Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin
Background: P120(ctn) is a specific membranous adhesion protein, that maintains the stability of intercellular junctions. An altered expression of p120(ctn), either reduced in the cell membrane or increase in the cytoplasm, plays a crucial role in carcinogenesis. No research has analysed the express...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Karolinum Press
2017-06-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/60/1/0032/ |
| _version_ | 1819155293110534144 |
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| author | Vladimír Bartoš Milada Kullová |
| author_facet | Vladimír Bartoš Milada Kullová |
| author_sort | Vladimír Bartoš |
| collection | DOAJ |
| description | Background: P120(ctn) is a specific membranous adhesion protein, that maintains the stability of intercellular junctions. An altered expression of p120(ctn), either reduced in the cell membrane or increase in the cytoplasm, plays a crucial role in carcinogenesis. No research has analysed the expression of p120(ctn) in basal cell carcinoma (BCC) of the skin so far. Therefore, we immunohistochemically studied p120(ctn) in a set of cutaneous BCCs in order to determine, whether there is difference in the expression pattern related to the histologic subtypes and tumor growth characteristics. Material and Methods: The study group consisted of 38 BCCs cathegorized into low-risk (non-infiltrative) subroup (8 superficial and 12 nodular subtypes) and high-risk (infiltrative) subgroup (10 nodular-infiltrative and 8 infiltrative subtypes). Specific monoclonal antibody against p120(ctn) was used for staining. Results: Overall, there were 12 cases (31.6%) with normal preserved and 26 cases (68.4%) with abnormal p120(ctn) expression. In superficial, nodular, nodular-infiltrative and infiltrative subtypes, abnormal p120(ctn) immunoreactivity was found in 37.5% (3/8), 41.7% (5/12), 100% (10/10) and 100% (8/8), respectively. We have confirmed a strong correlation between the expression of p120(ctn) and both given, non-infiltrative and infiltrative BCC growth phenotypes. In the latter subgroup, almost all lesions showed diffusely reduced membranous staining, of which five also manifested an aberrant immunoreactivity in the cytoplasm. This cytoplasmic positivity occurred solely at the invasive front of the infiltrative tumor formations. Conclusion: Our results showed that decreased membranous expression of p120(ctn) was a frequent event in human cutaneous BCC and it was associated with infiltrative growth phenotype. Considering that nearly half of the BCCs with non-infiltrative growth pattern also exhibited reduced membranous expression, aberrant cytoplasmic immunoreactivity of p120(ctn), which was found exclusively in the high-risk BCC variants, can more reliably reflect and predict biological behaviour and malignant potential. |
| first_indexed | 2024-12-22T15:34:40Z |
| format | Article |
| id | doaj.art-4d099ba9d3044efeb8191325bf5db0b5 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-22T15:34:40Z |
| publishDate | 2017-06-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-4d099ba9d3044efeb8191325bf5db0b52022-12-21T18:21:17ZengKarolinum PressActa Medica1211-42861805-96942017-06-01601323610.14712/18059694.2017.485145Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the SkinVladimír BartošMilada KullováBackground: P120(ctn) is a specific membranous adhesion protein, that maintains the stability of intercellular junctions. An altered expression of p120(ctn), either reduced in the cell membrane or increase in the cytoplasm, plays a crucial role in carcinogenesis. No research has analysed the expression of p120(ctn) in basal cell carcinoma (BCC) of the skin so far. Therefore, we immunohistochemically studied p120(ctn) in a set of cutaneous BCCs in order to determine, whether there is difference in the expression pattern related to the histologic subtypes and tumor growth characteristics. Material and Methods: The study group consisted of 38 BCCs cathegorized into low-risk (non-infiltrative) subroup (8 superficial and 12 nodular subtypes) and high-risk (infiltrative) subgroup (10 nodular-infiltrative and 8 infiltrative subtypes). Specific monoclonal antibody against p120(ctn) was used for staining. Results: Overall, there were 12 cases (31.6%) with normal preserved and 26 cases (68.4%) with abnormal p120(ctn) expression. In superficial, nodular, nodular-infiltrative and infiltrative subtypes, abnormal p120(ctn) immunoreactivity was found in 37.5% (3/8), 41.7% (5/12), 100% (10/10) and 100% (8/8), respectively. We have confirmed a strong correlation between the expression of p120(ctn) and both given, non-infiltrative and infiltrative BCC growth phenotypes. In the latter subgroup, almost all lesions showed diffusely reduced membranous staining, of which five also manifested an aberrant immunoreactivity in the cytoplasm. This cytoplasmic positivity occurred solely at the invasive front of the infiltrative tumor formations. Conclusion: Our results showed that decreased membranous expression of p120(ctn) was a frequent event in human cutaneous BCC and it was associated with infiltrative growth phenotype. Considering that nearly half of the BCCs with non-infiltrative growth pattern also exhibited reduced membranous expression, aberrant cytoplasmic immunoreactivity of p120(ctn), which was found exclusively in the high-risk BCC variants, can more reliably reflect and predict biological behaviour and malignant potential.https://actamedica.lfhk.cuni.cz/60/1/0032/basal cell carcinomabiological behaviourP120catenin |
| spellingShingle | Vladimír Bartoš Milada Kullová Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin Acta Medica basal cell carcinoma biological behaviour P120catenin |
| title | Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin |
| title_full | Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin |
| title_fullStr | Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin |
| title_full_unstemmed | Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin |
| title_short | Investigation of P120catenin Expression in Human Basal Cell Carcinoma of the Skin |
| title_sort | investigation of p120catenin expression in human basal cell carcinoma of the skin |
| topic | basal cell carcinoma biological behaviour P120catenin |
| url | https://actamedica.lfhk.cuni.cz/60/1/0032/ |
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