Optimal model-based sensorless adaptive optics for epifluorescence microscopy.
We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5860766?pdf=render |
| _version_ | 1818919864304140288 |
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| author | Paolo Pozzi Oleg Soloviev Dean Wilding Gleb Vdovin Michel Verhaegen |
| author_facet | Paolo Pozzi Oleg Soloviev Dean Wilding Gleb Vdovin Michel Verhaegen |
| author_sort | Paolo Pozzi |
| collection | DOAJ |
| description | We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image quality and the aberration. The method is potentially applicable to any form of microscopy with epifluorescence detection, including the practically important case of incoherent fluorescence emission from a three dimensional object, through minor hardware modifications. We have applied the technique successfully to a widefield epifluorescence microscope and to a multiaperture confocal microscope. |
| first_indexed | 2024-12-20T01:12:38Z |
| format | Article |
| id | doaj.art-4d366ac29756479aa0cb7f302e691b9e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:12:38Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4d366ac29756479aa0cb7f302e691b9e2022-12-21T19:58:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019452310.1371/journal.pone.0194523Optimal model-based sensorless adaptive optics for epifluorescence microscopy.Paolo PozziOleg SolovievDean WildingGleb VdovinMichel VerhaegenWe report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image quality and the aberration. The method is potentially applicable to any form of microscopy with epifluorescence detection, including the practically important case of incoherent fluorescence emission from a three dimensional object, through minor hardware modifications. We have applied the technique successfully to a widefield epifluorescence microscope and to a multiaperture confocal microscope.http://europepmc.org/articles/PMC5860766?pdf=render |
| spellingShingle | Paolo Pozzi Oleg Soloviev Dean Wilding Gleb Vdovin Michel Verhaegen Optimal model-based sensorless adaptive optics for epifluorescence microscopy. PLoS ONE |
| title | Optimal model-based sensorless adaptive optics for epifluorescence microscopy. |
| title_full | Optimal model-based sensorless adaptive optics for epifluorescence microscopy. |
| title_fullStr | Optimal model-based sensorless adaptive optics for epifluorescence microscopy. |
| title_full_unstemmed | Optimal model-based sensorless adaptive optics for epifluorescence microscopy. |
| title_short | Optimal model-based sensorless adaptive optics for epifluorescence microscopy. |
| title_sort | optimal model based sensorless adaptive optics for epifluorescence microscopy |
| url | http://europepmc.org/articles/PMC5860766?pdf=render |
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