Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer
This study aims to determine differences in the on- and off-tumor microbiota between patients with right- and left-sided colorectal cancer. Microbiome profiling of tumor and tumor-adjacent biopsies from patients with right-sided (<i>n</i> = 17) and left-sided (<i>n</i> = 7) c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/5/1108 |
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| author | Oliver Phipps Mohammed N. Quraishi Edward A. Dickson Helen Steed Aditi Kumar Austin G. Acheson Andrew D. Beggs Matthew J. Brookes Hafid Omar Al-Hassi |
| author_facet | Oliver Phipps Mohammed N. Quraishi Edward A. Dickson Helen Steed Aditi Kumar Austin G. Acheson Andrew D. Beggs Matthew J. Brookes Hafid Omar Al-Hassi |
| author_sort | Oliver Phipps |
| collection | DOAJ |
| description | This study aims to determine differences in the on- and off-tumor microbiota between patients with right- and left-sided colorectal cancer. Microbiome profiling of tumor and tumor-adjacent biopsies from patients with right-sided (<i>n</i> = 17) and left-sided (<i>n</i> = 7) colorectal adenocarcinoma was performed using 16S ribosomal RNA sequencing. Off-tumor alpha and beta diversity were significantly different between right- and left-sided colorectal cancer patients. However, no differences in on-tumor diversity were observed between tumor locations. Comparing the off-tumor microbiota showed the right colon to be enriched with species of the Lachnoclostridium, Selenomonas, and Ruminococcus genera. Whereas the left colon is enriched with Epsilonbacteraeota phylum, Campylobacteria class, and Pasteurellales and Campylobacterales orders, in contrast, the on-tumor microbiota showed relatively fewer differences in bacterial taxonomy between tumor sites, with left tumors being enriched with Methylophilaceae and Vadin BE97 families and Alloprevotella, Intestinibacter, Romboutsia, and Ruminococcus 2 genera. Patients with left-sided colorectal cancer had large taxonomic differences between their paired on- and off-tumor microbiota, while patients with right-sided colorectal cancer showed relatively fewer taxonomic differences. Collectively, this suggests that the right and left colon show distinctive bacterial populations; however, the presence of a colonic tumor leads to a more consistent microbiota between locations. |
| first_indexed | 2024-03-10T11:13:08Z |
| format | Article |
| id | doaj.art-4d3edb11b6c64583870fcdde701634a2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:13:08Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-4d3edb11b6c64583870fcdde701634a22023-11-21T20:38:54ZengMDPI AGMicroorganisms2076-26072021-05-0195110810.3390/microorganisms9051108Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal CancerOliver Phipps0Mohammed N. Quraishi1Edward A. Dickson2Helen Steed3Aditi Kumar4Austin G. Acheson5Andrew D. Beggs6Matthew J. Brookes7Hafid Omar Al-Hassi8Research Institute in Healthcare Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKSurgical Research Laboratory, Institute of Cancer & Genomic Science, University of Birmingham, Birmingham B15 2TQ, UKNIHR Biomedical Research Centre in Gastrointestinal and Liver Diseases, Nottingham University Hospitals NHS Trust, Nottingham NG7 2UH, UKResearch Institute in Healthcare Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKResearch Institute in Healthcare Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKNIHR Biomedical Research Centre in Gastrointestinal and Liver Diseases, Nottingham University Hospitals NHS Trust, Nottingham NG7 2UH, UKSurgical Research Laboratory, Institute of Cancer & Genomic Science, University of Birmingham, Birmingham B15 2TQ, UKResearch Institute in Healthcare Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKResearch Institute in Healthcare Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKThis study aims to determine differences in the on- and off-tumor microbiota between patients with right- and left-sided colorectal cancer. Microbiome profiling of tumor and tumor-adjacent biopsies from patients with right-sided (<i>n</i> = 17) and left-sided (<i>n</i> = 7) colorectal adenocarcinoma was performed using 16S ribosomal RNA sequencing. Off-tumor alpha and beta diversity were significantly different between right- and left-sided colorectal cancer patients. However, no differences in on-tumor diversity were observed between tumor locations. Comparing the off-tumor microbiota showed the right colon to be enriched with species of the Lachnoclostridium, Selenomonas, and Ruminococcus genera. Whereas the left colon is enriched with Epsilonbacteraeota phylum, Campylobacteria class, and Pasteurellales and Campylobacterales orders, in contrast, the on-tumor microbiota showed relatively fewer differences in bacterial taxonomy between tumor sites, with left tumors being enriched with Methylophilaceae and Vadin BE97 families and Alloprevotella, Intestinibacter, Romboutsia, and Ruminococcus 2 genera. Patients with left-sided colorectal cancer had large taxonomic differences between their paired on- and off-tumor microbiota, while patients with right-sided colorectal cancer showed relatively fewer taxonomic differences. Collectively, this suggests that the right and left colon show distinctive bacterial populations; however, the presence of a colonic tumor leads to a more consistent microbiota between locations.https://www.mdpi.com/2076-2607/9/5/1108right- and left-sided colorectal cancergut microbiome16S rRNAtumor microbiotaproximal and distal colon |
| spellingShingle | Oliver Phipps Mohammed N. Quraishi Edward A. Dickson Helen Steed Aditi Kumar Austin G. Acheson Andrew D. Beggs Matthew J. Brookes Hafid Omar Al-Hassi Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer Microorganisms right- and left-sided colorectal cancer gut microbiome 16S rRNA tumor microbiota proximal and distal colon |
| title | Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer |
| title_full | Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer |
| title_fullStr | Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer |
| title_full_unstemmed | Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer |
| title_short | Differences in the On- and Off-Tumor Microbiota between Right- and Left-Sided Colorectal Cancer |
| title_sort | differences in the on and off tumor microbiota between right and left sided colorectal cancer |
| topic | right- and left-sided colorectal cancer gut microbiome 16S rRNA tumor microbiota proximal and distal colon |
| url | https://www.mdpi.com/2076-2607/9/5/1108 |
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