Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration
Abstract In this study, dynamic changes in structural polysaccharide deposition on the plasma membrane and cortical microtubules (CMTs) behavior were monitored in protoplasts isolated from white birch callus using confocal laser scanning microscopy and atomic force microscopy. We focused on the infl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-09-01
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| Series: | Journal of Wood Science |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s10086-019-1826-0 |
| _version_ | 1818113786837467136 |
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| author | Satomi Tagawa Yusuke Yamagishi Ugai Watanabe Ryo Funada Tetsuo Kondo |
| author_facet | Satomi Tagawa Yusuke Yamagishi Ugai Watanabe Ryo Funada Tetsuo Kondo |
| author_sort | Satomi Tagawa |
| collection | DOAJ |
| description | Abstract In this study, dynamic changes in structural polysaccharide deposition on the plasma membrane and cortical microtubules (CMTs) behavior were monitored in protoplasts isolated from white birch callus using confocal laser scanning microscopy and atomic force microscopy. We focused on the influence of an environmental stimulus on cell wall regeneration in protoplasts by employing an acidic culture medium containing a high concentration of Ca2+ (the stress condition). Under the non-stress condition, cellulose microfibrils and callose were initially synthesized, and thereafter deposited on the plasma membrane as “primary cell wall material”. Under the stress condition, callose micro-sized fibers were secreted without cell wall regeneration. Behavior of CMTs labeled with mammalian microtubule-associated protein 4 with green fluorescent protein in transgenic protoplasts was monitored by time-lapse video analysis. Under the non-stress condition, CMTs behavior showed a linear arrangement at a fixed position, whereas unfixed manner of CMTs behavior was observed under the stress condition. These findings indicate that excessive Ca2+ affects cellulose synthesis and CMTs dynamics in plant protoplasts. Current study first demonstrated dynamics of cell wall regeneration and CMTs in woody protoplast, which provides novel insight to aid in understanding early stages of primary cell wall formation in plants. |
| first_indexed | 2024-12-11T03:40:22Z |
| format | Article |
| id | doaj.art-4d2654ee062a48db8676bb58e77bfec1 |
| institution | Directory Open Access Journal |
| issn | 1435-0211 1611-4663 |
| language | English |
| last_indexed | 2024-12-11T03:40:22Z |
| publishDate | 2019-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Wood Science |
| spelling | doaj.art-4d2654ee062a48db8676bb58e77bfec12022-12-22T01:22:09ZengSpringerOpenJournal of Wood Science1435-02111611-46632019-09-0165111010.1186/s10086-019-1826-0Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regenerationSatomi Tagawa0Yusuke Yamagishi1Ugai Watanabe2Ryo Funada3Tetsuo Kondo4Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu UniversityResearch Faculty of Agriculture, Hokkaido UniversityFaculty of Advanced Engineering, Chiba Institute of TechnologyFaculty of Agriculture, Tokyo University of Agriculture and TechnologyGraduate School of Bioresource and Bioenvironmental Sciences, Kyushu UniversityAbstract In this study, dynamic changes in structural polysaccharide deposition on the plasma membrane and cortical microtubules (CMTs) behavior were monitored in protoplasts isolated from white birch callus using confocal laser scanning microscopy and atomic force microscopy. We focused on the influence of an environmental stimulus on cell wall regeneration in protoplasts by employing an acidic culture medium containing a high concentration of Ca2+ (the stress condition). Under the non-stress condition, cellulose microfibrils and callose were initially synthesized, and thereafter deposited on the plasma membrane as “primary cell wall material”. Under the stress condition, callose micro-sized fibers were secreted without cell wall regeneration. Behavior of CMTs labeled with mammalian microtubule-associated protein 4 with green fluorescent protein in transgenic protoplasts was monitored by time-lapse video analysis. Under the non-stress condition, CMTs behavior showed a linear arrangement at a fixed position, whereas unfixed manner of CMTs behavior was observed under the stress condition. These findings indicate that excessive Ca2+ affects cellulose synthesis and CMTs dynamics in plant protoplasts. Current study first demonstrated dynamics of cell wall regeneration and CMTs in woody protoplast, which provides novel insight to aid in understanding early stages of primary cell wall formation in plants.http://link.springer.com/article/10.1186/s10086-019-1826-0Plant protoplastsCalloseCell wall formationStress-responseCortical microtubules |
| spellingShingle | Satomi Tagawa Yusuke Yamagishi Ugai Watanabe Ryo Funada Tetsuo Kondo Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration Journal of Wood Science Plant protoplasts Callose Cell wall formation Stress-response Cortical microtubules |
| title | Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration |
| title_full | Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration |
| title_fullStr | Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration |
| title_full_unstemmed | Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration |
| title_short | Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration |
| title_sort | dynamics of structural polysaccharides deposition on the plasma membrane surface of plant protoplasts during cell wall regeneration |
| topic | Plant protoplasts Callose Cell wall formation Stress-response Cortical microtubules |
| url | http://link.springer.com/article/10.1186/s10086-019-1826-0 |
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