Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy
Italy holds important genetic resources of small ruminant breeds. By distinguishing goat breeds at the DNA level, certification of products from specific breeds can be valorized. The aim of this study was to establish the genetic identity of Facciuta della Valnerina, a local goat population of Italy...
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/601 |
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| author | Simone Ceccobelli Emiliano Lasagna Eymen Demir Giacomo Rovelli Emidio Albertini Fabio Veronesi Francesca Maria Sarti Daniele Rosellini |
| author_facet | Simone Ceccobelli Emiliano Lasagna Eymen Demir Giacomo Rovelli Emidio Albertini Fabio Veronesi Francesca Maria Sarti Daniele Rosellini |
| author_sort | Simone Ceccobelli |
| collection | DOAJ |
| description | Italy holds important genetic resources of small ruminant breeds. By distinguishing goat breeds at the DNA level, certification of products from specific breeds can be valorized. The aim of this study was to establish the genetic identity of Facciuta della Valnerina, a local goat population of Italy, compared with the cosmopolitan breeds, Saanen and Camosciata delle Alpi, reared in the same geographic area. A total of 116 microsatellite alleles ranging from 4 to 13 were detected at 16 loci in the three goat populations/breeds. A total of 23 private alleles with frequencies lower than 0.3 were detected in the Facciuta della Valnerina population. The mean numbers of alleles were 6.67, 4.58, and 4.92 in Facciuta della Valnerina, Camosciata delle Alpi, and Saanen, respectively. The expected heterozygosity ranged from 0.20 to 0.86. Most loci were highly polymorphic and informative (polymorphic information content ≥0.50). Factorial correspondence analysis and principal components analysis revealed very clear separation between Facciuta della Valnerina and the two reference goat breeds. Reducing the number of markers from 16 to 12 (on the basis of polymorphic information content and the number of alleles) still allowed us to distinguish the local population, indicating that microsatellite markers are capable of discriminating local livestock breeds at a low cost. |
| first_indexed | 2024-03-10T20:44:48Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:44:48Z |
| publishDate | 2020-04-01 |
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| series | Animals |
| spelling | doaj.art-4d22d3c03f55420dab862a10ada0e9aa2023-11-19T20:24:08ZengMDPI AGAnimals2076-26152020-04-0110460110.3390/ani10040601Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, ItalySimone Ceccobelli0Emiliano Lasagna1Eymen Demir2Giacomo Rovelli3Emidio Albertini4Fabio Veronesi5Francesca Maria Sarti6Daniele Rosellini7Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX giugno 74, 06121, ItalyItaly holds important genetic resources of small ruminant breeds. By distinguishing goat breeds at the DNA level, certification of products from specific breeds can be valorized. The aim of this study was to establish the genetic identity of Facciuta della Valnerina, a local goat population of Italy, compared with the cosmopolitan breeds, Saanen and Camosciata delle Alpi, reared in the same geographic area. A total of 116 microsatellite alleles ranging from 4 to 13 were detected at 16 loci in the three goat populations/breeds. A total of 23 private alleles with frequencies lower than 0.3 were detected in the Facciuta della Valnerina population. The mean numbers of alleles were 6.67, 4.58, and 4.92 in Facciuta della Valnerina, Camosciata delle Alpi, and Saanen, respectively. The expected heterozygosity ranged from 0.20 to 0.86. Most loci were highly polymorphic and informative (polymorphic information content ≥0.50). Factorial correspondence analysis and principal components analysis revealed very clear separation between Facciuta della Valnerina and the two reference goat breeds. Reducing the number of markers from 16 to 12 (on the basis of polymorphic information content and the number of alleles) still allowed us to distinguish the local population, indicating that microsatellite markers are capable of discriminating local livestock breeds at a low cost.https://www.mdpi.com/2076-2615/10/4/601animal biodiversity<i>Capra hircus</i>genetic distinctivenessmicrosatellite markers, molecular traceabilitySSR |
| spellingShingle | Simone Ceccobelli Emiliano Lasagna Eymen Demir Giacomo Rovelli Emidio Albertini Fabio Veronesi Francesca Maria Sarti Daniele Rosellini Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy Animals animal biodiversity <i>Capra hircus</i> genetic distinctiveness microsatellite markers, molecular traceability SSR |
| title | Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy |
| title_full | Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy |
| title_fullStr | Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy |
| title_full_unstemmed | Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy |
| title_short | Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy |
| title_sort | molecular identification of the facciuta della valnerina local goat population reared in the umbria region italy |
| topic | animal biodiversity <i>Capra hircus</i> genetic distinctiveness microsatellite markers, molecular traceability SSR |
| url | https://www.mdpi.com/2076-2615/10/4/601 |
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