Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut
The benefits of probiotics for constipation are widely accepted, but the mechanisms involving gut metabolites are unclear. In this study, we investigated the effects of Lactobacillus casei strain Shirota (LcS) on constipated patients and revealed that a metabolite mediator is involved in the LcS-ind...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00324/full |
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| author | Yangwenshan Ou Yangwenshan Ou Shanbin Chen Shanbin Chen Fazheng Ren Fazheng Ren Fazheng Ren Ming Zhang Shaoyang Ge Shaoyang Ge Huiyuan Guo Huiyuan Guo Hao Zhang Hao Zhang Hao Zhang Liang Zhao Liang Zhao Liang Zhao |
| author_facet | Yangwenshan Ou Yangwenshan Ou Shanbin Chen Shanbin Chen Fazheng Ren Fazheng Ren Fazheng Ren Ming Zhang Shaoyang Ge Shaoyang Ge Huiyuan Guo Huiyuan Guo Hao Zhang Hao Zhang Hao Zhang Liang Zhao Liang Zhao Liang Zhao |
| author_sort | Yangwenshan Ou |
| collection | DOAJ |
| description | The benefits of probiotics for constipation are widely accepted, but the mechanisms involving gut metabolites are unclear. In this study, we investigated the effects of Lactobacillus casei strain Shirota (LcS) on constipated patients and revealed that a metabolite mediator is involved in the LcS-induced constipation alleviation. Sixteen constipated patients and 22 non-constipated participants were recruited. The subjects consumed 100 mL of an LcS beverage (108 CFU/mL) per day for 28 days. The fecal non-volatile metabolites were determined by GC/MS, and the targeted metabolites were further verified in a constipated mouse model. In constipated patients, LcS intervention significantly improved defecation frequency (from 4.81 to 7.81 times per week, p < 0.05), stool consistency (from 2.52 to 3.68, p < 0.05) and constipation-related symptoms. A total of 14 non-volatile fecal metabolites were obtained as potential constipation-related metabolites that were regulated by LcS. Among these metabolites, pipecolinic acid (PIPA) had a significant positive correlation with defecation frequency in constipated patients. PIPA significantly promoted the small intestinal propulsive rate (from 25.45 to 39.68%) and increased the number of fecal pellets (from 30.38 to 57.38 pellets) in constipated mice (p < 0.05). The 5-hydroxytryptamine (5-HT) and acetylcholine (ACh) in colonic tissue may be partly involved in PIPA-mediated constipation alleviation. In conclusion, PIPA was a metabolic mediator in the gut that participated in LcS-induced constipation alleviation. |
| first_indexed | 2024-12-24T03:44:42Z |
| format | Article |
| id | doaj.art-4d0b717a353b40ffb8158654e576084e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T03:44:42Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-4d0b717a353b40ffb8158654e576084e2022-12-21T17:16:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00324443785Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the GutYangwenshan Ou0Yangwenshan Ou1Shanbin Chen2Shanbin Chen3Fazheng Ren4Fazheng Ren5Fazheng Ren6Ming Zhang7Shaoyang Ge8Shaoyang Ge9Huiyuan Guo10Huiyuan Guo11Hao Zhang12Hao Zhang13Hao Zhang14Liang Zhao15Liang Zhao16Liang Zhao17Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaSchool of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, ChinaBeijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaHebei Engineering Research Center of Animal Product, Sanhe, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaThe benefits of probiotics for constipation are widely accepted, but the mechanisms involving gut metabolites are unclear. In this study, we investigated the effects of Lactobacillus casei strain Shirota (LcS) on constipated patients and revealed that a metabolite mediator is involved in the LcS-induced constipation alleviation. Sixteen constipated patients and 22 non-constipated participants were recruited. The subjects consumed 100 mL of an LcS beverage (108 CFU/mL) per day for 28 days. The fecal non-volatile metabolites were determined by GC/MS, and the targeted metabolites were further verified in a constipated mouse model. In constipated patients, LcS intervention significantly improved defecation frequency (from 4.81 to 7.81 times per week, p < 0.05), stool consistency (from 2.52 to 3.68, p < 0.05) and constipation-related symptoms. A total of 14 non-volatile fecal metabolites were obtained as potential constipation-related metabolites that were regulated by LcS. Among these metabolites, pipecolinic acid (PIPA) had a significant positive correlation with defecation frequency in constipated patients. PIPA significantly promoted the small intestinal propulsive rate (from 25.45 to 39.68%) and increased the number of fecal pellets (from 30.38 to 57.38 pellets) in constipated mice (p < 0.05). The 5-hydroxytryptamine (5-HT) and acetylcholine (ACh) in colonic tissue may be partly involved in PIPA-mediated constipation alleviation. In conclusion, PIPA was a metabolic mediator in the gut that participated in LcS-induced constipation alleviation.https://www.frontiersin.org/article/10.3389/fmicb.2019.00324/fullLactobacillus casei strain Shirotaconstipationmetabolomicsgut metabolitespipecolinic acid |
| spellingShingle | Yangwenshan Ou Yangwenshan Ou Shanbin Chen Shanbin Chen Fazheng Ren Fazheng Ren Fazheng Ren Ming Zhang Shaoyang Ge Shaoyang Ge Huiyuan Guo Huiyuan Guo Hao Zhang Hao Zhang Hao Zhang Liang Zhao Liang Zhao Liang Zhao Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut Frontiers in Microbiology Lactobacillus casei strain Shirota constipation metabolomics gut metabolites pipecolinic acid |
| title | Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut |
| title_full | Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut |
| title_fullStr | Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut |
| title_full_unstemmed | Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut |
| title_short | Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut |
| title_sort | lactobacillus casei strain shirota alleviates constipation in adults by increasing the pipecolinic acid level in the gut |
| topic | Lactobacillus casei strain Shirota constipation metabolomics gut metabolites pipecolinic acid |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00324/full |
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