Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins
Fifty-five clinical and environmental Aspergillus fumigatus isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability w...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000300005 |
| _version_ | 1797718539192238080 |
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| author | Esperanza Duarte-Escalante Gerardo Zúñiga Oscar Nava Ramírez Susana Córdoba Nicolás Refojo Roberto Arenas Laurence Delhaes María del Rocío Reyes-Montes |
| author_facet | Esperanza Duarte-Escalante Gerardo Zúñiga Oscar Nava Ramírez Susana Córdoba Nicolás Refojo Roberto Arenas Laurence Delhaes María del Rocío Reyes-Montes |
| author_sort | Esperanza Duarte-Escalante |
| collection | DOAJ |
| description | Fifty-five clinical and environmental Aspergillus fumigatus isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (I A). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The I A of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin. |
| first_indexed | 2024-03-12T08:51:48Z |
| format | Article |
| id | doaj.art-4d26cc7c59d942d9aecf6c821713979c |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:48Z |
| publishDate | 2009-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-4d26cc7c59d942d9aecf6c821713979c2023-09-02T16:17:41ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-05-01104342743310.1590/S0074-02762009000300005Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic originsEsperanza Duarte-EscalanteGerardo ZúñigaOscar Nava RamírezSusana CórdobaNicolás RefojoRoberto ArenasLaurence DelhaesMaría del Rocío Reyes-MontesFifty-five clinical and environmental Aspergillus fumigatus isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (I A). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The I A of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000300005Aspergillus fumigatusAFLPgenetic diversitypolymorphisms |
| spellingShingle | Esperanza Duarte-Escalante Gerardo Zúñiga Oscar Nava Ramírez Susana Córdoba Nicolás Refojo Roberto Arenas Laurence Delhaes María del Rocío Reyes-Montes Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins Memorias do Instituto Oswaldo Cruz Aspergillus fumigatus AFLP genetic diversity polymorphisms |
| title | Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins |
| title_full | Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins |
| title_fullStr | Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins |
| title_full_unstemmed | Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins |
| title_short | Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins |
| title_sort | population structure and diversity of the pathogenic fungus aspergillus fumigatus isolated from different sources and geographic origins |
| topic | Aspergillus fumigatus AFLP genetic diversity polymorphisms |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000300005 |
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