The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis
Many studies have been conducted on the microbial reduction of Pd (II) to palladium nanoparticles (Pd-NPs) due to the environmental friendliness, low cost, and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by <i>Bacillus megaterium<...
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MDPI AG
2024-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/14/6/512 |
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| author | Yuan Chen Jiaxing Wang Daidi Chen Boxi Wang Jinchuan Wu Rongrong Liu Qingxin Li |
| author_facet | Yuan Chen Jiaxing Wang Daidi Chen Boxi Wang Jinchuan Wu Rongrong Liu Qingxin Li |
| author_sort | Yuan Chen |
| collection | DOAJ |
| description | Many studies have been conducted on the microbial reduction of Pd (II) to palladium nanoparticles (Pd-NPs) due to the environmental friendliness, low cost, and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by <i>Bacillus megaterium</i> Y-4 through proteomics. The data are available via ProteomeXchange with identifier PXD049711. Our results revealed that <i>B. megaterium</i> Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh, and fabG and reductase to reduce Pd (II) to Pd-NPs. The expression levels of fabG, tdh, gudB, and rocG that generate NAD(P)H were further increased, and the number of reduced Pd-NPs was further increased with the exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in <i>B. megaterium</i> Y-4 can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by <i>B. megaterium</i> Y-4 at the proteome level. |
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| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-24T17:57:07Z |
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| spelling | doaj.art-4d0263bdf83e44b2aba5f64dee17ca122024-03-27T13:57:33ZengMDPI AGNanomaterials2079-49912024-03-0114651210.3390/nano14060512The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic AnalysisYuan Chen0Jiaxing Wang1Daidi Chen2Boxi Wang3Jinchuan Wu4Rongrong Liu5Qingxin Li6Guangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaMinistry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, School of Environment and Energy, South China University of Technology, Guangzhou 510006, ChinaGuangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaGuangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaKey Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research at Greater Bay Area, Ministry of Education, Guangzhou University, Guangzhou 510006, ChinaGuangdong Provincial Engineering Laboratory of Biomass High Value Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaMany studies have been conducted on the microbial reduction of Pd (II) to palladium nanoparticles (Pd-NPs) due to the environmental friendliness, low cost, and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by <i>Bacillus megaterium</i> Y-4 through proteomics. The data are available via ProteomeXchange with identifier PXD049711. Our results revealed that <i>B. megaterium</i> Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh, and fabG and reductase to reduce Pd (II) to Pd-NPs. The expression levels of fabG, tdh, gudB, and rocG that generate NAD(P)H were further increased, and the number of reduced Pd-NPs was further increased with the exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in <i>B. megaterium</i> Y-4 can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by <i>B. megaterium</i> Y-4 at the proteome level.https://www.mdpi.com/2079-4991/14/6/512palladiumbiological reductionprotein expressionproteomics<i>Bacillus megaterium</i> Y-4 |
| spellingShingle | Yuan Chen Jiaxing Wang Daidi Chen Boxi Wang Jinchuan Wu Rongrong Liu Qingxin Li The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis Nanomaterials palladium biological reduction protein expression proteomics <i>Bacillus megaterium</i> Y-4 |
| title | The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis |
| title_full | The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis |
| title_fullStr | The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis |
| title_full_unstemmed | The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis |
| title_short | The Pd (II) Reduction Mechanisms in <i>Bacillus megaterium</i> Y-4 Revealed by Proteomic Analysis |
| title_sort | pd ii reduction mechanisms in i bacillus megaterium i y 4 revealed by proteomic analysis |
| topic | palladium biological reduction protein expression proteomics <i>Bacillus megaterium</i> Y-4 |
| url | https://www.mdpi.com/2079-4991/14/6/512 |
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