Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns
Extensive burns represent a significant challenge in biomedicine due to the multiple systemic and localized complications resulting from the major skin barrier loss. The functionalization of xenografts with nanostructured antibacterial agents proposes a fast and accessible application to restore bar...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13910 |
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| author | Mario Alberto Pérez-Díaz Elizabeth Alvarado-Gómez María Esther Martínez-Pardo Miguel José Yacamán Andrés Flores-Santos Roberto Sánchez-Sánchez Fidel Martínez-Gutiérrez Horacio Bach |
| author_facet | Mario Alberto Pérez-Díaz Elizabeth Alvarado-Gómez María Esther Martínez-Pardo Miguel José Yacamán Andrés Flores-Santos Roberto Sánchez-Sánchez Fidel Martínez-Gutiérrez Horacio Bach |
| author_sort | Mario Alberto Pérez-Díaz |
| collection | DOAJ |
| description | Extensive burns represent a significant challenge in biomedicine due to the multiple systemic and localized complications resulting from the major skin barrier loss. The functionalization of xenografts with nanostructured antibacterial agents proposes a fast and accessible application to restore barrier function and prevent localized bacterial contamination. Based on this, the objective of this work was to functionalize a xenograft by electrospray deposition with silver nanoparticles (AgNPs) and to evaluate its antibiofilm and cytotoxic effects on human fibroblasts. Initially, AgNPs were synthesized by a green microwave route with sizes of 2.1, 6.8, and 12.2 nm and concentrations of 0.055, 0.167, and 0.500 M, respectively. The AgNPs showed a size relationship directly proportional to the concentration of AgNO<sub>3</sub>, with a spherical and homogeneous distribution determined by high-resolution transmission electron microscopy. The surface functionalization of radiosterilized porcine skin (RPS) via electrospray deposition with the three AgNP concentrations (0.055, 0.167, and 0.500 M) in the epidermis and the dermis showed a uniform distribution on both surfaces by energy-dispersive X-ray spectroscopy. The antibiofilm assays of clinical multidrug-resistant <i>Pseudomonas aeruginosa</i> showed significant effects at the concentrations of 0.167 and 0.500 M, with a log reduction of 1.3 and 2.6, respectively. Additionally, viability experiments with human dermal fibroblasts (HDF) exposed to AgNPs released from functionalized porcine skin showed favorable tolerance, with retention of viability more significant than 90% for concentrations of 0.05 and 0.167 M after 24 h exposure. Antibacterial activity combined with excellent biocompatibility makes this biomaterial a candidate for antibacterial protection by inhibiting bacterial biofilms in deep burns during early stages of development. |
| first_indexed | 2024-03-09T18:17:35Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:17:35Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4d357e7ea07a412890c0ad3ea846d0d52023-11-24T08:35:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221391010.3390/ijms232213910Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep BurnsMario Alberto Pérez-Díaz0Elizabeth Alvarado-Gómez1María Esther Martínez-Pardo2Miguel José Yacamán3Andrés Flores-Santos4Roberto Sánchez-Sánchez5Fidel Martínez-Gutiérrez6Horacio Bach7Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra (INR-LGII), Calzada México Xochimilco No. 289, Colonia Arenal de Guadalupe, Tlalpan, Ciudad de México 14389, MexicoLaboratorio de Antimicrobianos, Biopelículas y Microbiota, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava No. 6, Zona Universitaria, San Luis Potosí 78210, MexicoBanco de Tejidos Radioesterilizados, Instituto Nacional de Investigaciones Nucleares (BTR-ININ), Carretera México-Toluca S/N La Marquesa, Ocoyoacac 52750, MexicoApplied Physics and Materials Science Department, Core Faculty Center for Materials Interfaces in Research and Applications (MIRA), Northern Arizona University, Flagstaff, AZ 86011, USALaboratorio de Antimicrobianos, Biopelículas y Microbiota, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava No. 6, Zona Universitaria, San Luis Potosí 78210, MexicoUnidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra (INR-LGII), Calzada México Xochimilco No. 289, Colonia Arenal de Guadalupe, Tlalpan, Ciudad de México 14389, MexicoLaboratorio de Antimicrobianos, Biopelículas y Microbiota, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava No. 6, Zona Universitaria, San Luis Potosí 78210, MexicoDivision of Infectious Diseases, Department of Medicine, University of British Columbia, 2660 Oak Street, Vancouver, BC V6H 3Z6, CanadaExtensive burns represent a significant challenge in biomedicine due to the multiple systemic and localized complications resulting from the major skin barrier loss. The functionalization of xenografts with nanostructured antibacterial agents proposes a fast and accessible application to restore barrier function and prevent localized bacterial contamination. Based on this, the objective of this work was to functionalize a xenograft by electrospray deposition with silver nanoparticles (AgNPs) and to evaluate its antibiofilm and cytotoxic effects on human fibroblasts. Initially, AgNPs were synthesized by a green microwave route with sizes of 2.1, 6.8, and 12.2 nm and concentrations of 0.055, 0.167, and 0.500 M, respectively. The AgNPs showed a size relationship directly proportional to the concentration of AgNO<sub>3</sub>, with a spherical and homogeneous distribution determined by high-resolution transmission electron microscopy. The surface functionalization of radiosterilized porcine skin (RPS) via electrospray deposition with the three AgNP concentrations (0.055, 0.167, and 0.500 M) in the epidermis and the dermis showed a uniform distribution on both surfaces by energy-dispersive X-ray spectroscopy. The antibiofilm assays of clinical multidrug-resistant <i>Pseudomonas aeruginosa</i> showed significant effects at the concentrations of 0.167 and 0.500 M, with a log reduction of 1.3 and 2.6, respectively. Additionally, viability experiments with human dermal fibroblasts (HDF) exposed to AgNPs released from functionalized porcine skin showed favorable tolerance, with retention of viability more significant than 90% for concentrations of 0.05 and 0.167 M after 24 h exposure. Antibacterial activity combined with excellent biocompatibility makes this biomaterial a candidate for antibacterial protection by inhibiting bacterial biofilms in deep burns during early stages of development.https://www.mdpi.com/1422-0067/23/22/13910antibiofilm activitycytotoxicitynanoparticle depositionmultidrug-resistantmicrowave synthesiselectrospray |
| spellingShingle | Mario Alberto Pérez-Díaz Elizabeth Alvarado-Gómez María Esther Martínez-Pardo Miguel José Yacamán Andrés Flores-Santos Roberto Sánchez-Sánchez Fidel Martínez-Gutiérrez Horacio Bach Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns International Journal of Molecular Sciences antibiofilm activity cytotoxicity nanoparticle deposition multidrug-resistant microwave synthesis electrospray |
| title | Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns |
| title_full | Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns |
| title_fullStr | Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns |
| title_full_unstemmed | Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns |
| title_short | Development of Radiosterilized Porcine Skin Electrosprayed with Silver Nanoparticles Prevents Infections in Deep Burns |
| title_sort | development of radiosterilized porcine skin electrosprayed with silver nanoparticles prevents infections in deep burns |
| topic | antibiofilm activity cytotoxicity nanoparticle deposition multidrug-resistant microwave synthesis electrospray |
| url | https://www.mdpi.com/1422-0067/23/22/13910 |
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