Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings
Skin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (ε-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/4/441 |
| _version_ | 1797569841826103296 |
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| author | Alan Saúl Álvarez-Suárez Syed G. Dastager Nina Bogdanchikova Daniel Grande Alexey Pestryakov Juan Carlos García-Ramos Graciela Lizeth Pérez-González Karla Juárez-Moreno Yanis Toledano-Magaña Elena Smolentseva Juan Antonio Paz-González Tatiana Popova Lyubov Rachkovskaya Vadim Nimaev Anastasia Kotlyarova Maksim Korolev Andrey Letyagin Luis Jesús Villarreal-Gómez |
| author_facet | Alan Saúl Álvarez-Suárez Syed G. Dastager Nina Bogdanchikova Daniel Grande Alexey Pestryakov Juan Carlos García-Ramos Graciela Lizeth Pérez-González Karla Juárez-Moreno Yanis Toledano-Magaña Elena Smolentseva Juan Antonio Paz-González Tatiana Popova Lyubov Rachkovskaya Vadim Nimaev Anastasia Kotlyarova Maksim Korolev Andrey Letyagin Luis Jesús Villarreal-Gómez |
| author_sort | Alan Saúl Álvarez-Suárez |
| collection | DOAJ |
| description | Skin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (ε-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers produced via electrospinning along with sorbents loaded with Argovit™ silver nanoparticles (Ag-Si/Al<sub>2</sub>O<sub>3</sub>) as constituent components for composite wound dressings. The physicochemical properties of the fibers and sorbents were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The mechanical properties of the fibers were also evaluated. The results of this work showed that the tested fibrous scaffolds have melting temperatures suitable for wound dressings design (58–60 °C). In addition, they demonstrated to be stable even after seven days in physiological solution, showing no macroscopic damage due to PVP release at the microscopic scale. Pelletized sorbents with the higher particle size demonstrated to have the best water uptake capabilities. Both, fibers and sorbents showed antimicrobial activity against Gram-negative bacteria <i>Pseudomona aeruginosa</i> and <i>Escherichia coli</i>, Gram-positive <i>Staphylococcus aureus</i> and the fungus <i>Candida albicans</i>. The best physicochemical properties were obtained with a scaffold produced with a PCL/PVP ratio of 85:15, this polymeric scaffold demonstrated the most antimicrobial activity without affecting the cell viability of human fibroblast. Pelletized Ag/Si-Al<sub>2</sub>O<sub>3</sub>-3 sorbent possessed the best water uptake capability and the higher antimicrobial activity, over time between all the sorbents tested. The combination of PCL/PVP 85:15 microfibers with the chosen Ag/Si-Al<sub>2</sub>O<sub>3</sub>-3 sorbent will be used in the following work for creation of wound dressings possessing exudate retention, biocompatibility and antimicrobial activity. |
| first_indexed | 2024-03-10T20:17:15Z |
| format | Article |
| id | doaj.art-4d39272877394df58e8a8a60ca920b1e |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T20:17:15Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-4d39272877394df58e8a8a60ca920b1e2023-11-19T22:25:39ZengMDPI AGMicromachines2072-666X2020-04-0111444110.3390/mi11040441Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound DressingsAlan Saúl Álvarez-Suárez0Syed G. Dastager1Nina Bogdanchikova2Daniel Grande3Alexey Pestryakov4Juan Carlos García-Ramos5Graciela Lizeth Pérez-González6Karla Juárez-Moreno7Yanis Toledano-Magaña8Elena Smolentseva9Juan Antonio Paz-González10Tatiana Popova11Lyubov Rachkovskaya12Vadim Nimaev13Anastasia Kotlyarova14Maksim Korolev15Andrey Letyagin16Luis Jesús Villarreal-Gómez17Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, MexicoNational Collection of Industrial Microorganisms (NCIM), CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, IndiaUniversidad Nacional Autónoma de México, Centro de Nanociencias y Nanotecnología, Km. 107, Carretera Tijuana a Ensenada, C.P. 22860 Ensenada, Baja California, Mexico“Complex Polymer Systems” Laboratory, Institut de Chimie et des Matériaux Paris-Est, Université Paris-Est Créteil, UMR 7182 CNRS, 2, rue Henri Dunant, F-94320 Thiais, FranceDepartment of Technology of Organic Substances and Polymer Materials, Tomsk Polytechnic University, 634050 Tomsk, RussiaEscuela de Ciencias de la Salud, Universidad Autónoma de Baja California- Campus Valle Dorado, Carretera Transpeninsular S/N, Valle Dorado, 22890 Ensenada, Baja California, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, MexicoUniversidad