Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria
Background and Objectives: Hadjr El Melh of Djelfa is an example of hypersaline ecosystems, which can harbor a wide variety of microorganisms under hostile physicochemical conditions. Given the importance of the study of halophilic microorganisms present there in terms of fundamental and applied mi...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-08-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3519 |
| _version_ | 1811283736040308736 |
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| author | Amira Beddal Saad Boutaiba Affaf Laassami Fella Hamaidi Madalin Enache |
| author_facet | Amira Beddal Saad Boutaiba Affaf Laassami Fella Hamaidi Madalin Enache |
| author_sort | Amira Beddal |
| collection | DOAJ |
| description |
Background and Objectives: Hadjr El Melh of Djelfa is an example of hypersaline ecosystems, which can harbor a wide variety of microorganisms under hostile physicochemical conditions. Given the importance of the study of halophilic microorganisms present there in terms of fundamental and applied microbiology, the purpose of this study was to characterize some halophilic archaea isolated from the brines of this environment.
Materials and Methods: Eight water samples were chosen randomly and collected for physicochemical and microbiological analyses. Isolation of halophilic archaea was carried out by membrane filter technique. Ten strains were identified by polyphasic approach and tested for enzymes production.
Results: Water samples of Djelfa’s rock salt were slightly acidic to neutral in pH (6.55-7.36) with salinity ranging from 258.68 g/l to 493.91 g/l. Phenotypic, biochemical, taxonomic and phylogenetic characteristics indicated that all strains were classified within the family of Halobacteiaceae. Based on the comparison of DNA sequences encoded 16S rRNA, it was determined that seven strains were affiliated to the genus Haloarcula, two strains were related to the genus Halobacterium and one strain within the genus Haloferax. Production of different enzymes such as protease, amylase, esterase, lipase, lecithinase, gelatinase and cellulase on solid medium indicated that one strain (S2-2) produced amylase, esterase, lecithinase and protease. However, no strains showed cellulolytic or lipolytic activity. Gelatinase was found in all tested strains.
Conclusion: This report constitutes the first preliminary study of culturable halophilic archaea recovered from the brines of Djelfa’s rock salt with a promising enzymatic potential in various fields of biotechnology.
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| first_indexed | 2024-04-13T02:16:49Z |
| format | Article |
| id | doaj.art-4d45a0f921894416b57c749556c79fc8 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-13T02:16:49Z |
| publishDate | 2022-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-4d45a0f921894416b57c749556c79fc82022-12-22T03:07:07ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-08-0114410.18502/ijm.v14i4.10240Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, AlgeriaAmira Beddal0Saad Boutaiba1Affaf Laassami2Fella Hamaidi3Madalin Enache4Department of Natural and Life Science, Faculty of Science and Technology, University of Tissemsilt, Bougara, Algeria; Department of Biology, Faculty of Natural and Life Science, University of Blida 1, Ouled Yaïch, Algeria; Department of Biology, Faculty of Natural and Life Science, University of Djelfa, Djelfa, AlgeriaDepartment of Biology, Faculty of Natural and Life Science, University of Djelfa, Djelfa, AlgeriaDepartment of Natural and Life Science, Ecole Normale Supérieure, Kouba, AlgiersDepartment of Biology, Faculty of Natural and Life Science, University of Blida 1, Ouled Yaïch, AlgeriaDepartment of Microbiology, Institute of Biology-Bucharest, Romanian Academy, Bucharest, Romania Background and Objectives: Hadjr El Melh of Djelfa is an example of hypersaline ecosystems, which can harbor a wide variety of microorganisms under hostile physicochemical conditions. Given the importance of the study of halophilic microorganisms present there in terms of fundamental and applied microbiology, the purpose of this study was to characterize some halophilic archaea isolated from the brines of this environment. Materials and Methods: Eight water samples were chosen randomly and collected for physicochemical and microbiological analyses. Isolation of halophilic archaea was carried out by membrane filter technique. Ten strains were identified by polyphasic approach and tested for enzymes production. Results: Water samples of Djelfa’s rock salt were slightly acidic to neutral in pH (6.55-7.36) with salinity ranging from 258.68 g/l to 493.91 g/l. Phenotypic, biochemical, taxonomic and phylogenetic characteristics indicated that all strains were classified within the family of Halobacteiaceae. Based on the comparison of DNA sequences encoded 16S rRNA, it was determined that seven strains were affiliated to the genus Haloarcula, two strains were related to the genus Halobacterium and one strain within the genus Haloferax. Production of different enzymes such as protease, amylase, esterase, lipase, lecithinase, gelatinase and cellulase on solid medium indicated that one strain (S2-2) produced amylase, esterase, lecithinase and protease. However, no strains showed cellulolytic or lipolytic activity. Gelatinase was found in all tested strains. Conclusion: This report constitutes the first preliminary study of culturable halophilic archaea recovered from the brines of Djelfa’s rock salt with a promising enzymatic potential in various fields of biotechnology. https://ijm.tums.ac.ir/index.php/ijm/article/view/3519Halobacteriaceae;Isolation and purification;Salinity;Enzyme;Archaea;Phylogeny; |
| spellingShingle | Amira Beddal Saad Boutaiba Affaf Laassami Fella Hamaidi Madalin Enache Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria Iranian Journal of Microbiology Halobacteriaceae; Isolation and purification; Salinity; Enzyme; Archaea; Phylogeny; |
| title | Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria |
| title_full | Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria |
| title_fullStr | Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria |
| title_full_unstemmed | Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria |
| title_short | Characterization by polyphasic approach of some indigenous halophilic archaea of Djelfa’s rock salt “Hadjr el Meelh”, Algeria |
| title_sort | characterization by polyphasic approach of some indigenous halophilic archaea of djelfa s rock salt hadjr el meelh algeria |
| topic | Halobacteriaceae; Isolation and purification; Salinity; Enzyme; Archaea; Phylogeny; |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3519 |
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