Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation
With the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to mai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/666 |
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| author | Lyanne Rodríguez Lina Badimon Diego Méndez Teresa Padró Gemma Vilahur Esther Peña Basilio Carrasco Hermine Vogel Iván Palomo Eduardo Fuentes |
| author_facet | Lyanne Rodríguez Lina Badimon Diego Méndez Teresa Padró Gemma Vilahur Esther Peña Basilio Carrasco Hermine Vogel Iván Palomo Eduardo Fuentes |
| author_sort | Lyanne Rodríguez |
| collection | DOAJ |
| description | With the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to maintaining good cardiovascular health. In this work, we evaluate the flavonol isorhamnetin’s antiplatelet effect on human platelets, using collagen, thrombin receptor activator peptide 6 (TRAP-6), and phorbol myristate acetate (PMA) as agonists. Isorhamnetin induced a significant inhibition on collagen- and TRAP-6-induced platelet aggregation, with half-maximum inhibitory concentration (IC<sub>50</sub>) values of 8.1 ± 2.6 and 16.1 ± 11.1 µM, respectively; while it did not show cytotoxic effect. Isorhamnetin reduced adenosine triphosphate levels (ATP) in platelets stimulated by collagen and TRAP-6. We also evidenced that isorhamnetin’s antiplatelet activity was related to the inhibition of mitochondrial function without effect on reactive oxygen species (ROS) levels. Additionally, we investigated isorhamnetin’s effect on thrombus formation in vitro under flow conditions on the damaged vessel wall. In this context, we demonstrate that isorhamnetin at 20 µM induced a significant inhibition on platelet deposition, confirming its antithrombotic effect. Our findings corroborate the antiplatelet and antithrombotic potential of isorhamnetin present in many foods of daily consumption. |
| first_indexed | 2024-03-10T11:59:46Z |
| format | Article |
| id | doaj.art-4d1c78024b604cd2bd754e5c9f0bc4e7 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:59:46Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4d1c78024b604cd2bd754e5c9f0bc4e72023-11-21T17:02:44ZengMDPI AGAntioxidants2076-39212021-04-0110566610.3390/antiox10050666Antiplatelet Activity of Isorhamnetin via Mitochondrial RegulationLyanne Rodríguez0Lina Badimon1Diego Méndez2Teresa Padró3Gemma Vilahur4Esther Peña5Basilio Carrasco6Hermine Vogel7Iván Palomo8Eduardo Fuentes9Thrombosis Research Center, Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, ChileCardiovascular Program—ICCC and CiberCV, IR-Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, SpainThrombosis Research Center, Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, ChileCardiovascular Program—ICCC and CiberCV, IR-Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, SpainCardiovascular Program—ICCC and CiberCV, IR-Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, SpainCardiovascular Program—ICCC and CiberCV, IR-Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, SpainCentro de Estudios en Alimentos Procesados, Talca 3460000, ChileCENATIV, Departamento de Horticultura, Facultad de Ciencias Agrarias, Universidad de Talca, Talca 3460000, ChileThrombosis Research Center, Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, ChileThrombosis Research Center, Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, Medical Technology School, Universidad de Talca, Talca 3460000, ChileWith the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to maintaining good cardiovascular health. In this work, we evaluate the flavonol isorhamnetin’s antiplatelet effect on human platelets, using collagen, thrombin receptor activator peptide 6 (TRAP-6), and phorbol myristate acetate (PMA) as agonists. Isorhamnetin induced a significant inhibition on collagen- and TRAP-6-induced platelet aggregation, with half-maximum inhibitory concentration (IC<sub>50</sub>) values of 8.1 ± 2.6 and 16.1 ± 11.1 µM, respectively; while it did not show cytotoxic effect. Isorhamnetin reduced adenosine triphosphate levels (ATP) in platelets stimulated by collagen and TRAP-6. We also evidenced that isorhamnetin’s antiplatelet activity was related to the inhibition of mitochondrial function without effect on reactive oxygen species (ROS) levels. Additionally, we investigated isorhamnetin’s effect on thrombus formation in vitro under flow conditions on the damaged vessel wall. In this context, we demonstrate that isorhamnetin at 20 µM induced a significant inhibition on platelet deposition, confirming its antithrombotic effect. Our findings corroborate the antiplatelet and antithrombotic potential of isorhamnetin present in many foods of daily consumption.https://www.mdpi.com/2076-3921/10/5/666isorhamnetinflavonoidsantithromboticantiplateletfoods |
| spellingShingle | Lyanne Rodríguez Lina Badimon Diego Méndez Teresa Padró Gemma Vilahur Esther Peña Basilio Carrasco Hermine Vogel Iván Palomo Eduardo Fuentes Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation Antioxidants isorhamnetin flavonoids antithrombotic antiplatelet foods |
| title | Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation |
| title_full | Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation |
| title_fullStr | Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation |
| title_full_unstemmed | Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation |
| title_short | Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation |
| title_sort | antiplatelet activity of isorhamnetin via mitochondrial regulation |
| topic | isorhamnetin flavonoids antithrombotic antiplatelet foods |
| url | https://www.mdpi.com/2076-3921/10/5/666 |
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