Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens
Macrophages, as professional phagocytes of the immune system, possess the ability to detect and clear invading pathogens and apoptotic cells through phagocytosis. Phagocytosis involves membrane reorganization and remodeling events on the cell surface, which play an essential role in innate immunity...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.01723/full |
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| author | Julio M. Rubio Julio M. Rubio Alma M. Astudillo Alma M. Astudillo Javier Casas Javier Casas María A. Balboa María A. Balboa Jesús Balsinde Jesús Balsinde |
| author_facet | Julio M. Rubio Julio M. Rubio Alma M. Astudillo Alma M. Astudillo Javier Casas Javier Casas María A. Balboa María A. Balboa Jesús Balsinde Jesús Balsinde |
| author_sort | Julio M. Rubio |
| collection | DOAJ |
| description | Macrophages, as professional phagocytes of the immune system, possess the ability to detect and clear invading pathogens and apoptotic cells through phagocytosis. Phagocytosis involves membrane reorganization and remodeling events on the cell surface, which play an essential role in innate immunity and tissue homeostasis and the control of inflammation. In this work, we report that cells deficient in membrane ethanolamine plasmalogen demonstrate a reduced capacity to phagocytize opsonized zymosan particles. Amelioration of plasmalogen deficiency in these cells by incubation with lysoplasmalogen results in a significant augmentation of the phagocytic capacity of the cells. In parallel with these increases, restoration of plasmalogen levels in the cells also increases the number and size of lipid rafts in the membrane, reduces membrane fluidity down to levels found in cells containing normal plasmalogen levels, and improves receptor-mediated signaling. Collectively, these results suggest that membrane plasmalogen level determines characteristics of the plasma membrane such as fluidity and the formation of microdomains that are necessary for efficient signal transduction leading to optimal phagocytosis by macrophages. |
| first_indexed | 2024-12-19T10:16:48Z |
| format | Article |
| id | doaj.art-4d17c8110cf34428a498f595c810480b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T10:16:48Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4d17c8110cf34428a498f595c810480b2022-12-21T20:26:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-07-01910.3389/fimmu.2018.01723377598Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine PlasmalogensJulio M. Rubio0Julio M. Rubio1Alma M. Astudillo2Alma M. Astudillo3Javier Casas4Javier Casas5María A. Balboa6María A. Balboa7Jesús Balsinde8Jesús Balsinde9Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Valladolid, Valladolid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Madrid, SpainInstituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Valladolid, Valladolid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Madrid, SpainInstituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Valladolid, Valladolid, SpainDepartamento de Bioquímica y Fisiología, Universidad de Valladolid, Valladolid, SpainInstituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Valladolid, Valladolid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Madrid, SpainInstituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Valladolid, Valladolid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Madrid, SpainMacrophages, as professional phagocytes of the immune system, possess the ability to detect and clear invading pathogens and apoptotic cells through phagocytosis. Phagocytosis involves membrane reorganization and remodeling events on the cell surface, which play an essential role in innate immunity and tissue homeostasis and the control of inflammation. In this work, we report that cells deficient in membrane ethanolamine plasmalogen demonstrate a reduced capacity to phagocytize opsonized zymosan particles. Amelioration of plasmalogen deficiency in these cells by incubation with lysoplasmalogen results in a significant augmentation of the phagocytic capacity of the cells. In parallel with these increases, restoration of plasmalogen levels in the cells also increases the number and size of lipid rafts in the membrane, reduces membrane fluidity down to levels found in cells containing normal plasmalogen levels, and improves receptor-mediated signaling. Collectively, these results suggest that membrane plasmalogen level determines characteristics of the plasma membrane such as fluidity and the formation of microdomains that are necessary for efficient signal transduction leading to optimal phagocytosis by macrophages.https://www.frontiersin.org/article/10.3389/fimmu.2018.01723/fullethanolamine plasmalogenphagocytosisarachidonic acidinflammationmonocytes/macrophages |
| spellingShingle | Julio M. Rubio Julio M. Rubio Alma M. Astudillo Alma M. Astudillo Javier Casas Javier Casas María A. Balboa María A. Balboa Jesús Balsinde Jesús Balsinde Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens Frontiers in Immunology ethanolamine plasmalogen phagocytosis arachidonic acid inflammation monocytes/macrophages |
| title | Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens |
| title_full | Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens |
| title_fullStr | Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens |
| title_full_unstemmed | Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens |
| title_short | Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens |
| title_sort | regulation of phagocytosis in macrophages by membrane ethanolamine plasmalogens |
| topic | ethanolamine plasmalogen phagocytosis arachidonic acid inflammation monocytes/macrophages |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.01723/full |
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