <p>Results</p> <p>The examined SNARE proteins were found to be diffusely expressed in glutamatergic synapses, whereas they were rarely observed in GABAergic synapses. However, among glutamatergic synapses, subpopulations which did not contain VAMP-2, SNAP-25A/B and syntaxin-1 were detected. They included virtually all the synapses established by terminals of climbing fibres (immunoreactive for vGluT-2) and some synapses established by terminals of parallel and mossy fibres (immunoreactive for vGluT-1, and for vGluT-1 and 2, respectively). The only GABA synapses expressing the SNARE proteins studied were the synapses established by axon terminals of basket neurons.</p> <p>Conclusion</p> <p>The present study supplies a detailed morphological description of VAMP-2, SNAP-25A/B and syntaxin-1 in the different types of glutamatergic and GABAergic synapses of the rat cerebellar cortex. The examined SNARE proteins characterize most of glutamatergic synapses and only one type of GABAergic synapses. In the subpopulations of glutamatergic and GABAergic synapses lacking the SNARE protein isoforms examined, alternative mechanisms for regulating trafficking of synaptic vesicles may be hypothesized, possibly mediated by different isoforms or homologous proteins.</p>http://www.biomedcentral.com/1471-2202/12/118 |
| spellingShingle | Benagiano Vincenzo Lorusso Loredana Flace Paolo Girolamo Francesco Rizzi Anna Bosco Lorenzo Cagiano Raffaele Nico Beatrice Ribatti Domenico Ambrosi Glauco VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex BMC Neuroscience |
| title | VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex |
| title_full | VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex |
| title_fullStr | VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex |
| title_full_unstemmed | VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex |
| title_short | VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex |
| title_sort | vamp 2 snap 25a b and syntaxin 1 in glutamatergic and gabaergic synapses of the rat cerebellar cortex |
| url | http://www.biomedcentral.com/1471-2202/12/118 |
| work_keys_str_mv | AT benagianovincenzo vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT lorussoloredana vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT flacepaolo vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT girolamofrancesco vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT rizzianna vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT boscolorenzo vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT cagianoraffaele vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT nicobeatrice vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT ribattidomenico vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex AT ambrosiglauco vamp2snap25abandsyntaxin1inglutamatergicandgabaergicsynapsesoftheratcerebellarcortex |