Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions
Background and Objectives: Different types of antibiotics have been indicated to enhance the secretion of OMVs from Pseudomonas aeruginosa. We aimed to investigate the effect of meropenem and amikacin antibiotics on inducing the secretion of OMVs and immunologic features in P. aeruginosa. Materials...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3299 |
| _version_ | 1798027991345790976 |
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| author | Mehdi Hadadi-Fishani Shahin Najar-Peerayeh Seyed Davar Siadat Mohammad Sekhavati Ashraf Mohabati Mobarez |
| author_facet | Mehdi Hadadi-Fishani Shahin Najar-Peerayeh Seyed Davar Siadat Mohammad Sekhavati Ashraf Mohabati Mobarez |
| author_sort | Mehdi Hadadi-Fishani |
| collection | DOAJ |
| description | Background and Objectives: Different types of antibiotics have been indicated to enhance the secretion of OMVs from Pseudomonas aeruginosa. We aimed to investigate the effect of meropenem and amikacin antibiotics on inducing the secretion of OMVs and immunologic features in P. aeruginosa.
Materials and Methods: The OMVs were prepared from P. aeruginosa under hypervesiculation condition (treatment with amikacin and meropenem), and extraction was carried out by the sequential ultracentrifugation. Physicochemical features of extracted OMVs were evaluated by electron microscopy and SDS-PAGE. To quantify antibody synthesis and function after immunization with OMV, we used ELISA, serum bactericidal activity, and opsonophagocytosis. Production of cytokines from splenocytes of immunized mice was measured with ELISA.
Results: Specific-antibody IgG production, particularly IgG1 subclass, increased in mice primed with hypervesiculation-derived OMVs compared to normal condition-derived OMVs. Serum bactericidal activity and opsonophagocytosis of secreted antibody was enhanced in mice primed with hypervesiculation-derived OMVs. Investigation of cytokine production showed the upregulation of IL-8, IL-12, IL-17, and TNF-α and downregulation of IL-10.
Conclusion: Based on our findings, OMVs production can be increased by treating P. aeruginosa with amikacin and meropenem antibiotics. Moreover, hypervesiculation-derived OMV scan possibly activate the humoral and cellular immune response more than normal OMVs. |
| first_indexed | 2024-04-11T19:00:37Z |
| format | Article |
| id | doaj.art-4d510c9088e04630a2b9cdd76299d4d6 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-11T19:00:37Z |
| publishDate | 2021-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-4d510c9088e04630a2b9cdd76299d4d62022-12-22T04:08:03ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472021-12-01136Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditionsMehdi Hadadi-Fishani0Shahin Najar-Peerayeh1Seyed Davar Siadat2Mohammad Sekhavati3Ashraf Mohabati Mobarez4Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute, Tehran, IranDepartment of Aerobic Bacterial Research and Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranBackground and Objectives: Different types of antibiotics have been indicated to enhance the secretion of OMVs from Pseudomonas aeruginosa. We aimed to investigate the effect of meropenem and amikacin antibiotics on inducing the secretion of OMVs and immunologic features in P. aeruginosa. Materials and Methods: The OMVs were prepared from P. aeruginosa under hypervesiculation condition (treatment with amikacin and meropenem), and extraction was carried out by the sequential ultracentrifugation. Physicochemical features of extracted OMVs were evaluated by electron microscopy and SDS-PAGE. To quantify antibody synthesis and function after immunization with OMV, we used ELISA, serum bactericidal activity, and opsonophagocytosis. Production of cytokines from splenocytes of immunized mice was measured with ELISA. Results: Specific-antibody IgG production, particularly IgG1 subclass, increased in mice primed with hypervesiculation-derived OMVs compared to normal condition-derived OMVs. Serum bactericidal activity and opsonophagocytosis of secreted antibody was enhanced in mice primed with hypervesiculation-derived OMVs. Investigation of cytokine production showed the upregulation of IL-8, IL-12, IL-17, and TNF-α and downregulation of IL-10. Conclusion: Based on our findings, OMVs production can be increased by treating P. aeruginosa with amikacin and meropenem antibiotics. Moreover, hypervesiculation-derived OMV scan possibly activate the humoral and cellular immune response more than normal OMVs.https://ijm.tums.ac.ir/index.php/ijm/article/view/3299Outer membrane vesicle;Pseudomonas aeruginosa;Hypervesiculation;Immunization |
| spellingShingle | Mehdi Hadadi-Fishani Shahin Najar-Peerayeh Seyed Davar Siadat Mohammad Sekhavati Ashraf Mohabati Mobarez Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions Iranian Journal of Microbiology Outer membrane vesicle; Pseudomonas aeruginosa; Hypervesiculation; Immunization |
| title | Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions |
| title_full | Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions |
| title_fullStr | Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions |
| title_full_unstemmed | Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions |
| title_short | Isolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions |
| title_sort | isolation and immunogenicity of extracted outer membrane vesicles from pseudomonas aeruginosa under antibiotics treatment conditions |
| topic | Outer membrane vesicle; Pseudomonas aeruginosa; Hypervesiculation; Immunization |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3299 |
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