Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition
Characterizing the molecular mechanism through which different carbon sources affect the denitrification process would provide a basis for the proper selection of carbon sources, thus avoiding excessive carbon source dosing and secondary pollution while also improving denitrification efficiency. Her...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412022005256 |
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| author | Qi Wei Jinsen Zhang Fangzhou Luo Dinghuan Shi Yuchen Liu Shuai Liu Qian Zhang Wenzhuo Sun Junli Yuan Haitao Fan Hongchen Wang Lu Qi Guohua Liu |
| author_facet | Qi Wei Jinsen Zhang Fangzhou Luo Dinghuan Shi Yuchen Liu Shuai Liu Qian Zhang Wenzhuo Sun Junli Yuan Haitao Fan Hongchen Wang Lu Qi Guohua Liu |
| author_sort | Qi Wei |
| collection | DOAJ |
| description | Characterizing the molecular mechanism through which different carbon sources affect the denitrification process would provide a basis for the proper selection of carbon sources, thus avoiding excessive carbon source dosing and secondary pollution while also improving denitrification efficiency. Here, we selected Thauera linaloolentis as a model organism of denitrification, whose genomic information was elucidated by draft genome sequencing and KEGG annotations, to investigate the growth kinetics, denitrification performances and characteristics of metabolic pathways under diverse carbon source conditions. We reconstructed a metabolic network of Thauera linaloolentis based on genomic analysis to help develop a systematic method of researching electron pathways. Our findings indicated that carbon sources with simple metabolic pathways (e.g., ethanol and sodium acetate) promoted the reproduction of Thauera linaloolentis, and its maximum growth density reached OD600 = 0.36 and maximum specific growth rate reached 0.145 h−1. These carbon sources also accelerated the denitrification process without the accumulation of intermediates. Nitrate could be reduced completely under any carbon source condition; but in the “glucose group”, the maximum accumulation of nitrite was 117.00 mg/L (1.51 times more than that in the “ethanol group”, which was 77.41 mg/L), the maximum accumulation of nitric oxide was 363.02 μg/L (7.35 times more than that in the “ethanol group”, which was 49.40 μg/L), and the maximum accumulation of nitrous oxide was 22.58 mg/L (26.56 times more than that in the “ethanol group”, which was 0.85 mg/L). Molecular biological analyses demonstrated that diverse types of carbon sources directly induced different carbon metabolic activities, resulting in variations in electron generation efficiency. Furthermore, the activities of the electron transport system were positively correlated with different carbon metabolic activities. Finally, these differences were reflected in the phenomenon of electronic competition between denitrifying reductases. Thus we concluded that this was the main molecular mechanism through which the carbon source type affected the denitrification process. In brief, carbon sources with simple metabolic pathways induced higher efficiency of electron generation, transfer, and competition, which promoted rapid proliferation and complete denitrification; otherwise Thauera linaloolentis would grow slowly and intermediate products would accumulate seriously. Our study established a method to evaluate and optimize carbon source utilization efficiency based on confirmed molecular mechanisms. |
| first_indexed | 2024-04-12T03:55:02Z |
| format | Article |
| id | doaj.art-4d4c528d445f4c64ae72f1c56b2cc6ba |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-04-12T03:55:02Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-4d4c528d445f4c64ae72f1c56b2cc6ba2022-12-22T03:48:52ZengElsevierEnvironment International0160-41202022-12-01170107598Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competitionQi Wei0Jinsen Zhang1Fangzhou Luo2Dinghuan Shi3Yuchen Liu4Shuai Liu5Qian Zhang6Wenzhuo Sun7Junli Yuan8Haitao Fan9Hongchen Wang10Lu Qi11Guohua Liu12Low-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaCorresponding authors.; Low-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaCorresponding authors.; Low-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaLow-carbon Water Environmental Technology Center, School of Environment & Natural Resource, Renmin University of China, 59 Zhongguancun Street, Beijing 100872, PR ChinaCharacterizing the molecular mechanism through which different carbon sources affect the denitrification process would provide a basis for the proper selection of carbon sources, thus avoiding excessive carbon source dosing and secondary pollution while also improving denitrification efficiency. Here, we selected Thauera linaloolentis as a model organism of denitrification, whose genomic information was elucidated by draft genome sequencing and KEGG annotations, to investigate the growth kinetics, denitrification performances and characteristics of metabolic pathways under diverse carbon source conditions. We reconstructed a metabolic network of Thauera linaloolentis based on genomic analysis to help develop a systematic method of researching electron pathways. Our findings indicated that carbon sources with simple metabolic pathways (e.g., ethanol and sodium acetate) promoted the reproduction of Thauera linaloolentis, and its maximum growth density reached OD600 = 0.36 and maximum specific growth rate reached 0.145 h−1. These carbon sources also accelerated the denitrification process without the accumulation of intermediates. Nitrate could be reduced completely under any carbon source condition; but in the “glucose group”, the maximum accumulation of nitrite was 117.00 mg/L (1.51 times more than that in the “ethanol group”, which was 77.41 mg/L), the maximum accumulation of nitric oxide was 363.02 μg/L (7.35 times more than that in the “ethanol group”, which was 49.40 μg/L), and the maximum accumulation of nitrous oxide was 22.58 mg/L (26.56 times more than that in the “ethanol group”, which was 0.85 mg/L). Molecular biological analyses demonstrated that diverse types of carbon sources directly induced different carbon metabolic activities, resulting in variations in electron generation efficiency. Furthermore, the activities of the electron transport system were positively correlated with different carbon metabolic activities. Finally, these differences were reflected in the phenomenon of electronic competition between denitrifying reductases. Thus we concluded that this was the main molecular mechanism through which the carbon source type affected the denitrification process. In brief, carbon sources with simple metabolic pathways induced higher efficiency of electron generation, transfer, and competition, which promoted rapid proliferation and complete denitrification; otherwise Thauera linaloolentis would grow slowly and intermediate products would accumulate seriously. Our study established a method to evaluate and optimize carbon source utilization efficiency based on confirmed molecular mechanisms.http://www.sciencedirect.com/science/article/pii/S0160412022005256Carbon sourceDenitrificationThauera linaloolentisMolecular mechanismElectron transport system |
| spellingShingle | Qi Wei Jinsen Zhang Fangzhou Luo Dinghuan Shi Yuchen Liu Shuai Liu Qian Zhang Wenzhuo Sun Junli Yuan Haitao Fan Hongchen Wang Lu Qi Guohua Liu Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition Environment International Carbon source Denitrification Thauera linaloolentis Molecular mechanism Electron transport system |
| title | Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition |
| title_full | Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition |
| title_fullStr | Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition |
| title_full_unstemmed | Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition |
| title_short | Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition |
| title_sort | molecular mechanisms through which different carbon sources affect denitrification by thauera linaloolentis electron generation transfer and competition |
| topic | Carbon source Denitrification Thauera linaloolentis Molecular mechanism Electron transport system |
| url | http://www.sciencedirect.com/science/article/pii/S0160412022005256 |
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