Loss of Cep72 affects the morphology of spermatozoa in mice
The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identif...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.948965/full |
| _version_ | 1811227212233310208 |
|---|---|
| author | Zhen Chen Yating Xu Dupeng Ma Changrong Li Ziqi Yu Cong Liu Tingyu Jin Ziye Du Zejia Li Qi Sun Yumin Xu Rong Liu Yuerong Wu Mengcheng Luo |
| author_facet | Zhen Chen Yating Xu Dupeng Ma Changrong Li Ziqi Yu Cong Liu Tingyu Jin Ziye Du Zejia Li Qi Sun Yumin Xu Rong Liu Yuerong Wu Mengcheng Luo |
| author_sort | Zhen Chen |
| collection | DOAJ |
| description | The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identify Cep72 as a testis-specific expression protein. Although Cep72 knockout mice were viable and fertile, their sperms were morphologically abnormal with incomplete flagellum structures. Transcriptome analysis reveals significant differences in six genes (Gm49527, Hbb-bt, Hba-a2, Rps27a-ps2, Gm29647, and Gm8430), which were not previously associated with spermatogenesis. Overall, these results indicate that Cep72 participates in regulating sperm morphology and yet is dispensable for fertility in mice. |
| first_indexed | 2024-04-12T09:38:45Z |
| format | Article |
| id | doaj.art-4d2b1cd1ebd64348bdbd599f51e3a348 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T09:38:45Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-4d2b1cd1ebd64348bdbd599f51e3a3482022-12-22T03:38:09ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-10-011310.3389/fphys.2022.948965948965Loss of Cep72 affects the morphology of spermatozoa in miceZhen Chen0Yating Xu1Dupeng Ma2Changrong Li3Ziqi Yu4Cong Liu5Tingyu Jin6Ziye Du7Zejia Li8Qi Sun9Yumin Xu10Rong Liu11Yuerong Wu12Mengcheng Luo13Hubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaCenter for Animal Experiment, Wuhan University, Wuhan, ChinaHubei Provincial Key Laboratory of Developmentally Originated Disease, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, ChinaThe centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identify Cep72 as a testis-specific expression protein. Although Cep72 knockout mice were viable and fertile, their sperms were morphologically abnormal with incomplete flagellum structures. Transcriptome analysis reveals significant differences in six genes (Gm49527, Hbb-bt, Hba-a2, Rps27a-ps2, Gm29647, and Gm8430), which were not previously associated with spermatogenesis. Overall, these results indicate that Cep72 participates in regulating sperm morphology and yet is dispensable for fertility in mice.https://www.frontiersin.org/articles/10.3389/fphys.2022.948965/fullCep72centrosomespermiogenesisfertilitysperm flagellum |
| spellingShingle | Zhen Chen Yating Xu Dupeng Ma Changrong Li Ziqi Yu Cong Liu Tingyu Jin Ziye Du Zejia Li Qi Sun Yumin Xu Rong Liu Yuerong Wu Mengcheng Luo Loss of Cep72 affects the morphology of spermatozoa in mice Frontiers in Physiology Cep72 centrosome spermiogenesis fertility sperm flagellum |
| title | Loss of Cep72 affects the morphology of spermatozoa in mice |
| title_full | Loss of Cep72 affects the morphology of spermatozoa in mice |
| title_fullStr | Loss of Cep72 affects the morphology of spermatozoa in mice |
| title_full_unstemmed | Loss of Cep72 affects the morphology of spermatozoa in mice |
| title_short | Loss of Cep72 affects the morphology of spermatozoa in mice |
| title_sort | loss of cep72 affects the morphology of spermatozoa in mice |
| topic | Cep72 centrosome spermiogenesis fertility sperm flagellum |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.948965/full |
| work_keys_str_mv | AT zhenchen lossofcep72affectsthemorphologyofspermatozoainmice AT yatingxu lossofcep72affectsthemorphologyofspermatozoainmice AT dupengma lossofcep72affectsthemorphologyofspermatozoainmice AT changrongli lossofcep72affectsthemorphologyofspermatozoainmice AT ziqiyu lossofcep72affectsthemorphologyofspermatozoainmice AT congliu lossofcep72affectsthemorphologyofspermatozoainmice AT tingyujin lossofcep72affectsthemorphologyofspermatozoainmice AT ziyedu lossofcep72affectsthemorphologyofspermatozoainmice AT zejiali lossofcep72affectsthemorphologyofspermatozoainmice AT qisun lossofcep72affectsthemorphologyofspermatozoainmice AT yuminxu lossofcep72affectsthemorphologyofspermatozoainmice AT rongliu lossofcep72affectsthemorphologyofspermatozoainmice AT yuerongwu lossofcep72affectsthemorphologyofspermatozoainmice AT mengchengluo lossofcep72affectsthemorphologyofspermatozoainmice |