The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats
Background Background: Oxidative stress plays an important role in the development of diabetic complications. Objective This study evaluated the impact of pre- and post-treatment with Lactobacillus acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2019-07-01
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| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/ijrm.v17i7.4861 |
| _version_ | 1818457840548839424 |
|---|---|
| author | Seddigheh Sheikh Hosseini Ali Gol Moje Khaleghi |
| author_facet | Seddigheh Sheikh Hosseini Ali Gol Moje Khaleghi |
| author_sort | Seddigheh Sheikh Hosseini |
| collection | DOAJ |
| description | Background
Background: Oxidative stress plays an important role in the development of diabetic complications.
Objective
This study evaluated the impact of pre- and post-treatment with Lactobacillus acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and epididymis in streptozotocin-induced diabetic rats.
Materials and Methods
Thirty male Wistar rats (10 wk old) weighing 220-230 g. were divided into five groups (n = 6/ each): 1- normal group, 2- normal lactobacillus group, 3- diabetic group, 4- diabetic + lactobacillus before (DLB) group, and 5- diabetic + lactobacillus after (DLA) group. The normal and diabetic groups received daily 1 mL normal saline for 6 wk. Normal lactobacillus group received daily L. acidophilus for 6 wk. Group DLB received daily L. acidophilus for 2 wk before diabetes and for 4 wk after diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes and L. acidophilus for 4 wk after diabetes. The dose of L. acidophilus was 1
×
10
9
CFU/mL.
Results
The administration of L. acidophilus worsened blood glucose level and reduced the levels of Malondialdehyde (p
≤
0.0001) and Hydrogen peroxide (p
≤
0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did not show any changes compared to the diabetic rats. Also, there was no significant difference between DLB and DLA groups, in these markers.
Conclusion
Data obtained suggests that L. acidophilus has anti-oxidant effects on the testis and sometime in the epididymis in diabetic rats. |
| first_indexed | 2024-12-14T22:48:57Z |
| format | Article |
| id | doaj.art-4d364f8bac104c3a967e9e7e4f7dd848 |
| institution | Directory Open Access Journal |
| issn | 2476-3772 |
| language | English |
| last_indexed | 2024-12-14T22:48:57Z |
| publishDate | 2019-07-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-4d364f8bac104c3a967e9e7e4f7dd8482022-12-21T22:44:47ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-37722019-07-011749350210.18502/ijrm.v17i7.4861The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male ratsSeddigheh Sheikh Hosseini0Ali Gol1Moje Khaleghi2Department of Biology, Faculty of Science, University of Shahid Bahonar, Kerman, Iran.Department of Biology, Faculty of Science, University of Shahid Bahonar, Kerman, Iran.Department of Biology, Faculty of Science, University of Shahid Bahonar, Kerman, Iran.Background Background: Oxidative stress plays an important role in the development of diabetic complications. Objective This study evaluated the impact of pre- and post-treatment with Lactobacillus acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and epididymis in streptozotocin-induced diabetic rats. Materials and Methods Thirty male Wistar rats (10 wk old) weighing 220-230 g. were divided into five groups (n = 6/ each): 1- normal group, 2- normal lactobacillus group, 3- diabetic group, 4- diabetic + lactobacillus before (DLB) group, and 5- diabetic + lactobacillus after (DLA) group. The normal and diabetic groups received daily 1 mL normal saline for 6 wk. Normal lactobacillus group received daily L. acidophilus for 6 wk. Group DLB received daily L. acidophilus for 2 wk before diabetes and for 4 wk after diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes and L. acidophilus for 4 wk after diabetes. The dose of L. acidophilus was 1 × 10 9 CFU/mL. Results The administration of L. acidophilus worsened blood glucose level and reduced the levels of Malondialdehyde (p ≤ 0.0001) and Hydrogen peroxide (p ≤ 0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did not show any changes compared to the diabetic rats. Also, there was no significant difference between DLB and DLA groups, in these markers. Conclusion Data obtained suggests that L. acidophilus has anti-oxidant effects on the testis and sometime in the epididymis in diabetic rats.https://doi.org/10.18502/ijrm.v17i7.4861diabetes mellitusoxidative stressratsreproductionlactobacillus acidophilus. |
| spellingShingle | Seddigheh Sheikh Hosseini Ali Gol Moje Khaleghi The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats International Journal of Reproductive BioMedicine diabetes mellitus oxidative stress rats reproduction lactobacillus acidophilus. |
| title | The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats |
| title_full | The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats |
| title_fullStr | The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats |
| title_full_unstemmed | The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats |
| title_short | The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats |
| title_sort | effects of the lactobacillus acidophilus atcc 4356 on the oxidative stress of reproductive system in diabetic male rats |
| topic | diabetes mellitus oxidative stress rats reproduction lactobacillus acidophilus. |
| url | https://doi.org/10.18502/ijrm.v17i7.4861 |
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