Nacional Autónoma de México, Centro de Nanociencias y Nanotecnología, Km. 107, Carretera Tijuana a Ensenada, C.P. 22860 Ensenada, Baja California, MexicoEscuela de Ciencias de la Salud, Universidad Autónoma de Baja California- Campus Valle Dorado, Carretera Transpeninsular S/N, Valle Dorado, 22890 Ensenada, Baja California, MexicoUniversidad Nacional Autónoma de México, Centro de Nanociencias y Nanotecnología, Km. 107, Carretera Tijuana a Ensenada, C.P. 22860 Ensenada, Baja California, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, MexicoResearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, RussiaResearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, RussiaResearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, RussiaResearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, RussiaResearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, RussiaResearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630060 Novosibirsk, RussiaFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Valle de las Palmas, Mexico. Blvd. Universitario #1000, Unidad Valle de las Palmas, 22260 Tijuana, Baja California, MexicoSkin burns and ulcers are considered hard-to-heal wounds due to their high infection risk. For this reason, designing new options for wound dressings is a growing need. The objective of this work is to investigate the properties of poly (ε-caprolactone)/poly (vinyl-pyrrolidone) (PCL/PVP) microfibers produced via electrospinning along with sorbents loaded with Argovit™ silver nanoparticles (Ag-Si/Al<sub>2</sub>O<sub>3</sub>) as constituent components for composite wound dressings. The physicochemical properties of the fibers and sorbents were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The mechanical properties of the fibers were also evaluated. The results of this work showed that the tested fibrous scaffolds have melting temperatures suitable for wound dressings design (58–60 °C). In addition, they demonstrated to be stable even after seven days in physiological solution, showing no macroscopic damage due to PVP release at the microscopic scale. Pelletized sorbents with the higher particle size demonstrated to have the best water uptake capabilities. Both, fibers and sorbents showed antimicrobial activity against Gram-negative bacteria <i>Pseudomona aeruginosa</i> and <i>Escherichia coli</i>, Gram-positive <i>Staphylococcus aureus</i> and the fungus <i>Candida albicans</i>. The best physicochemical properties were obtained with a scaffold produced with a PCL/PVP ratio of 85:15, this polymeric scaffold demonstrated the most antimicrobial activity without affecting the cell viability of human fibroblast. Pelletized Ag/Si-Al<sub>2</sub>O<sub>3</sub>-3 sorbent possessed the best water uptake capability and the higher antimicrobial activity, over time between all the sorbents tested. The combination of PCL/PVP 85:15 microfibers with the chosen Ag/Si-Al<sub>2</sub>O<sub>3</sub>-3 sorbent will be used in the following work for creation of wound dressings possessing exudate retention, biocompatibility and antimicrobial activity.https://www.mdpi.com/2072-666X/11/4/441electrospinningpoly (ε-caprolactone)poly (vinyl pyrrolidone)silver sorbentswound dressings |
| spellingShingle | Alan Saúl Álvarez-Suárez Syed G. Dastager Nina Bogdanchikova Daniel Grande Alexey Pestryakov Juan Carlos García-Ramos Graciela Lizeth Pérez-González Karla Juárez-Moreno Yanis Toledano-Magaña Elena Smolentseva Juan Antonio Paz-González Tatiana Popova Lyubov Rachkovskaya Vadim Nimaev Anastasia Kotlyarova Maksim Korolev Andrey Letyagin Luis Jesús Villarreal-Gómez Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings Micromachines electrospinning poly (ε-caprolactone) poly (vinyl pyrrolidone) silver sorbents wound dressings |
| title | Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings |
| title_full | Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings |
| title_fullStr | Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings |
| title_full_unstemmed | Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings |
| title_short | Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings |
| title_sort | electrospun fibers and sorbents as a possible basis for effective composite wound dressings |
| topic | electrospinning poly (ε-caprolactone) poly (vinyl pyrrolidone) silver sorbents wound dressings |
| url | https://www.mdpi.com/2072-666X/11/4/441 |
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