Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant
Buckwheat (<i>Fagopyrum esculentum</i>, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompa...
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MDPI AG
2019-11-01
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author | Yue Fei Lan-Xiang Wang Zheng-Wu Fang Zhi-Xiong Liu |
author_facet | Yue Fei Lan-Xiang Wang Zheng-Wu Fang Zhi-Xiong Liu |
author_sort | Yue Fei |
collection | DOAJ |
description | Buckwheat (<i>Fagopyrum esculentum</i>, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of <i>F. esculentum</i>. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL<sup>−1</sup> 2,4-D and 1.5 mgL<sup>−1</sup> 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL<sup>−1</sup> 6-BA and 0.5 mgL<sup>−1</sup> thidiazuron (TDZ)two to three times subculture at 40−60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL<sup>−1</sup> 6-BA and 0.5 mgL<sup>−1</sup> TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of <i>F. esculentum</i>. |
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spelling | doaj.art-4d46247de5714312ad9fd696bebf77742022-12-21T22:24:40ZengMDPI AGAgronomy2073-43952019-11-0191176810.3390/agronomy9110768agronomy9110768Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> MutantYue Fei0Lan-Xiang Wang1Zheng-Wu Fang2Zhi-Xiong Liu3College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, ChinaInstitute of Crop Genetics and Breeding, Yangtze University, Jingzhou 434025, Hubei, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, ChinaBuckwheat (<i>Fagopyrum esculentum</i>, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of <i>F. esculentum</i>. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL<sup>−1</sup> 2,4-D and 1.5 mgL<sup>−1</sup> 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL<sup>−1</sup> 6-BA and 0.5 mgL<sup>−1</sup> thidiazuron (TDZ)two to three times subculture at 40−60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL<sup>−1</sup> 6-BA and 0.5 mgL<sup>−1</sup> TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of <i>F. esculentum</i>.https://www.mdpi.com/2073-4395/9/11/768callus induction<i>fagopyrum esculentum</i>hypocotyl explantsplant regenerationsomatic embryogenesis |
spellingShingle | Yue Fei Lan-Xiang Wang Zheng-Wu Fang Zhi-Xiong Liu Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant Agronomy callus induction <i>fagopyrum esculentum</i> hypocotyl explants plant regeneration somatic embryogenesis |
title | Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant |
title_full | Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant |
title_fullStr | Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant |
title_full_unstemmed | Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant |
title_short | Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of <i>Fagopyrum esculentum</i> Moench <i>lpls</i> Mutant |
title_sort | somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of i fagopyrum esculentum i moench i lpls i mutant |
topic | callus induction <i>fagopyrum esculentum</i> hypocotyl explants plant regeneration somatic embryogenesis |
url | https://www.mdpi.com/2073-4395/9/11/768 |
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