High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart
Mitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energet...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423623/?tool=EBI |
| _version_ | 1811302553919422464 |
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| author | Ana Karen Meza-Buendia Omar Emiliano Aparicio-Trejo Fernando Díaz Claudia Caamal-Monsreal José Pedraza-Chaverri Carolina Álvarez-Delgado Kurt Paschke Carlos Rosas |
| author_facet | Ana Karen Meza-Buendia Omar Emiliano Aparicio-Trejo Fernando Díaz Claudia Caamal-Monsreal José Pedraza-Chaverri Carolina Álvarez-Delgado Kurt Paschke Carlos Rosas |
| author_sort | Ana Karen Meza-Buendia |
| collection | DOAJ |
| description | Mitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energetic demands of animals in high temperatures. In a recent study made in Octopus maya was proposed the hypothesis postulating that high temperatures could restrain female reproduction due to the limited capacity of the animals’ heart to sustain oxygen flow to the body, affecting in this manner energy production in the rest of the organs, including the ovarium Meza-Buendia AK et al. (2021). Unfortunately, until now, no reports have shown temperature effects and other environmental variables on cephalopod mitochondria activity because of the lack of a method to evaluate mitochondrial respiratory parameters in those species’ groups. In this sense and for the first time, this study developed a method to obtain mitochondrial respirometry data of adult Octopus maya’s heart. This protocol illustrates a step-by-step procedure to get high yield and functional mitochondria of cephalopod heart and procedure for determining the corresponding respiratory parameters. The procedure described in this paper takes approximately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption. |
| first_indexed | 2024-04-13T07:30:10Z |
| format | Article |
| id | doaj.art-4d45bd3e21054f16889be15af0cb72f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T07:30:10Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4d45bd3e21054f16889be15af0cb72f72022-12-22T02:56:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01178High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heartAna Karen Meza-BuendiaOmar Emiliano Aparicio-TrejoFernando DíazClaudia Caamal-MonsrealJosé Pedraza-ChaverriCarolina Álvarez-DelgadoKurt PaschkeCarlos RosasMitochondrial respirometry is key to understand how environmental factors model energetic cellular process. In the case of ectotherms, thermal tolerance has been hypothesized to be intimately linked with mitochondria capability to produce enough adenosine triphosphate (ATP) to respond to the energetic demands of animals in high temperatures. In a recent study made in Octopus maya was proposed the hypothesis postulating that high temperatures could restrain female reproduction due to the limited capacity of the animals’ heart to sustain oxygen flow to the body, affecting in this manner energy production in the rest of the organs, including the ovarium Meza-Buendia AK et al. (2021). Unfortunately, until now, no reports have shown temperature effects and other environmental variables on cephalopod mitochondria activity because of the lack of a method to evaluate mitochondrial respiratory parameters in those species’ groups. In this sense and for the first time, this study developed a method to obtain mitochondrial respirometry data of adult Octopus maya’s heart. This protocol illustrates a step-by-step procedure to get high yield and functional mitochondria of cephalopod heart and procedure for determining the corresponding respiratory parameters. The procedure described in this paper takes approximately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423623/?tool=EBI |
| spellingShingle | Ana Karen Meza-Buendia Omar Emiliano Aparicio-Trejo Fernando Díaz Claudia Caamal-Monsreal José Pedraza-Chaverri Carolina Álvarez-Delgado Kurt Paschke Carlos Rosas High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart PLoS ONE |
| title | High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart |
| title_full | High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart |
| title_fullStr | High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart |
| title_full_unstemmed | High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart |
| title_short | High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart |
| title_sort | high resolution respirometry of isolated mitochondria from adult octopus maya class cephalopoda systemic heart |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423623/?tool=EBI |
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