Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer
Components of tumor microenvironment, including tumor and/or stromal cells-derived factors, exert a critical role in breast cancer (BC) progression. Here we evaluated the possible role of neuroglobin (NGB), a monomeric globin that acts as a compensatory protein against oxidative and apoptotic proces...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/9/2451 |
| _version_ | 1797555179243962368 |
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| author | Marco Fiocchetti Virginia Solar Fernandez Marco Segatto Stefano Leone Paolo Cercola Annalisa Massari Francesco Cavaliere Maria Marino |
| author_facet | Marco Fiocchetti Virginia Solar Fernandez Marco Segatto Stefano Leone Paolo Cercola Annalisa Massari Francesco Cavaliere Maria Marino |
| author_sort | Marco Fiocchetti |
| collection | DOAJ |
| description | Components of tumor microenvironment, including tumor and/or stromal cells-derived factors, exert a critical role in breast cancer (BC) progression. Here we evaluated the possible role of neuroglobin (NGB), a monomeric globin that acts as a compensatory protein against oxidative and apoptotic processes, as part of BC microenvironment. The extracellular NGB levels were evaluated by immunofluorescence of BC tissue sections and by Western blot of the culture media of BC cell lines. Moreover, reactive oxygen species (ROS) generation, cell apoptosis, and cell migration were evaluated in different BC cells and non-tumorigenic epithelial mammary cells treated with BC cells (i.e., Michigan Cancer Foundation-7, MCF-7) conditioned culture media and extracellular NGB. Results demonstrate that NGB is a component of BC microenvironment. NGB is released in tumor microenvironment by BC cells only under oxidative stress conditions where it can act as autocrine/paracrine factor able to communicate cell resilience against oxidative stress and chemotherapeutic treatment. |
| first_indexed | 2024-03-10T16:43:46Z |
| format | Article |
| id | doaj.art-4d4605281cd8480eaed063181548d515 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T16:43:46Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-4d4605281cd8480eaed063181548d5152023-11-20T11:48:25ZengMDPI AGCancers2072-66942020-08-01129245110.3390/cancers12092451Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast CancerMarco Fiocchetti0Virginia Solar Fernandez1Marco Segatto2Stefano Leone3Paolo Cercola4Annalisa Massari5Francesco Cavaliere6Maria Marino7Department of Science, University Roma Tre, Viale Guglielmo Marconi 446, I-00146 Roma, ItalyDepartment of Science, University Roma Tre, Viale Guglielmo Marconi 446, I-00146 Roma, ItalyDepartment of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, 86090 Pesche (IS), ItalyDepartment of Science, University Roma Tre, Viale Guglielmo Marconi 446, I-00146 Roma, ItalyDivision of Senology, Belcolle Hospital, Str. Sammartinese, 01100 Viterbo, ItalyDivision of Senology, Belcolle Hospital, Str. Sammartinese, 01100 Viterbo, ItalyDivision of Senology, Belcolle Hospital, Str. Sammartinese, 01100 Viterbo, ItalyDepartment of Science, University Roma Tre, Viale Guglielmo Marconi 446, I-00146 Roma, ItalyComponents of tumor microenvironment, including tumor and/or stromal cells-derived factors, exert a critical role in breast cancer (BC) progression. Here we evaluated the possible role of neuroglobin (NGB), a monomeric globin that acts as a compensatory protein against oxidative and apoptotic processes, as part of BC microenvironment. The extracellular NGB levels were evaluated by immunofluorescence of BC tissue sections and by Western blot of the culture media of BC cell lines. Moreover, reactive oxygen species (ROS) generation, cell apoptosis, and cell migration were evaluated in different BC cells and non-tumorigenic epithelial mammary cells treated with BC cells (i.e., Michigan Cancer Foundation-7, MCF-7) conditioned culture media and extracellular NGB. Results demonstrate that NGB is a component of BC microenvironment. NGB is released in tumor microenvironment by BC cells only under oxidative stress conditions where it can act as autocrine/paracrine factor able to communicate cell resilience against oxidative stress and chemotherapeutic treatment.https://www.mdpi.com/2072-6694/12/9/245117β-Estradiolapoptosisbreast cancerdocetaxelneuroglobinoxidative stress |
| spellingShingle | Marco Fiocchetti Virginia Solar Fernandez Marco Segatto Stefano Leone Paolo Cercola Annalisa Massari Francesco Cavaliere Maria Marino Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer Cancers 17β-Estradiol apoptosis breast cancer docetaxel neuroglobin oxidative stress |
| title | Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer |
| title_full | Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer |
| title_fullStr | Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer |
| title_full_unstemmed | Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer |
| title_short | Extracellular Neuroglobin as a Stress-Induced Factor Activating Pre-Adaptation Mechanisms against Oxidative Stress and Chemotherapy-Induced Cell Death in Breast Cancer |
| title_sort | extracellular neuroglobin as a stress induced factor activating pre adaptation mechanisms against oxidative stress and chemotherapy induced cell death in breast cancer |
| topic | 17β-Estradiol apoptosis breast cancer docetaxel neuroglobin oxidative stress |
| url | https://www.mdpi.com/2072-6694/12/9/2451 |
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