α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots
Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Pharmacological Society
2018-02-01
|
| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/33668 |
| _version_ | 1818364140093177856 |
|---|---|
| author | Songül Karakaya Hilal Özbek Sefa Gözcü Zühal Güvenalp Hafize Yuca Hayri Duman Cavit Kazaz Ceyda Sibel Kiliç |
| author_facet | Songül Karakaya Hilal Özbek Sefa Gözcü Zühal Güvenalp Hafize Yuca Hayri Duman Cavit Kazaz Ceyda Sibel Kiliç |
| author_sort | Songül Karakaya |
| collection | DOAJ |
| description | Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC50 = 4.9 mg/mL). Grandivitinol (IC50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.
Video Clip of Methodology:
8 min 26 sec: Full Screen Alternate |
| first_indexed | 2024-12-13T21:59:38Z |
| format | Article |
| id | doaj.art-4d395db697894e7380557032fe53f529 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-13T21:59:38Z |
| publishDate | 2018-02-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-4d395db697894e7380557032fe53f5292022-12-21T23:30:02ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882018-02-0113110.3329/bjp.v13i1.3366822793α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa rootsSongül Karakaya0Hilal Özbek1Sefa Gözcü2Zühal Güvenalp3Hafize Yuca4Hayri Duman5Cavit Kazaz6Ceyda Sibel Kiliç7Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240Department of Pharmacognosy, Faculty of Pharmacy, Erzincan University, Erzincan 24100Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240Department of Biology, Faculty of Science, Gazi University, Ankara 06500Department of Chemistry, Faculty of Science, Atatürk University, Erzurum 25240Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Ankara 06100Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC50 = 4.9 mg/mL). Grandivitinol (IC50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species. Video Clip of Methodology: 8 min 26 sec: Full Screen Alternatehttps://www.banglajol.info/index.php/BJP/article/view/33668Apiaceaeα-Amylaseα-GlucosidaseCoumarinFerulago blancheanaF. pachyloba |
| spellingShingle | Songül Karakaya Hilal Özbek Sefa Gözcü Zühal Güvenalp Hafize Yuca Hayri Duman Cavit Kazaz Ceyda Sibel Kiliç α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots Bangladesh Journal of Pharmacology Apiaceae α-Amylase α-Glucosidase Coumarin Ferulago blancheana F. pachyloba |
| title | α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots |
| title_full | α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots |
| title_fullStr | α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots |
| title_full_unstemmed | α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots |
| title_short | α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots |
| title_sort | α amylase and α glucosidase inhibitory activities of the extracts and constituents of ferulago blancheana f pachyloba and f trachycarpa roots |
| topic | Apiaceae α-Amylase α-Glucosidase Coumarin Ferulago blancheana F. pachyloba |
| url | https://www.banglajol.info/index.php/BJP/article/view/33668 |
| work_keys_str_mv | AT songulkarakaya aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT hilalozbek aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT sefagozcu aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT zuhalguvenalp aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT hafizeyuca aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT hayriduman aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT cavitkazaz aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots AT ceydasibelkilic aamylaseandaglucosidaseinhibitoryactivitiesoftheextractsandconstituentsofferulagoblancheanafpachylobaandftrachycarparoots |