Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates
Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable tar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/5/2/568 |
| _version_ | 1818217365809135616 |
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| author | Se Chang Park Sang Phil Shin Casiano Choresca Jr. Ji Hyung Kim Tristan Renault Jee Eun Han Jin Woo Jun |
| author_facet | Se Chang Park Sang Phil Shin Casiano Choresca Jr. Ji Hyung Kim Tristan Renault Jee Eun Han Jin Woo Jun |
| author_sort | Se Chang Park |
| collection | DOAJ |
| description | Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies. |
| first_indexed | 2024-12-12T07:06:43Z |
| format | Article |
| id | doaj.art-4d09e0f314a74fa19ac564d2cdd2db35 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-12T07:06:43Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4d09e0f314a74fa19ac564d2cdd2db352022-12-22T00:33:43ZengMDPI AGViruses1999-49152013-01-015256857610.3390/v5020568Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 IsolatesSe Chang ParkSang Phil ShinCasiano Choresca Jr.Ji Hyung KimTristan RenaultJee Eun HanJin Woo JunCyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies.http://www.mdpi.com/1999-4915/5/2/568Cyprinid herpes virus 3glycoprotein geneKoi herpesvirusmicrosatellitepolymorphismphylogenetic tree |
| spellingShingle | Se Chang Park Sang Phil Shin Casiano Choresca Jr. Ji Hyung Kim Tristan Renault Jee Eun Han Jin Woo Jun Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates Viruses Cyprinid herpes virus 3 glycoprotein gene Koi herpesvirus microsatellite polymorphism phylogenetic tree |
| title | Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates |
| title_full | Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates |
| title_fullStr | Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates |
| title_full_unstemmed | Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates |
| title_short | Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates |
| title_sort | identifying the viral genes encoding envelope glycoproteins for differentiation of cyprinid herpesvirus 3 isolates |
| topic | Cyprinid herpes virus 3 glycoprotein gene Koi herpesvirus microsatellite polymorphism phylogenetic tree |
| url | http://www.mdpi.com/1999-4915/5/2/568 |
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