The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study
OBJECTIVES: We evaluated mRNA expression levels of genes that encode TGF-β1; the TGF-β1 receptor; the collagen-modifying enzymes LOX, PLOD1, and PLOD2; and the extracellular matrix proteins COMP, FN1, TNC and TNXB in synovial/capsule specimens from patients with idiopathic adhesive capsulitis. Possi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
|
| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322016000600325&lng=en&tlng=en |
| _version_ | 1811329951146704896 |
|---|---|
| author | Carina Cohen Mariana Ferreira Leal Paulo Santoro Belangero Eduardo Antônio Figueiredo Marília Cardoso Smith Carlos Vicente Andreoli Alberto de Castro Pochini Moises Cohen Benno Ejnisman Flávio Faloppa |
| author_facet | Carina Cohen Mariana Ferreira Leal Paulo Santoro Belangero Eduardo Antônio Figueiredo Marília Cardoso Smith Carlos Vicente Andreoli Alberto de Castro Pochini Moises Cohen Benno Ejnisman Flávio Faloppa |
| author_sort | Carina Cohen |
| collection | DOAJ |
| description | OBJECTIVES: We evaluated mRNA expression levels of genes that encode TGF-β1; the TGF-β1 receptor; the collagen-modifying enzymes LOX, PLOD1, and PLOD2; and the extracellular matrix proteins COMP, FN1, TNC and TNXB in synovial/capsule specimens from patients with idiopathic adhesive capsulitis. Possible associations between the measured mRNA levels and clinical parameters were also investigated. METHODS: We obtained glenohumeral joint synovium/capsule specimens from 9 patients with idiopathic adhesive capsulitis who had not shown improvement in symptoms after 5 months of physiotherapy. Adhesive capsulitis was confirmed in all patients by magnetic resonance imaging. We also obtained specimens from 8 control patients who had underwent surgery for acute acromioclavicular joint dislocation and who had radiological indication of glenohumeral capsule alteration based on arthroscopic evaluation. mRNA expression in the synovium/capsule specimens was analyzed by quantitative reverse transcription PCR. The B2M and HPRT1 genes were used as references to normalize target gene expression in the shoulder tissue samples. RESULTS: The synovium/capsule samples from the patients with adhesive capsulitis had significantly higher TNC and FN1 expression than those from the controls. Additionally, symptom duration directly correlated with expression of TGFβ1 receptor I. CONCLUSION: Elevated levels of TNC and FN1 expression may be a marker of capsule injury. Upregulation of TGFβ1 receptor I seems to be dependent on symptom duration; therefore, TGFβ signaling may be involved in adhesive capsulitis. As such, TNC, FN1 and TGFβ1 receptor I may also play roles in adhesive capsulitis by contributing to capsule inflammation and fibrosis. |
| first_indexed | 2024-04-13T15:53:03Z |
| format | Article |
| id | doaj.art-4d140db5fd124a1395a8a918ab375d41 |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-04-13T15:53:03Z |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-4d140db5fd124a1395a8a918ab375d412022-12-22T02:40:47ZengElsevier EspañaClinics1807-59321980-532271632533110.6061/clinics/2016(06)07S1807-59322016000600325The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression studyCarina CohenMariana Ferreira LealPaulo Santoro BelangeroEduardo Antônio FigueiredoMarília Cardoso SmithCarlos Vicente AndreoliAlberto de Castro PochiniMoises CohenBenno EjnismanFlávio FaloppaOBJECTIVES: We evaluated mRNA expression levels of genes that encode TGF-β1; the TGF-β1 receptor; the collagen-modifying enzymes LOX, PLOD1, and PLOD2; and the extracellular matrix proteins COMP, FN1, TNC and TNXB in synovial/capsule specimens from patients with idiopathic adhesive capsulitis. Possible associations between the measured mRNA levels and clinical parameters were also investigated. METHODS: We obtained glenohumeral joint synovium/capsule specimens from 9 patients with idiopathic adhesive capsulitis who had not shown improvement in symptoms after 5 months of physiotherapy. Adhesive capsulitis was confirmed in all patients by magnetic resonance imaging. We also obtained specimens from 8 control patients who had underwent surgery for acute acromioclavicular joint dislocation and who had radiological indication of glenohumeral capsule alteration based on arthroscopic evaluation. mRNA expression in the synovium/capsule specimens was analyzed by quantitative reverse transcription PCR. The B2M and HPRT1 genes were used as references to normalize target gene expression in the shoulder tissue samples. RESULTS: The synovium/capsule samples from the patients with adhesive capsulitis had significantly higher TNC and FN1 expression than those from the controls. Additionally, symptom duration directly correlated with expression of TGFβ1 receptor I. CONCLUSION: Elevated levels of TNC and FN1 expression may be a marker of capsule injury. Upregulation of TGFβ1 receptor I seems to be dependent on symptom duration; therefore, TGFβ signaling may be involved in adhesive capsulitis. As such, TNC, FN1 and TGFβ1 receptor I may also play roles in adhesive capsulitis by contributing to capsule inflammation and fibrosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322016000600325&lng=en&tlng=enadhesive capsulitisglenohumeral capsulegene expressionextracellular matrixtgfβ1 signaling |
| spellingShingle | Carina Cohen Mariana Ferreira Leal Paulo Santoro Belangero Eduardo Antônio Figueiredo Marília Cardoso Smith Carlos Vicente Andreoli Alberto de Castro Pochini Moises Cohen Benno Ejnisman Flávio Faloppa The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study Clinics adhesive capsulitis glenohumeral capsule gene expression extracellular matrix tgfβ1 signaling |
| title | The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study |
| title_full | The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study |
| title_fullStr | The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study |
| title_full_unstemmed | The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study |
| title_short | The roles of Tenascin C and Fibronectin 1 in adhesive capsulitis: a pilot gene expression study |
| title_sort | roles of tenascin c and fibronectin 1 in adhesive capsulitis a pilot gene expression study |
| topic | adhesive capsulitis glenohumeral capsule gene expression extracellular matrix tgfβ1 signaling |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322016000600325&lng=en&tlng=en |
| work_keys_str_mv | AT carinacohen therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT marianaferreiraleal therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT paulosantorobelangero therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT eduardoantoniofigueiredo therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT mariliacardososmith therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT carlosvicenteandreoli therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT albertodecastropochini therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT moisescohen therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT bennoejnisman therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT flaviofaloppa therolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT carinacohen rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT marianaferreiraleal rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT paulosantorobelangero rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT eduardoantoniofigueiredo rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT mariliacardososmith rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT carlosvicenteandreoli rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT albertodecastropochini rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT moisescohen rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT bennoejnisman rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy AT flaviofaloppa rolesoftenascincandfibronectin1inadhesivecapsulitisapilotgeneexpressionstudy |