Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat
The present study aimed to reveal the impact of the stay-green trait in bread wheat under terminal heat stress. Field experiments (early and late sowing; for two consecutive years) were conducted to investigate the influence of terminal heat stress on the morpho-physiological traits in different sta...
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MDPI AG
2020-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/7/1001 |
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| author | Sadia Latif Liping Wang Jahangir Khan Zeshan Ali Sunish Kumar Sehgal Md Ali Babar Jianping Wang Umar Masood Quraishi |
| author_facet | Sadia Latif Liping Wang Jahangir Khan Zeshan Ali Sunish Kumar Sehgal Md Ali Babar Jianping Wang Umar Masood Quraishi |
| author_sort | Sadia Latif |
| collection | DOAJ |
| description | The present study aimed to reveal the impact of the stay-green trait in bread wheat under terminal heat stress. Field experiments (early and late sowing; for two consecutive years) were conducted to investigate the influence of terminal heat stress on the morpho-physiological traits in different stay-green types i.e., non-stay-green, moderately non-stay-green, moderately stay-green, and stay-green. In addition, the greenhouse experiment was performed to dissect the stay-green trait in functional stay-green, non-functional stay-green, and non-stay-green genotypes. The results of the field experiments confirmed that genotypes exhibiting the stay-green trait have a significantly high chlorophyll content, normalized difference vegetative index, grain yield, biological yield, kernel weight, and low canopy temperature under control and heat stress conditions. In the greenhouse experiment, functional stay-green and non-functional stay-green genotypes showed a high chlorophyll content and photochemical efficiency, whereas biological yield and grain yield showed a significant relation with the functional stay-green genotype under control and terminal heat stress treatments. The sequencing and expression analysis of chlorophyllide a oxygenase (CaO), light-harvesting complex (Cab), stay-green (SGR), and red chlorophyll catabolite reductase (RCCR) in functional stay-green, non-functional stay-green, and non-stay-green genotypes revealed variations in the exons of CaO and RCCR; and significant difference in the regulation of CaO and Cab at 7 days after anthesis under terminal heat stress. This study confirms that genotypes displaying the stay-green trait can aid wheat breeders to cope with increasing temperature in the impending decades. |
| first_indexed | 2024-03-10T18:31:26Z |
| format | Article |
| id | doaj.art-4d09cbc43b044a3fbd1e0c7fe33a1e7a |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T18:31:26Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-4d09cbc43b044a3fbd1e0c7fe33a1e7a2023-11-20T06:32:24ZengMDPI AGAgronomy2073-43952020-07-01107100110.3390/agronomy10071001Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread WheatSadia Latif0Liping Wang1Jahangir Khan2Zeshan Ali3Sunish Kumar Sehgal4Md Ali Babar5Jianping Wang6Umar Masood Quraishi7Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Agronomy, University of Florida, Gainesville, FL 32611, USADepartment of Agronomy, University of Florida, Gainesville, FL 32611, USAPlant Physiology Program, Crop Sciences Institute, National Agricultural Research Centre, Islamabad 45500, PakistanDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007-1096, USADepartment of Agronomy, University of Florida, Gainesville, FL 32611, USADepartment of Agronomy, University of Florida, Gainesville, FL 32611, USADepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanThe present study aimed to reveal the impact of the stay-green trait in bread wheat under terminal heat stress. Field experiments (early and late sowing; for two consecutive years) were conducted to investigate the influence of terminal heat stress on the morpho-physiological traits in different stay-green types i.e., non-stay-green, moderately non-stay-green, moderately stay-green, and stay-green. In addition, the greenhouse experiment was performed to dissect the stay-green trait in functional stay-green, non-functional stay-green, and non-stay-green genotypes. The results of the field experiments confirmed that genotypes exhibiting the stay-green trait have a significantly high chlorophyll content, normalized difference vegetative index, grain yield, biological yield, kernel weight, and low canopy temperature under control and heat stress conditions. In the greenhouse experiment, functional stay-green and non-functional stay-green genotypes showed a high chlorophyll content and photochemical efficiency, whereas biological yield and grain yield showed a significant relation with the functional stay-green genotype under control and terminal heat stress treatments. The sequencing and expression analysis of chlorophyllide a oxygenase (CaO), light-harvesting complex (Cab), stay-green (SGR), and red chlorophyll catabolite reductase (RCCR) in functional stay-green, non-functional stay-green, and non-stay-green genotypes revealed variations in the exons of CaO and RCCR; and significant difference in the regulation of CaO and Cab at 7 days after anthesis under terminal heat stress. This study confirms that genotypes displaying the stay-green trait can aid wheat breeders to cope with increasing temperature in the impending decades.https://www.mdpi.com/2073-4395/10/7/1001<i>Triticum aestivum</i> L.terminal heat stressstay greenchlorophyllide a oxygenase genelight-harvesting complex genestay-green gene |
| spellingShingle | Sadia Latif Liping Wang Jahangir Khan Zeshan Ali Sunish Kumar Sehgal Md Ali Babar Jianping Wang Umar Masood Quraishi Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat Agronomy <i>Triticum aestivum</i> L. terminal heat stress stay green chlorophyllide a oxygenase gene light-harvesting complex gene stay-green gene |
| title | Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat |
| title_full | Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat |
| title_fullStr | Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat |
| title_full_unstemmed | Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat |
| title_short | Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat |
| title_sort | deciphering the role of stay green trait to mitigate terminal heat stress in bread wheat |
| topic | <i>Triticum aestivum</i> L. terminal heat stress stay green chlorophyllide a oxygenase gene light-harvesting complex gene stay-green gene |
| url | https://www.mdpi.com/2073-4395/10/7/1001 |
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