In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii
[english] Aim: The in vitro activity of colistin in combination with sulbactam, netilmicin, and vancomycin against colistin-resistant strains was investigated. Furthermore, the clonal relationship of the strains was analyzed. Methods: Clonal relationship was investigated using rep-PCR. To screen fo...
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2014-08-01
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| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | http://www.egms.de/static/en/journals/dgkh/2014-9/dgkh000234.shtml |
| _version_ | 1818132583814266880 |
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| author | Percin, Duygu Akyol, Selcan Kalin, Gamze |
| author_facet | Percin, Duygu Akyol, Selcan Kalin, Gamze |
| author_sort | Percin, Duygu |
| collection | DOAJ |
| description | [english] Aim: The in vitro activity of colistin in combination with sulbactam, netilmicin, and vancomycin against colistin-resistant strains was investigated. Furthermore, the clonal relationship of the strains was analyzed. Methods: Clonal relationship was investigated using rep-PCR. To screen for synergysm, the fractional inhibitory concentration index (FICI) was calculated using checkerboard assay. The killing kinetics of the combination of colistin with vancomycin was assessed using time-kill assay. Results: Three different clones were found among 10 clinical isolates of colistin-resistant strains. Thereof, 8 strains were susceptible to netilmicin. Synergistic interaction was detected in 1 strain with the combination of colistin-netilmicin, in 5 strains with colistin-sulbactam, and in 9 strains with colistin-vancomycin. None of combinations had antagonistic activity. Colistin-vancomycin combination resulted in rapid bactericidal activity. Conclusion: These results show a distinct in vitro synergism between colistin and vancomycin, which might be useful to treat infection with multiple-resistant strains, prevent emergence of resistant strains, and to lower doses for both antibiotics to be used. |
| first_indexed | 2024-12-11T08:39:08Z |
| format | Article |
| id | doaj.art-4d42fc33b0c9499e8ac9541f5ac22f7f |
| institution | Directory Open Access Journal |
| issn | 2196-5226 |
| language | deu |
| last_indexed | 2024-12-11T08:39:08Z |
| publishDate | 2014-08-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj.art-4d42fc33b0c9499e8ac9541f5ac22f7f2022-12-22T01:14:17ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262014-08-0192Doc1410.3205/dgkh000234In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumanniiPercin, Duygu0Akyol, Selcan1Kalin, Gamze2Department of Microbiology, Faculty of Medicine, Erciyes University, Kayseri, TurkeyDepartment of Microbiology, Istanbul University Cerrahpasa Medical Faculty, Istanbul, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Yozgat State Hospital, Yozgat, Turkey[english] Aim: The in vitro activity of colistin in combination with sulbactam, netilmicin, and vancomycin against colistin-resistant strains was investigated. Furthermore, the clonal relationship of the strains was analyzed. Methods: Clonal relationship was investigated using rep-PCR. To screen for synergysm, the fractional inhibitory concentration index (FICI) was calculated using checkerboard assay. The killing kinetics of the combination of colistin with vancomycin was assessed using time-kill assay. Results: Three different clones were found among 10 clinical isolates of colistin-resistant strains. Thereof, 8 strains were susceptible to netilmicin. Synergistic interaction was detected in 1 strain with the combination of colistin-netilmicin, in 5 strains with colistin-sulbactam, and in 9 strains with colistin-vancomycin. None of combinations had antagonistic activity. Colistin-vancomycin combination resulted in rapid bactericidal activity. Conclusion: These results show a distinct in vitro synergism between colistin and vancomycin, which might be useful to treat infection with multiple-resistant strains, prevent emergence of resistant strains, and to lower doses for both antibiotics to be used.http://www.egms.de/static/en/journals/dgkh/2014-9/dgkh000234.shtmlAcinetobacter baumanniicolistin resistancefractional inhibitory concentration index (FICI)time-kill assaysynergismvancomycinsulbactamnetilmicin |
| spellingShingle | Percin, Duygu Akyol, Selcan Kalin, Gamze In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii GMS Hygiene and Infection Control Acinetobacter baumannii colistin resistance fractional inhibitory concentration index (FICI) time-kill assay synergism vancomycin sulbactam netilmicin |
| title | In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii |
| title_full | In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii |
| title_fullStr | In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii |
| title_full_unstemmed | In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii |
| title_short | In vitro synergism of combinations of colistin with selected antibiotics against colistin-resistant Acinetobacter baumannii |
| title_sort | in vitro synergism of combinations of colistin with selected antibiotics against colistin resistant acinetobacter baumannii |
| topic | Acinetobacter baumannii colistin resistance fractional inhibitory concentration index (FICI) time-kill assay synergism vancomycin sulbactam netilmicin |
| url | http://www.egms.de/static/en/journals/dgkh/2014-9/dgkh000234.shtml |
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