Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis
We have developed a force sensing system to continuously evaluate the mechanical elasticity of micrometer-scale (a few hundred micrometers to a millimeter) live tissues. The sensing is achieved by measuring the deflection of force sensitive cantilevers through microscopic image analysis, which does...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/7/1506 |
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| author | Yuji Tomizawa Krishna Dixit David Daggett Kazunori Hoshino |
| author_facet | Yuji Tomizawa Krishna Dixit David Daggett Kazunori Hoshino |
| author_sort | Yuji Tomizawa |
| collection | DOAJ |
| description | We have developed a force sensing system to continuously evaluate the mechanical elasticity of micrometer-scale (a few hundred micrometers to a millimeter) live tissues. The sensing is achieved by measuring the deflection of force sensitive cantilevers through microscopic image analysis, which does not require electrical strain gauges. Cantilevers made of biocompatible polydimethylsiloxane (PDMS) were actuated by a piezoelectric actuator and functioned as a pair of chopsticks to measure the stiffness of the specimen. The dimensions of the cantilevers were easily adjusted to match the size, range, and stiffness of the zebrafish samples. In this paper, we demonstrated the versatility of this technique by measuring the mechanical elasticity of zebrafish embryos at different stages of development. The stiffness of zebrafish embryos was measured once per hour for 9 h. From the experimental results, we successfully quantified the stiffness change of zebrafish embryos during embryonic development. |
| first_indexed | 2024-04-11T21:57:22Z |
| format | Article |
| id | doaj.art-4d037a5b7bfe4363b559a6d3c7773a54 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T21:57:22Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-4d037a5b7bfe4363b559a6d3c7773a542022-12-22T04:01:03ZengMDPI AGSensors1424-82202019-03-01197150610.3390/s19071506s19071506Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image AnalysisYuji Tomizawa0Krishna Dixit1David Daggett2Kazunori Hoshino3Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USADepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USADepartment of Molecular & Cell Biology, University of Connecticut, Storrs, CT 06269, USADepartment of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USAWe have developed a force sensing system to continuously evaluate the mechanical elasticity of micrometer-scale (a few hundred micrometers to a millimeter) live tissues. The sensing is achieved by measuring the deflection of force sensitive cantilevers through microscopic image analysis, which does not require electrical strain gauges. Cantilevers made of biocompatible polydimethylsiloxane (PDMS) were actuated by a piezoelectric actuator and functioned as a pair of chopsticks to measure the stiffness of the specimen. The dimensions of the cantilevers were easily adjusted to match the size, range, and stiffness of the zebrafish samples. In this paper, we demonstrated the versatility of this technique by measuring the mechanical elasticity of zebrafish embryos at different stages of development. The stiffness of zebrafish embryos was measured once per hour for 9 h. From the experimental results, we successfully quantified the stiffness change of zebrafish embryos during embryonic development.https://www.mdpi.com/1424-8220/19/7/1506stiffness analysisforce sensorzebrafish embryobiosolid mechanicssoft lithography |
| spellingShingle | Yuji Tomizawa Krishna Dixit David Daggett Kazunori Hoshino Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis Sensors stiffness analysis force sensor zebrafish embryo biosolid mechanics soft lithography |
| title | Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis |
| title_full | Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis |
| title_fullStr | Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis |
| title_full_unstemmed | Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis |
| title_short | Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis |
| title_sort | biocompatible cantilevers for mechanical characterization of zebrafish embryos using image analysis |
| topic | stiffness analysis force sensor zebrafish embryo biosolid mechanics soft lithography |
| url | https://www.mdpi.com/1424-8220/19/7/1506 |
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