Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse
Homomeric α7 nicotinic acetylcholine receptors (nAChRs) are abundantly expressed in the central and peripheral nervous system (CNS and PNS, respectively), and spinal cord. In addition, expression and functional responses have been reported in non-neuronal tissue. In the nervous system, α7 nAChR subu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnana.2019.00076/full |
| _version_ | 1818195992516755456 |
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| author | Ron S. Broide Ursula H. Winzer-Serhan Yling Chen Frances M. Leslie |
| author_facet | Ron S. Broide Ursula H. Winzer-Serhan Yling Chen Frances M. Leslie |
| author_sort | Ron S. Broide |
| collection | DOAJ |
| description | Homomeric α7 nicotinic acetylcholine receptors (nAChRs) are abundantly expressed in the central and peripheral nervous system (CNS and PNS, respectively), and spinal cord. In addition, expression and functional responses have been reported in non-neuronal tissue. In the nervous system, α7 nAChR subunit expression appears early during embryonic development and is often transiently upregulated, but little is known about their prenatal expression outside of the nervous system. For understanding potential short-term and long-term effects of gestational nicotine exposure, it is important to know the temporal and spatial expression of α7 nAChRs throughout the body. To that end, we studied the expression of α7 nAChR subunit mRNA using highly sensitive isotopic in situ hybridization in embryonic and neonatal whole-body mouse sections starting at gestational day 13. The results revealed expression of α7 mRNA as early as embryonic day 13 in the PNS, including dorsal root ganglia, parasympathetic and sympathetic ganglia, with the strongest expression in the superior cervical ganglion, and low to moderate levels were detected in brain and spinal cord, respectively, which rapidly increased in intensity with embryonic age. In addition, robust α7 mRNA expression was detected in the adrenal medulla, and low to moderate expression in selected peripheral tissues during embryonic development, potentially related to cells derived from the neural crest. Little or no mRNA expression was detected in thymus or spleen, sites of immune cell maturation. The results suggest that prenatal nicotine exposure could potentially affect the nervous system with limited effects in non-neural tissues. |
| first_indexed | 2024-12-12T01:27:00Z |
| format | Article |
| id | doaj.art-4d5301a9986b49309362295e883bd939 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-12T01:27:00Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-4d5301a9986b49309362295e883bd9392022-12-22T00:43:04ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292019-08-011310.3389/fnana.2019.00076465985Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing MouseRon S. Broide0Ursula H. Winzer-Serhan1Yling Chen2Frances M. Leslie3Department of Pharmacology, University of California, Irvine, Irvine, CA, United StatesDepartment of Neuroscience and Experimental Therapeutics, Texas A&M University College of Medicine, Bryan, TX, United StatesDepartment of Pharmacology, University of California, Irvine, Irvine, CA, United StatesDepartment of Pharmacology, University of California, Irvine, Irvine, CA, United StatesHomomeric α7 nicotinic acetylcholine receptors (nAChRs) are abundantly expressed in the central and peripheral nervous system (CNS and PNS, respectively), and spinal cord. In addition, expression and functional responses have been reported in non-neuronal tissue. In the nervous system, α7 nAChR subunit expression appears early during embryonic development and is often transiently upregulated, but little is known about their prenatal expression outside of the nervous system. For understanding potential short-term and long-term effects of gestational nicotine exposure, it is important to know the temporal and spatial expression of α7 nAChRs throughout the body. To that end, we studied the expression of α7 nAChR subunit mRNA using highly sensitive isotopic in situ hybridization in embryonic and neonatal whole-body mouse sections starting at gestational day 13. The results revealed expression of α7 mRNA as early as embryonic day 13 in the PNS, including dorsal root ganglia, parasympathetic and sympathetic ganglia, with the strongest expression in the superior cervical ganglion, and low to moderate levels were detected in brain and spinal cord, respectively, which rapidly increased in intensity with embryonic age. In addition, robust α7 mRNA expression was detected in the adrenal medulla, and low to moderate expression in selected peripheral tissues during embryonic development, potentially related to cells derived from the neural crest. Little or no mRNA expression was detected in thymus or spleen, sites of immune cell maturation. The results suggest that prenatal nicotine exposure could potentially affect the nervous system with limited effects in non-neural tissues.https://www.frontiersin.org/article/10.3389/fnana.2019.00076/fullcortexhippocampusspinal cordenteric nervous systemadrenal medullakidney |
| spellingShingle | Ron S. Broide Ursula H. Winzer-Serhan Yling Chen Frances M. Leslie Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse Frontiers in Neuroanatomy cortex hippocampus spinal cord enteric nervous system adrenal medulla kidney |
| title | Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse |
| title_full | Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse |
| title_fullStr | Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse |
| title_full_unstemmed | Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse |
| title_short | Distribution of α7 Nicotinic Acetylcholine Receptor Subunit mRNA in the Developing Mouse |
| title_sort | distribution of α7 nicotinic acetylcholine receptor subunit mrna in the developing mouse |
| topic | cortex hippocampus spinal cord enteric nervous system adrenal medulla kidney |
| url | https://www.frontiersin.org/article/10.3389/fnana.2019.00076/full |
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