High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle
Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, c...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831338/?tool=EBI |
| _version_ | 1797952772176347136 |
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| author | Natália Marins Bastos Rodrigo Silva Goulart Danilo Brito Bambil Alessandra Bridi Rosane Mazzarella Luana Alves Paola Maria da Silva Rosa Adomar Laurindo Neto Saulo Luz Silva Miguel Henrique de Almeida Santana João Alberto Negrão Guilherme Pugliesi Flávio Vieira Meirelles Felipe Perecin Juliano Coelho da Silveira |
| author_facet | Natália Marins Bastos Rodrigo Silva Goulart Danilo Brito Bambil Alessandra Bridi Rosane Mazzarella Luana Alves Paola Maria da Silva Rosa Adomar Laurindo Neto Saulo Luz Silva Miguel Henrique de Almeida Santana João Alberto Negrão Guilherme Pugliesi Flávio Vieira Meirelles Felipe Perecin Juliano Coelho da Silveira |
| author_sort | Natália Marins Bastos |
| collection | DOAJ |
| description | Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, cows were weekly weighted and evaluated for subcutaneous fat thickness and insulin serum concentration along the experimental period. At the end of the experimental period, animals were submitted to estrous synchronization and artificial insemination. Animals were slaughtered approximately 120 h after ovulation induction and the reproductive tracts were collected for embryo recovery and samples collection. Cumulus-oocyte-complexes (COC) and follicular fluid were collected from 3–6 mm in diameter ovarian follicles to perform miRNA analysis of cumulus cells (CC) and extracellular vesicles from follicular fluid (EV FF). As expected, differences were observed among MBER and HBER groups for body weight, fat thickness, and insulin serum concentration. HBER animals showed lower ovulation and embryo recovery rates compared to MBER animals. Different miRNAs were found among CC and EV FF within groups, suggesting that the BER may influence follicular communication. This suggests that small follicles (3–6 mm diameter) are already under BER effects, which may be greater on later stages of follicular development. |
| first_indexed | 2024-04-10T22:51:29Z |
| format | Article |
| id | doaj.art-4d0f7820702b48ecaa8782f5a82dd076 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T22:51:29Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4d0f7820702b48ecaa8782f5a82dd0762023-01-15T05:31:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicleNatália Marins BastosRodrigo Silva GoulartDanilo Brito BambilAlessandra BridiRosane MazzarellaLuana AlvesPaola Maria da Silva RosaAdomar Laurindo NetoSaulo Luz SilvaMiguel Henrique de Almeida SantanaJoão Alberto NegrãoGuilherme PugliesiFlávio Vieira MeirellesFelipe PerecinJuliano Coelho da SilveiraAiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, cows were weekly weighted and evaluated for subcutaneous fat thickness and insulin serum concentration along the experimental period. At the end of the experimental period, animals were submitted to estrous synchronization and artificial insemination. Animals were slaughtered approximately 120 h after ovulation induction and the reproductive tracts were collected for embryo recovery and samples collection. Cumulus-oocyte-complexes (COC) and follicular fluid were collected from 3–6 mm in diameter ovarian follicles to perform miRNA analysis of cumulus cells (CC) and extracellular vesicles from follicular fluid (EV FF). As expected, differences were observed among MBER and HBER groups for body weight, fat thickness, and insulin serum concentration. HBER animals showed lower ovulation and embryo recovery rates compared to MBER animals. Different miRNAs were found among CC and EV FF within groups, suggesting that the BER may influence follicular communication. This suggests that small follicles (3–6 mm diameter) are already under BER effects, which may be greater on later stages of follicular development.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831338/?tool=EBI |
| spellingShingle | Natália Marins Bastos Rodrigo Silva Goulart Danilo Brito Bambil Alessandra Bridi Rosane Mazzarella Luana Alves Paola Maria da Silva Rosa Adomar Laurindo Neto Saulo Luz Silva Miguel Henrique de Almeida Santana João Alberto Negrão Guilherme Pugliesi Flávio Vieira Meirelles Felipe Perecin Juliano Coelho da Silveira High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle PLoS ONE |
| title | High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle |
| title_full | High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle |
| title_fullStr | High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle |
| title_full_unstemmed | High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle |
| title_short | High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle |
| title_sort | high body energy reserve influences extracellular vesicles mirna contents within the ovarian follicle |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9831338/?tool=EBI |
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