Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr.
Fungi-orchid relationships have been studied mainly in terrestrial orchids. The present work deals with diversity and biological role of the fungi associated to the epiphytic orchid Laelia speciosa through molecular evidence. ITS sequences confi rmed fungal presence in roots, capsules, seeds and in...
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| Format: | Article |
| Language: | English |
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Sociedad Botánica de México, A. C.
2013-12-01
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| Series: | Botanical Sciences |
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| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/429 |
| _version_ | 1798017771772051456 |
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| author | Irene Ávila-Díaz Roberto Garibay-Orijel Rosa Elia Magaña-Lemus Ken Oyama |
| author_facet | Irene Ávila-Díaz Roberto Garibay-Orijel Rosa Elia Magaña-Lemus Ken Oyama |
| author_sort | Irene Ávila-Díaz |
| collection | DOAJ |
| description |
Fungi-orchid relationships have been studied mainly in terrestrial orchids. The present work deals with diversity and biological role of the fungi associated to the epiphytic orchid Laelia speciosa through molecular evidence. ITS sequences confi rmed fungal presence in roots, capsules, seeds and in vitro seedlings. None of the fungi found formed characteristic pelotons, so we do not assume they are mycorrhizal. We identifi ed 18 taxa of fungi belonging to eight known genera (Alternaria, Curvularia, Cylindrocarpon, Fusarium, Myrmecridium, Neonectria, Penicillium, and Tetracladium) and unknown species of Atractiellales, Helotiales, Hypocreales, Lasiosphaeriaceae, Nectriaceae, Sordariomycetes, and Tricholomataceae. From these, we infer the biology of nine parasites, four saprobes, and two endophytes. One of the fungi, a Helotiales species, was able to colonize all orchid tissues, including seeds and in vitro seedlings, so, even if all precautions were taken, “axenic culture” of L. speciosa is a diffi cult task. There is a considerable diversity of fungi within L. speciosa organs. Endophytic fungi interact with the whole plant of L. speciosa along its life cycle.
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| first_indexed | 2024-04-11T16:13:08Z |
| format | Article |
| id | doaj.art-4d4b3fe87dc64fe4bc1406d993580ac2 |
| institution | Directory Open Access Journal |
| issn | 2007-4298 2007-4476 |
| language | English |
| last_indexed | 2024-04-11T16:13:08Z |
| publishDate | 2013-12-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj.art-4d4b3fe87dc64fe4bc1406d993580ac22022-12-22T04:14:37ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762013-12-0191410.17129/botsci.429Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr.Irene Ávila-Díaz0Roberto Garibay-Orijel1Rosa Elia Magaña-Lemus2Ken Oyama3Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, Me2 Instituto de Biología, Universidad Nacional Autónoma de México, México, D.F., MexicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MexicoCentro de Investigaciones en Ecosistemas y Escuela Nacional de Estudios Superiores Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, Mexi Fungi-orchid relationships have been studied mainly in terrestrial orchids. The present work deals with diversity and biological role of the fungi associated to the epiphytic orchid Laelia speciosa through molecular evidence. ITS sequences confi rmed fungal presence in roots, capsules, seeds and in vitro seedlings. None of the fungi found formed characteristic pelotons, so we do not assume they are mycorrhizal. We identifi ed 18 taxa of fungi belonging to eight known genera (Alternaria, Curvularia, Cylindrocarpon, Fusarium, Myrmecridium, Neonectria, Penicillium, and Tetracladium) and unknown species of Atractiellales, Helotiales, Hypocreales, Lasiosphaeriaceae, Nectriaceae, Sordariomycetes, and Tricholomataceae. From these, we infer the biology of nine parasites, four saprobes, and two endophytes. One of the fungi, a Helotiales species, was able to colonize all orchid tissues, including seeds and in vitro seedlings, so, even if all precautions were taken, “axenic culture” of L. speciosa is a diffi cult task. There is a considerable diversity of fungi within L. speciosa organs. Endophytic fungi interact with the whole plant of L. speciosa along its life cycle. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/429fungiITS sequencesin vitro culture, |
| spellingShingle | Irene Ávila-Díaz Roberto Garibay-Orijel Rosa Elia Magaña-Lemus Ken Oyama Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr. Botanical Sciences fungi ITS sequences in vitro culture, |
| title | Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr. |
| title_full | Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr. |
| title_fullStr | Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr. |
| title_full_unstemmed | Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr. |
| title_short | Molecular evidence reveals fungi associated within the epiphytic orchid <em>Laelia speciosa</em> (HBK) Schltr. |
| title_sort | molecular evidence reveals fungi associated within the epiphytic orchid em laelia speciosa em hbk schltr |
| topic | fungi ITS sequences in vitro culture, |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/429 |
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