Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice
Duchenne muscular dystrophy (DMD) and murine X-linked muscular dystrophy (mdx), its murine model, are characterized by muscle damage and muscle weakness associated with inflammation and new vessel formation. Caveolins, dystrophin-associated proteins, are involved in the pathogenesis of DMD, because...
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| Format: | Article |
| Language: | English |
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Via Medica
2011-07-01
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| Series: | Folia Histochemica et Cytobiologica |
| Subjects: | |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4127 |
| _version_ | 1811336809678897152 |
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| author | Alexander Spassov Tomasz Gedrange Katarzyna Sporniak-Tutak Agnieszka Mielczarek Sven Morgenstern Silke Lucke Tomasz Gredes Christiane Kunert-Keil |
| author_facet | Alexander Spassov Tomasz Gedrange Katarzyna Sporniak-Tutak Agnieszka Mielczarek Sven Morgenstern Silke Lucke Tomasz Gredes Christiane Kunert-Keil |
| author_sort | Alexander Spassov |
| collection | DOAJ |
| description | Duchenne muscular dystrophy (DMD) and murine X-linked muscular dystrophy (mdx), its murine model, are characterized by muscle damage and muscle weakness associated with inflammation and new vessel formation. Caveolins, dystrophin-associated proteins, are involved in the pathogenesis of DMD, because increased numbers of caveolae are found in DMD and mdx hindlimb muscles. Caveolae influence angiogenesis due to their content of vascular endothelial growth factor (VEGF) receptors. Orofacial muscles in mdx mice undergo muscle necrosis followed by muscle regeneration. To ascertain the role of caveolins and VEGF in the pathogenesis of dystrophic masticatory muscles, we examined the expression of caveolin-1 (cav-1), caveolin-3 (cav-3) and VEGF in control and mdx mice. In mdx masticatory muscles, no changes in transcript and protein levels of VEGF were found, whereas cav-1 and cav-3 expression was increased. Using immunohistochemistry, a strong sarcolemmal staining of caveolin-3 in regenerated muscle fibers was found. Furthermore, immunohistochemistry with the caveolin-1 antibody showed an increase in the amount of blood vessels in areas with regenerating muscle fibers. Dystrophic masticatory muscles showed changes comparable to those of hindlimb muscles in the expression of cav-1 and cav-3. The angiogenesis seems to be unaffected in the jaw muscles of mdx mice. We speculate that the increased caveolin expression could cause extensive and efficient muscle regeneration. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 291–298) |
| first_indexed | 2024-04-13T17:45:59Z |
| format | Article |
| id | doaj.art-4d20aa8dac9549bd9d94532869bbeea5 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-04-13T17:45:59Z |
| publishDate | 2011-07-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-4d20aa8dac9549bd9d94532869bbeea52022-12-22T02:36:59ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312011-07-0149229129810.5603/4127Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx miceAlexander SpassovTomasz GedrangeKatarzyna Sporniak-TutakAgnieszka MielczarekSven MorgensternSilke LuckeTomasz GredesChristiane Kunert-KeilDuchenne muscular dystrophy (DMD) and murine X-linked muscular dystrophy (mdx), its murine model, are characterized by muscle damage and muscle weakness associated with inflammation and new vessel formation. Caveolins, dystrophin-associated proteins, are involved in the pathogenesis of DMD, because increased numbers of caveolae are found in DMD and mdx hindlimb muscles. Caveolae influence angiogenesis due to their content of vascular endothelial growth factor (VEGF) receptors. Orofacial muscles in mdx mice undergo muscle necrosis followed by muscle regeneration. To ascertain the role of caveolins and VEGF in the pathogenesis of dystrophic masticatory muscles, we examined the expression of caveolin-1 (cav-1), caveolin-3 (cav-3) and VEGF in control and mdx mice. In mdx masticatory muscles, no changes in transcript and protein levels of VEGF were found, whereas cav-1 and cav-3 expression was increased. Using immunohistochemistry, a strong sarcolemmal staining of caveolin-3 in regenerated muscle fibers was found. Furthermore, immunohistochemistry with the caveolin-1 antibody showed an increase in the amount of blood vessels in areas with regenerating muscle fibers. Dystrophic masticatory muscles showed changes comparable to those of hindlimb muscles in the expression of cav-1 and cav-3. The angiogenesis seems to be unaffected in the jaw muscles of mdx mice. We speculate that the increased caveolin expression could cause extensive and efficient muscle regeneration. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 291–298)http://czasopisma.viamedica.pl/fhc/article/view/4127mdx micemuscular dystrophyhistopathologymasticatory muscles |
| spellingShingle | Alexander Spassov Tomasz Gedrange Katarzyna Sporniak-Tutak Agnieszka Mielczarek Sven Morgenstern Silke Lucke Tomasz Gredes Christiane Kunert-Keil Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice Folia Histochemica et Cytobiologica mdx mice muscular dystrophy histopathology masticatory muscles |
| title | Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice |
| title_full | Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice |
| title_fullStr | Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice |
| title_full_unstemmed | Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice |
| title_short | Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice |
| title_sort | caveolin 1 caveolin 3 and vegf expression in the masticatory muscles of mdx mice |
| topic | mdx mice muscular dystrophy histopathology masticatory muscles |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4127 |
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