Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress
Silver nanoparticle (AgNP) production and their use as antimicrobial agents is a current area of active research. Biosynthesis is the most sustainable production method, and fungi have become candidates of interest in AgNP production. However, investigations into the physiological responses of fungi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/5/539 |
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| author | Janelle R. Robinson Omoanghe S. Isikhuemhen Felicia N. Anike Kiran Subedi |
| author_facet | Janelle R. Robinson Omoanghe S. Isikhuemhen Felicia N. Anike Kiran Subedi |
| author_sort | Janelle R. Robinson |
| collection | DOAJ |
| description | Silver nanoparticle (AgNP) production and their use as antimicrobial agents is a current area of active research. Biosynthesis is the most sustainable production method, and fungi have become candidates of interest in AgNP production. However, investigations into the physiological responses of fungi due to silver exposure are scanty. This present work utilized two strains of <i>Saccharomyces cerevisiae</i> (one used in commercial fermentation and a naturally occurring strain) to determine the physiological consequences of their transient exposure to AgNO<sub>3</sub>. The assessments were based on studies involving growth curves, minimal inhibitory concentration assays, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging, and inductively coupled plasma optical emission spectroscopy (ICP-OES). Results indicated (a) the capability of <i>S. cerevisiae</i> to produce silver nanoparticles, even at elevated levels of exposure; (b) strain origin had no significant impact on <i>S. cerevisiae</i> physiological response to AgNO<sub>3</sub>; and (c) coexposure to copper and silver significantly increased intracellular copper, silver, and calcium in treated yeast cells. In addition, electron microscopy and ICP-OES results revealed that both strains internalized silver after exposure, resulting in the shrunken and distorted physical appearance visible on SEM micrographs of treated cells. Though a promising candidate for AgNPs biosynthesis, this study analyzed the effects of transient silver exposure on <i>S. cerevisiae</i> growth physiology and morphology. |
| first_indexed | 2024-03-10T03:36:36Z |
| format | Article |
| id | doaj.art-4d3e9d518f594f619be5d36c70b28654 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T03:36:36Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-4d3e9d518f594f619be5d36c70b286542023-11-23T11:42:33ZengMDPI AGJournal of Fungi2309-608X2022-05-018553910.3390/jof8050539Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver StressJanelle R. Robinson0Omoanghe S. Isikhuemhen1Felicia N. Anike2Kiran Subedi3Department of Natural Resources and Environmental Design, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC 27411, USADepartment of Natural Resources and Environmental Design, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC 27411, USADepartment of Natural Resources and Environmental Design, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC 27411, USAAnalytical Services Laboratory, College of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC 27411, USASilver nanoparticle (AgNP) production and their use as antimicrobial agents is a current area of active research. Biosynthesis is the most sustainable production method, and fungi have become candidates of interest in AgNP production. However, investigations into the physiological responses of fungi due to silver exposure are scanty. This present work utilized two strains of <i>Saccharomyces cerevisiae</i> (one used in commercial fermentation and a naturally occurring strain) to determine the physiological consequences of their transient exposure to AgNO<sub>3</sub>. The assessments were based on studies involving growth curves, minimal inhibitory concentration assays, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging, and inductively coupled plasma optical emission spectroscopy (ICP-OES). Results indicated (a) the capability of <i>S. cerevisiae</i> to produce silver nanoparticles, even at elevated levels of exposure; (b) strain origin had no significant impact on <i>S. cerevisiae</i> physiological response to AgNO<sub>3</sub>; and (c) coexposure to copper and silver significantly increased intracellular copper, silver, and calcium in treated yeast cells. In addition, electron microscopy and ICP-OES results revealed that both strains internalized silver after exposure, resulting in the shrunken and distorted physical appearance visible on SEM micrographs of treated cells. Though a promising candidate for AgNPs biosynthesis, this study analyzed the effects of transient silver exposure on <i>S. cerevisiae</i> growth physiology and morphology.https://www.mdpi.com/2309-608X/8/5/539<i>Saccharomyces cerevisiae</i>silver nitratesilver nanoparticlephysiological responsemycosynthesis |
| spellingShingle | Janelle R. Robinson Omoanghe S. Isikhuemhen Felicia N. Anike Kiran Subedi Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress Journal of Fungi <i>Saccharomyces cerevisiae</i> silver nitrate silver nanoparticle physiological response mycosynthesis |
| title | Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress |
| title_full | Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress |
| title_fullStr | Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress |
| title_full_unstemmed | Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress |
| title_short | Physiological Response of <i>Saccharomyces cerevisiae</i> to Silver Stress |
| title_sort | physiological response of i saccharomyces cerevisiae i to silver stress |
| topic | <i>Saccharomyces cerevisiae</i> silver nitrate silver nanoparticle physiological response mycosynthesis |
| url | https://www.mdpi.com/2309-608X/8/5/539 |
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