<b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777
By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2012-11-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
| _version_ | 1818859393430585344 |
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| author | Marcelo Maia Pereira Oswaldo Pinto Ribeiro Filho Jose Cola Zanuncio Allan Reis Troni Rodrigo Diana Navarro Angela Emi Takamura |
| author_facet | Marcelo Maia Pereira Oswaldo Pinto Ribeiro Filho Jose Cola Zanuncio Allan Reis Troni Rodrigo Diana Navarro Angela Emi Takamura |
| author_sort | Marcelo Maia Pereira |
| collection | DOAJ |
| description | By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate.
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| first_indexed | 2024-12-19T09:11:28Z |
| format | Article |
| id | doaj.art-4d357c05b68c4b4fa4c9f665f3e010a7 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-19T09:11:28Z |
| publishDate | 2012-11-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-4d357c05b68c4b4fa4c9f665f3e010a72022-12-21T20:28:11ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2012-11-0135110.4025/actascibiolsci.v35i1.14777<b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777Marcelo Maia Pereira0Oswaldo Pinto Ribeiro Filho1Jose Cola Zanuncio2Allan Reis Troni3Rodrigo Diana Navarro4Angela Emi Takamura5Universidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade de BrasíliaUniversidade Federal de ViçosaBy understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling the collection of seven samples of semen, and the first sample was collected 97.5h after the first application. The semen of the creole frog presented the following characteristics: an average volume of 0.38 mL, murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 106 SPTZ mL-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the color, vigor, motility, concentration, and spermatozoa content showed to be adequate. https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777creole frogGnRHasemenspermatozoonsperm motility |
| spellingShingle | Marcelo Maia Pereira Oswaldo Pinto Ribeiro Filho Jose Cola Zanuncio Allan Reis Troni Rodrigo Diana Navarro Angela Emi Takamura <b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777 Acta Scientiarum: Biological Sciences creole frog GnRHa semen spermatozoon sperm motility |
| title | <b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777 |
| title_full | <b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777 |
| title_fullStr | <b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777 |
| title_full_unstemmed | <b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777 |
| title_short | <b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777 |
| title_sort | b buserelin acetate induced spermiation in i leptodactylus ocellatus i and evaluation of semen characteristics b doi 10 4025 actascibiolsci v35i1 14777 |
| topic | creole frog GnRHa semen spermatozoon sperm motility |
| url | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
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