Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection
A rapid and high-throughput fluorescence detection method for zearalenone (ZEN) based on a CuO nanoparticle (NP)-assisted signal amplification immunosensor was developed using an automated sample pretreatment and signal conversion system. CuO NPs with high stability and biocompatibility were used as...
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MDPI AG
2021-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/19/6538 |
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| author | Zhihong Xuan Yanxiang Wu Hongmei Liu Li Li Jin Ye Songxue Wang |
| author_facet | Zhihong Xuan Yanxiang Wu Hongmei Liu Li Li Jin Ye Songxue Wang |
| author_sort | Zhihong Xuan |
| collection | DOAJ |
| description | A rapid and high-throughput fluorescence detection method for zearalenone (ZEN) based on a CuO nanoparticle (NP)-assisted signal amplification immunosensor was developed using an automated sample pretreatment and signal conversion system. CuO NPs with high stability and biocompatibility were used as carriers to immobilize anti-ZEN antibodies. The obtained CuO NP-anti-ZEN can maintain the ability to recognize target toxins and act as both a signal source and carrier to achieve signal conversion using automated equipment. In this process, target toxin detection is indirectly transformed to Cu<sup>2+</sup> detection because of the large number of Cu<sup>2+</sup> ions released from CuO NPs under acidic conditions. Finally, a simple and high-throughput fluorescence assay based on a fluorescent tripeptide molecule was employed to detect Cu<sup>2+</sup>, using a multifunctional microporous plate detector. A good linear relationship was observed between the fluorescence signal and the logarithm of ZEN concentration in the range of 16.0–1600.0 μg/kg. Additionally, excellent accuracy with a high recovery yield of 99.2–104.9% was obtained, which was concordant with the results obtained from LC-MS/MS of naturally contaminated samples. The CuO NP-based assay is a powerful and efficient screening tool for ZEN detection and can easily be modified to detect other mycotoxins. |
| first_indexed | 2024-03-10T06:51:01Z |
| format | Article |
| id | doaj.art-4d2c88832bf0447c8008bf718a0ad258 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T06:51:01Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-4d2c88832bf0447c8008bf718a0ad2582023-11-22T16:47:37ZengMDPI AGSensors1424-82202021-09-012119653810.3390/s21196538Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal DetectionZhihong Xuan0Yanxiang Wu1Hongmei Liu2Li Li3Jin Ye4Songxue Wang5Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, ChinaAcademy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, ChinaA rapid and high-throughput fluorescence detection method for zearalenone (ZEN) based on a CuO nanoparticle (NP)-assisted signal amplification immunosensor was developed using an automated sample pretreatment and signal conversion system. CuO NPs with high stability and biocompatibility were used as carriers to immobilize anti-ZEN antibodies. The obtained CuO NP-anti-ZEN can maintain the ability to recognize target toxins and act as both a signal source and carrier to achieve signal conversion using automated equipment. In this process, target toxin detection is indirectly transformed to Cu<sup>2+</sup> detection because of the large number of Cu<sup>2+</sup> ions released from CuO NPs under acidic conditions. Finally, a simple and high-throughput fluorescence assay based on a fluorescent tripeptide molecule was employed to detect Cu<sup>2+</sup>, using a multifunctional microporous plate detector. A good linear relationship was observed between the fluorescence signal and the logarithm of ZEN concentration in the range of 16.0–1600.0 μg/kg. Additionally, excellent accuracy with a high recovery yield of 99.2–104.9% was obtained, which was concordant with the results obtained from LC-MS/MS of naturally contaminated samples. The CuO NP-based assay is a powerful and efficient screening tool for ZEN detection and can easily be modified to detect other mycotoxins.https://www.mdpi.com/1424-8220/21/19/6538zearalenonehigh-throughput pre-treatmentCuO NPsautomatic signal conversion |
| spellingShingle | Zhihong Xuan Yanxiang Wu Hongmei Liu Li Li Jin Ye Songxue Wang Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection Sensors zearalenone high-throughput pre-treatment CuO NPs automatic signal conversion |
| title | Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection |
| title_full | Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection |
| title_fullStr | Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection |
| title_full_unstemmed | Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection |
| title_short | Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection |
| title_sort | copper oxide nanoparticle based immunosensor for zearalenone analysis by combining automated sample pre processing and high throughput terminal detection |
| topic | zearalenone high-throughput pre-treatment CuO NPs automatic signal conversion |
| url | https://www.mdpi.com/1424-8220/21/19/6538 |
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