Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach
Owing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization proce...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/2/259 |
| _version_ | 1818501548934692864 |
|---|---|
| author | Tássia Silva Tavares Eduardo Pereira da Rocha Francisco Guilherme Esteves Nogueira Juliana Arriel Torres Maria Cristina Silva Kamil Kuca Teodorico C. Ramalho |
| author_facet | Tássia Silva Tavares Eduardo Pereira da Rocha Francisco Guilherme Esteves Nogueira Juliana Arriel Torres Maria Cristina Silva Kamil Kuca Teodorico C. Ramalho |
| author_sort | Tássia Silva Tavares |
| collection | DOAJ |
| description | Owing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization process on Δ-FeOOH nanoparticles (NPs). The enzyme/NPs ratio (X1), pH (X2), temperature (X3), and time (X4) were the independent variables analyzed, and immobilized enzyme activity was the response variable (Y). The effects of the factors were studied using a factorial design at two levels (−1 and 1). The biocatalyst obtained was evaluated for the ferulic acid (FA) removal, a pollutant model. The materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The SEM images indicated changes in material morphology. The independent variables X1 (−0.57), X2 (0.71), and X4 (0.42) presented the significance effects estimate. The variable combinations resulted in two significance effects estimates, X1*X2 (−0.57) and X2*X4 (0.39). The immobilized HRP by optimized conditions (X1 = 1/63 (enzyme/NPs ratio, X2 = pH 8, X4 = 60 °C, and 30 min) showed high efficiency for FA oxidation (82%). |
| first_indexed | 2024-12-10T20:57:44Z |
| format | Article |
| id | doaj.art-4d330a6f9e2a444c8e48f8d5f4208a0e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T20:57:44Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4d330a6f9e2a444c8e48f8d5f4208a0e2022-12-22T01:33:55ZengMDPI AGMolecules1420-30492020-01-0125225910.3390/molecules25020259molecules25020259Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric ApproachTássia Silva Tavares0Eduardo Pereira da Rocha1Francisco Guilherme Esteves Nogueira2Juliana Arriel Torres3Maria Cristina Silva4Kamil Kuca5Teodorico C. Ramalho6Department of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, BrazilDepartment of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, BrazilDepartment of Chemistry Engineering, Federal University of São Carlos, 13565-905 São Carlos, SP, BrazilDepartment of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, BrazilDepartment of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, BrazilDepartment of Chemistry, Faculty of Science, University of Hradec Králové, 500 03 Hradec Králové, Czech RepublicDepartment of Chemistry, Federal University of Lavras, N° 37, Lavras, MG 37200-000, BrazilOwing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization process on Δ-FeOOH nanoparticles (NPs). The enzyme/NPs ratio (X1), pH (X2), temperature (X3), and time (X4) were the independent variables analyzed, and immobilized enzyme activity was the response variable (Y). The effects of the factors were studied using a factorial design at two levels (−1 and 1). The biocatalyst obtained was evaluated for the ferulic acid (FA) removal, a pollutant model. The materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The SEM images indicated changes in material morphology. The independent variables X1 (−0.57), X2 (0.71), and X4 (0.42) presented the significance effects estimate. The variable combinations resulted in two significance effects estimates, X1*X2 (−0.57) and X2*X4 (0.39). The immobilized HRP by optimized conditions (X1 = 1/63 (enzyme/NPs ratio, X2 = pH 8, X4 = 60 °C, and 30 min) showed high efficiency for FA oxidation (82%).https://www.mdpi.com/1420-3049/25/2/259immobilizationbioremediationhorseradish peroxidaseiron oxide hydroxide |
| spellingShingle | Tássia Silva Tavares Eduardo Pereira da Rocha Francisco Guilherme Esteves Nogueira Juliana Arriel Torres Maria Cristina Silva Kamil Kuca Teodorico C. Ramalho Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach Molecules immobilization bioremediation horseradish peroxidase iron oxide hydroxide |
| title | Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach |
| title_full | Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach |
| title_fullStr | Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach |
| title_full_unstemmed | Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach |
| title_short | Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach |
| title_sort | δ feooh as support for immobilization peroxidase optimization via a chemometric approach |
| topic | immobilization bioremediation horseradish peroxidase iron oxide hydroxide |
| url | https://www.mdpi.com/1420-3049/25/2/259 |
| work_keys_str_mv | AT tassiasilvatavares dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach AT eduardopereiradarocha dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach AT franciscoguilhermeestevesnogueira dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach AT julianaarrieltorres dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach AT mariacristinasilva dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach AT kamilkuca dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach AT teodoricocramalho dfeoohassupportforimmobilizationperoxidaseoptimizationviaachemometricapproach |