Self-cleaving guide RNAs enable pharmacological selection of precise gene editing events in vivo
Here, authors show that precise integration of an rAAV vector into a chromosomal target locus results in expression of a self-cleaving guide RNA. This recombination event creates genetic resistance to a hepatotoxic compound and enables in vivo expansion of gene-edited hepatocytes using a selection d...
Main Authors: | Amita Tiyaboonchai, Anne Vonada, Jeffrey Posey, Carl Pelz, Leslie Wakefield, Markus Grompe |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2022-11-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-022-35097-5 |
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