Multiplex base editing to convert TAG into TAA codons in the human genome
Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here the authors extend this to human cells using base editing to convert TAG to TAA for 33 essential genes via a single transfection followed by examining base-editing genome-wid...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31927-8 |
| _version_ | 1811342829203488768 |
|---|---|
| author | Yuting Chen Eriona Hysolli Anlu Chen Stephen Casper Songlei Liu Kevin Yang Chenli Liu George Church |
| author_facet | Yuting Chen Eriona Hysolli Anlu Chen Stephen Casper Songlei Liu Kevin Yang Chenli Liu George Church |
| author_sort | Yuting Chen |
| collection | DOAJ |
| description | Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here the authors extend this to human cells using base editing to convert TAG to TAA for 33 essential genes via a single transfection followed by examining base-editing genome-wide. |
| first_indexed | 2024-04-13T19:17:50Z |
| format | Article |
| id | doaj.art-4d4f5949126f4732aa4cc9b34be9ee16 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T19:17:50Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4d4f5949126f4732aa4cc9b34be9ee162022-12-22T02:33:37ZengNature PortfolioNature Communications2041-17232022-08-0113111310.1038/s41467-022-31927-8Multiplex base editing to convert TAG into TAA codons in the human genomeYuting Chen0Eriona Hysolli1Anlu Chen2Stephen Casper3Songlei Liu4Kevin Yang5Chenli Liu6George Church7Department of Genetics, Harvard Medical SchoolDepartment of Genetics, Harvard Medical SchoolDivision of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical SchoolDepartment of Genetics, Harvard Medical SchoolDepartment of Genetics, Harvard Medical SchoolDepartment of Genetics, Harvard Medical SchoolCAS Key Laboratory of Quantitative Engineering Biology, Center for Genome Engineering and Therapy, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of SciencesDepartment of Genetics, Harvard Medical SchoolWhole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here the authors extend this to human cells using base editing to convert TAG to TAA for 33 essential genes via a single transfection followed by examining base-editing genome-wide.https://doi.org/10.1038/s41467-022-31927-8 |
| spellingShingle | Yuting Chen Eriona Hysolli Anlu Chen Stephen Casper Songlei Liu Kevin Yang Chenli Liu George Church Multiplex base editing to convert TAG into TAA codons in the human genome Nature Communications |
| title | Multiplex base editing to convert TAG into TAA codons in the human genome |
| title_full | Multiplex base editing to convert TAG into TAA codons in the human genome |
| title_fullStr | Multiplex base editing to convert TAG into TAA codons in the human genome |
| title_full_unstemmed | Multiplex base editing to convert TAG into TAA codons in the human genome |
| title_short | Multiplex base editing to convert TAG into TAA codons in the human genome |
| title_sort | multiplex base editing to convert tag into taa codons in the human genome |
| url | https://doi.org/10.1038/s41467-022-31927-8 |
| work_keys_str_mv | AT yutingchen multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT erionahysolli multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT anluchen multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT stephencasper multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT songleiliu multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT kevinyang multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT chenliliu multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome AT georgechurch multiplexbaseeditingtoconverttagintotaacodonsinthehumangenome |