Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8
<i>Lactococcus lactis</i> is a commonly used fermenting bacteria in cheese, beverages and meat products. Due to the lack of simplified chassis strains, it has not been widely used in the fields of synthetic biology. Thus, the construction of lactic acid bacteria chassis strains becomes m...
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MDPI AG
2021-12-01
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| Series: | Microorganisms |
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| author | Wanjin Qiao Fulu Liu Xing Wan Yu Qiao Ran Li Zhenzhou Wu Per Erik Joakim Saris Haijin Xu Mingqiang Qiao |
| author_facet | Wanjin Qiao Fulu Liu Xing Wan Yu Qiao Ran Li Zhenzhou Wu Per Erik Joakim Saris Haijin Xu Mingqiang Qiao |
| author_sort | Wanjin Qiao |
| collection | DOAJ |
| description | <i>Lactococcus lactis</i> is a commonly used fermenting bacteria in cheese, beverages and meat products. Due to the lack of simplified chassis strains, it has not been widely used in the fields of synthetic biology. Thus, the construction of lactic acid bacteria chassis strains becomes more and more important. In this study, we performed whole genome sequencing, annotation and analysis of <i>L. lactis</i> N8. Based on the genome analysis, we found that <i>L. lactis</i> N8 contains two large plasmids, and the function prediction of the plasmids shows that some regions are related to carbohydrate transport/metabolism, multi-stress resistance and amino acid uptake. <i>L. lactis</i> N8 contains a total of seven prophage-related fragments and twelve genomic islands. A gene cluster encoding a hybrid NRPS–PKS system that was found in <i>L. lactis</i> N8 reveals that the strain has the potential to synthesize novel secondary metabolites. Furthermore, we have constructed a simplified genome chassis of <i>L. lactis</i> N8 and achieved the largest amount of deletion of <i>L. lactis</i> so far. Taken together, the present study offers further insights into the function and potential role of <i>L. lactis</i> N8 as a model strain of lactic acid bacteria and lays the foundation for its application in the field of synthetic biology. |
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| language | English |
| last_indexed | 2024-03-10T00:53:40Z |
| publishDate | 2021-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-4cfcf89c4b0c4dff95e9a543bd06ddc22023-11-23T14:46:27ZengMDPI AGMicroorganisms2076-26072021-12-011014710.3390/microorganisms10010047Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8Wanjin Qiao0Fulu Liu1Xing Wan2Yu Qiao3Ran Li4Zhenzhou Wu5Per Erik Joakim Saris6Haijin Xu7Mingqiang Qiao8Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, 00014 Helsinki, FinlandKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, 00014 Helsinki, FinlandState Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Protein Sciences, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, 00014 Helsinki, FinlandKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China<i>Lactococcus lactis</i> is a commonly used fermenting bacteria in cheese, beverages and meat products. Due to the lack of simplified chassis strains, it has not been widely used in the fields of synthetic biology. Thus, the construction of lactic acid bacteria chassis strains becomes more and more important. In this study, we performed whole genome sequencing, annotation and analysis of <i>L. lactis</i> N8. Based on the genome analysis, we found that <i>L. lactis</i> N8 contains two large plasmids, and the function prediction of the plasmids shows that some regions are related to carbohydrate transport/metabolism, multi-stress resistance and amino acid uptake. <i>L. lactis</i> N8 contains a total of seven prophage-related fragments and twelve genomic islands. A gene cluster encoding a hybrid NRPS–PKS system that was found in <i>L. lactis</i> N8 reveals that the strain has the potential to synthesize novel secondary metabolites. Furthermore, we have constructed a simplified genome chassis of <i>L. lactis</i> N8 and achieved the largest amount of deletion of <i>L. lactis</i> so far. Taken together, the present study offers further insights into the function and potential role of <i>L. lactis</i> N8 as a model strain of lactic acid bacteria and lays the foundation for its application in the field of synthetic biology.https://www.mdpi.com/2076-2607/10/1/47<i>Lactococcus lactis</i>genomic featureplasmidprophagegenomic islandNRPS–PKS |
| spellingShingle | Wanjin Qiao Fulu Liu Xing Wan Yu Qiao Ran Li Zhenzhou Wu Per Erik Joakim Saris Haijin Xu Mingqiang Qiao Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8 Microorganisms <i>Lactococcus lactis</i> genomic feature plasmid prophage genomic island NRPS–PKS |
| title | Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8 |
| title_full | Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8 |
| title_fullStr | Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8 |
| title_full_unstemmed | Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8 |
| title_short | Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer <i>Lactococcus lactis</i> N8 |
| title_sort | genomic features and construction of streamlined genome chassis of nisin z producer i lactococcus lactis i n8 |
| topic | <i>Lactococcus lactis</i> genomic feature plasmid prophage genomic island NRPS–PKS |
| url | https://www.mdpi.com/2076-2607/10/1/47 |
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