A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress
In this study, <i>Scenedesmus</i> sp. FSP3 was cultured using a two-stage culture strategy for CO<sub>2</sub> fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO<sub>2</sub> introduced to promote the rapi...
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/21/7497 |
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| author | Jiawei Li Xinqing Zhao Jo-Shu Chang Xiaoling Miao |
| author_facet | Jiawei Li Xinqing Zhao Jo-Shu Chang Xiaoling Miao |
| author_sort | Jiawei Li |
| collection | DOAJ |
| description | In this study, <i>Scenedesmus</i> sp. FSP3 was cultured using a two-stage culture strategy for CO<sub>2</sub> fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO<sub>2</sub> introduced to promote the rapid growth and CO<sub>2</sub> fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L<sup>−1</sup> and a light intensity of 160 μmol m<sup>−2</sup> s<sup>−1</sup> were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO<sub>2</sub> fixation were simultaneously achieved. The biomass productivity and carbon fixation rate of <i>Scenedesmus</i> sp. FSP3 reached 0.58 g L<sup>−1</sup> d<sup>−1</sup> and 1.09 g L<sup>−1</sup> d<sup>−1</sup>, with a lutein content and yield as high as 6.45 mg g<sup>−1</sup> and 2.30 mg L<sup>−1</sup> d<sup>−1</sup>, respectively. The results reveal a commercially feasible way to integrate microalgal lutein production with CO<sub>2</sub> fixation processes. |
| first_indexed | 2024-03-09T18:47:51Z |
| format | Article |
| id | doaj.art-4d5137dad58e411591f30f0902321bf3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:47:51Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4d5137dad58e411591f30f0902321bf32023-11-24T06:05:12ZengMDPI AGMolecules1420-30492022-11-012721749710.3390/molecules27217497A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt StressJiawei Li0Xinqing Zhao1Jo-Shu Chang2Xiaoling Miao3State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaDepartment of Chemical and Materials Engineering, Tunghai University, Taichung 407, TaiwanState Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, ChinaIn this study, <i>Scenedesmus</i> sp. FSP3 was cultured using a two-stage culture strategy for CO<sub>2</sub> fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO<sub>2</sub> introduced to promote the rapid growth and CO<sub>2</sub> fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L<sup>−1</sup> and a light intensity of 160 μmol m<sup>−2</sup> s<sup>−1</sup> were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO<sub>2</sub> fixation were simultaneously achieved. The biomass productivity and carbon fixation rate of <i>Scenedesmus</i> sp. FSP3 reached 0.58 g L<sup>−1</sup> d<sup>−1</sup> and 1.09 g L<sup>−1</sup> d<sup>−1</sup>, with a lutein content and yield as high as 6.45 mg g<sup>−1</sup> and 2.30 mg L<sup>−1</sup> d<sup>−1</sup>, respectively. The results reveal a commercially feasible way to integrate microalgal lutein production with CO<sub>2</sub> fixation processes.https://www.mdpi.com/1420-3049/27/21/7497lutein<i>Scenedesmus</i> sp. FSP3CO<sub>2</sub> fixationlight stresssalt stress |
| spellingShingle | Jiawei Li Xinqing Zhao Jo-Shu Chang Xiaoling Miao A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress Molecules lutein <i>Scenedesmus</i> sp. FSP3 CO<sub>2</sub> fixation light stress salt stress |
| title | A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress |
| title_full | A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress |
| title_fullStr | A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress |
| title_full_unstemmed | A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress |
| title_short | A Two-Stage Culture Strategy for <i>Scenedesmus</i> sp. FSP3 for CO<sub>2</sub> Fixation and the Simultaneous Production of Lutein under Light and Salt Stress |
| title_sort | two stage culture strategy for i scenedesmus i sp fsp3 for co sub 2 sub fixation and the simultaneous production of lutein under light and salt stress |
| topic | lutein <i>Scenedesmus</i> sp. FSP3 CO<sub>2</sub> fixation light stress salt stress |
| url | https://www.mdpi.com/1420-3049/27/21/7497 |
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